Short-term stability of wastewater samples for storage and shipment in the context of the EU Sewage Sentinel System for SARS-CoV-2

Tavazzi, Simona; Cacciatori, C.; Comero, S.; Fatta‐Kassinos, Despo; Karaolia, Popi; Iakovides, I.C.; Loutsiou, P.; Gutiérrez-Aguirre, Ion; Lengar, Živa; Bajde, I.; Tenson, Tanel; Kisand, Veljo; Laas, Peeter; Panksep, Kristel; Tammert, Helen; Mariani, G.; Skejo, H.; Bernd, Gawlik

doi:10.1016/j.jece.2023.109623

Cited by 8 publications

(1 citation statement)

References 22 publications

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“…Since 2020, these techniques have become increasingly important due to the need to control the spread of SARS-CoV-2 (Barcellos et al, 2023). As with any research related to SARS-CoV-2, there is an extensive literature on its surveillance in wastewater (Amman and Bergthaler, 2022;Dutta et al, 2022;Gonçalves et al, 2022;Greenbaum et al, 2022;Kumar et al, 2022;Burdorf and Rugulies, 2023;Du Toit, 2023;Gahlot et al, 2023;Hopkins et al, 2023;Oloye et al, 2023;Sodhi and Singh, 2023;Tavazzi et al, 2023). This has resulted in a renaissance of WBE and early warning systems based on pathogen surveillance (O'Brien and Xagoraraki, 2019;Guo et al, 2022a;Guo et al, 2022b;Fitzmorris-Brisolara et al, 2022;Demeter et al, 2023;Hassard et al, 2023;Shaw et al, 2023;Wolfe et al, 2023) On the other hand, WBE also place significant emphasis on detecting small molecules and/or metabolites excreted by humans.…”

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confidence: 99%

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Investigation and detection of multiple antibiotic-resistant pathogenic bacteria in municipal wastewater of Dhaka city

Siddique,

Munni,

Hasan

et al. 2024

Discov Water

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Background Water pollution in densely populated urban areas, mainly from municipal wastewater, poses a significant threat. Pathogenic bacteria, such as Vibrio spp. and fecal coliform, endanger public health and the environment. Additionally, antibiotic-resistant bacteria in wastewater complicate treatment and heighten public health concerns. Methods The study sampled municipal wastewater from ten Dhaka neighborhoods, selecting treatment plants, sewage outlets, and various collection points using meticulous techniques for representative samples. Bacteriological and biochemical analyses were conducted using standardized methods. Antimicrobial susceptibility testing (AST) was performed with the disk diffusion method against 13 widely used antibiotics. Results All sampled areas exhibited positive results for Vibrio spp., fecal coliform, E. coli, and Salmonella spp. Varying bacterial concentrations were observed, with the highest concentration of TVC, total vibrio spp., and total fecal coliform, total E. coli count, and total Salmonella spp. were found in Uttara (1.9 × 104 CFU/ml), Bangshal (1.8 × 102 CFU/ml), and Lalbag (2.1 × 103 CFU/ml), Mirpur (3.70 × 102 CFU/ml), and Lalbag (6 × 102 CFU/ml) respectively. AST results revealed significant resistance among all bacterial species to various antibiotics. Specifically, Vibrio spp. showed 100% resistance to cefuroxime, fecal coliform exhibited 90% resistance to cephradine, E. coli demonstrated 60% resistance to cephradine, and Salmonella spp. displayed 90% resistance to ampicillin. Conclusion The study highlights the existence of multiple antibiotic-resistant bacteria in Dhaka's wastewater. Addressing antibiotic resistance is essential to manage the risks of multiple antibiotic-resistant infections and maintain antibiotic effectiveness. These implications are critical for various stakeholders, including public health officials, policymakers, environmentalists, and urban planners.

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