Short-term storage and cryopreservation of lake minnow (<i>Eupallasella percnurus</i>
 (Pallas, 1814)) sperm

Dietrich, Mariola A.; Wolnicki, Jacek; Słowińska, Mariola; Sikorska, Justyna; Hliwa, Piotr; Kamiński, Rafał; Liszewska, Ewa; Ciereszko, Andrzej

doi:10.1111/jai.12727

Cited by 9 publications

(3 citation statements)

References 15 publications

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“…Considering all the trials, seems to be evident that to dilute the sperm is an essential premise for keep the sperm motility as long as possible, always using a proper extender similar to the seminal plasma composition of target species. In other phylogenetically close species such as R. percnurus, Short-term storage of semen was successfully applied to secure 50% sperm motility for 9 days in VRT buffer (Dietrich et al, 2015).…”

Section: Short-and Long-term Gamete Storagementioning

confidence: 99%

“…As far as we know, there are only few reports about the gamete cryopreservation of leuciscid species. One of them was carried out in the lake minnow (E. percnurus), and the highest motility value of cryopreserved sperm was obtained applying methanol and dimethyl sulfoxide (DMSO) as cryoprotectants in Tris-glucose buffer (Dietrich et al, 2015). Other study carried out by Bernáth et al (2018) on the ide (L. leuciscus), showed no signi cant differences between fresh and cryopreserved sperm using 10% methanol, but the variability between sample were too high (pMOT; fresh sperm: 49 ± 19% versus and cryopreserved sperm: 22 ± 22%).…”

Section: Short-and Long-term Gamete Storagementioning

confidence: 99%

See 1 more Smart Citation

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

Hernández-Rodríguez

Sousa‐Santos

Gil

et al. 2023

Preprint

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During the spring of 2022 several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma. occidentale and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the first time in four species, using a computerised analysis system. The results obtained showed that spermatozoa kinetic patterns were similar for all 4 species, with high motility and velocity values after the sperm activation time and with a marked decrease after 20 seconds. On the other hand, sperm longevity was highly variable between species, with short longevities (around 40 s) for A. hispanica and S. aradensis, and longer longevities (100–120 s) for A. occidentale and I. lusitanicum, which could indicate a latitudinal pattern in terms of sperm longevity. At the same time, morphometric analysis was carried out for the four target species, revealing that spermatozoa showed similar sizes and shapes to other external fertilizers belonging to Leuscididae, with small spherical heads, uniflagellate and without acrosomes. In addition, different short-term gamete storage trails were performed by diluting sperm in 1:9 (sperm:extender) and storing them at 4ºC. Although the results obtained were uneven among the species studied, the dilution and extender used generated motilities above 40% up to day 4 of storage in S. aradensis and I. lusitanicum, and up to day 1–2 in A. hispanica and A. occidentale, respectively. Finally, gamete cryopreservation trials were also carried on these threatened species. Although cryopreserved samples showed significantly lower motility than fresh samples, some protocols generate acceptable percentages of viability, DNA integrity, and sperm motility in some species such as I. lusitanicum and A. occidentale. The data revealed that the protocol based on 10% DMSO plus 7.5% egg yolk generated the best results. This study is the first to assess the reproductive traits of wild and captive populations of endangered leuciscids endemic from the Iberian Peninsula, describing the spermatozoa kinetics and developing protocols for managing male gametes both in short- and long- term storage. Outcomes will provide new and useful tools to complement the management and conservation of ex-situ breeding programs that are being developed on these four endangered species.

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Section: Short-and Long-term Gamete Storagementioning

confidence: 99%

Section: Short-and Long-term Gamete Storagementioning

confidence: 99%

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

Hernández-Rodríguez

Sousa‐Santos

Gil

et al. 2023

Preprint

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show abstract

“…Maintaining a genetic diversity in populations of endangered species is an important part of conservation biology and its programs focused on maintaining biodiversity. It can be performed using techniques of gene banking, which are used in conservation of populations endangered because of anthropogenic pressure or the deterioration of environmental conditions [ 1 , 2 , 3 ]. The gene bank is a fragment of allelic diversity specific to a given population that has been conserved by using various techniques.…”

Section: Introductionmentioning

confidence: 99%

Management of Genetic Variation in the Gamete Bank of the Endangered Lake Minnow Eupallasella percnurus, Using Genassemblage 2.2 Software

Kaczmarczyk¹,

Wolnicki²

2022

Animals

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The management of genetic resources deposited in gene banks requires knowledge of the genetic profiles of the gamete donors and bioinformatics tools to process this information. In this work, we show how to use Genassemblage 2.2 software in managing the genetic variation deposited in a bank of cryopreserved semen. Our demonstration was based on the leuciscid fish species, lake minnow Eupallasella percnurus, which is designated as endangered in Poland. The semen samples (n = 192) were taken from four Polish lake minnow populations and frozen in the gene bank. Fin clips were taken and DNA extracted. Across 13 investigated microsatellite loci, 21–53 alleles were identified in each population and 66 in the entire group of samples. The module “Management of genetic variation in gamete bank” of Genassemblage 2.2 software was used to find the set of samples that will preserve 100% of the detected allelic diversity in the next generation. Our results showed that a small group of 4–19 semen samples was enough to transfer all alleles detected across this set of samples. We, therefore, recommend Genassemblage 2.2 as a convenient tool for the detection of genetic differences between donors, the construction of optimal sets of samples for conservation of genetic variation, and for managing genetic variation deposited in gamete banks. Consequently, it can be used in breeding human-dependent populations and to optimize the use of genetic diversity in samples in the gamete banks. It can be especially useful for conserving populations of species characterized by low genetic variation, such as the lake minnow.

show abstract

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long-term

Hernández-Rodríguez

Sousa‐Santos²,

Gil

et al. 2023

Fish Physiol Biochem

View full text Add to dashboard Cite

During the spring of 2022, several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma Occidentale, and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the first time in four species, using a computerized analysis system. The results obtained showed that spermatozoa kinetic patterns were similar for all 4 species, with high motility and velocity values after the sperm activation time and with a marked decrease after 20. On the other hand, sperm longevity was highly variable between species, with short longevities (around 40 s) for A. hispanica and S. aradensis, and longer longevities (100–120 s) for A. occidentale and I. lusitanicum, which could indicate a latitudinal pattern in terms of sperm longevity. At the same time, morphometric analysis was carried out for the four target species, revealing that spermatozoa showed similar sizes and shapes to other external fertilizers belonging to Leuscididae, with small spherical heads, uniflagellate, and without acrosomes. In addition, a short-term gamete storage trail was performed by diluting sperm in 1:9 (sperm:extender) and storing them at 4ºC. Although the results obtained were uneven among the species studied, the dilution and extender used generated motilities above 40% up to day 4 of storage in S. aradensis and I. lusitanicum, and up to days 1–2 in A. hispanica and A. occidentale, respectively. Finally, gamete cryopreservation trials were also carried out on these threatened species. Although cryopreserved samples showed significantly lower motility than fresh samples, some protocols generate acceptable percentages of viability, DNA integrity, and sperm motility in some species such as I. lusitanicum and A. occidentale. The data revealed that the protocol based on 10% DMSO plus 7.5% egg yolk generated the best results.This study is the first to assess the reproductive traits of wild and captive populations of endangered leuciscids endemic from the Iberian Peninsula, describing the spermatozoa kinetics and developing protocols for managing male gametes both in short- and long-term storage. Outcomes will provide new and useful tools to complement the management and conservation of ex situ breeding programs that are being developed for these four endangered species.

show abstract

Short-term storage and cryopreservation of lake minnow (Eupallasella percnurus (Pallas, 1814)) sperm

Cited by 9 publications

References 15 publications

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

Management of Genetic Variation in the Gamete Bank of the Endangered Lake Minnow Eupallasella percnurus, Using Genassemblage 2.2 Software

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long-term

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