Short-term storage of Sarotherodon mossambicus ova

Harvey, Brian; Kelley, R. N.

doi:10.1016/0044-8486(84)90304-1

Cited by 19 publications

(16 citation statements)

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“…Better storage are found in some cold-water species including salmonids (Barrett, 1951;Withler and Morley, 1968;Poon and Johnson, 1970;Jensen and Alderdice, 1984;Babiak and Dabrowski, 2003;Niksirat et al, 2007), sturgeons (Billard et al, 2004;Sohrabnezhad et al, 2006), flatfishes (Suquet et al, 1999) and walleye (Dietrich et al, 2012) although it is dependent on species and storage conditions (Rothbard et al, 1996;Linhart et al, 2001;Yasui et al, 2010). Based on our results, we conclude that oocytes from yellowtail tetra cannot be stored successfully, although other procedures may be used in future works including solutions with additives that may improve storage ability as was observed in the other warm-water fish such as the tilapia (Harvey and Kelley, 1984).…”

Section: Discussionmentioning

confidence: 77%

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Yasui

Senhorini

Shimoda

et al. 2015

Animal

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In fish, in vitro fertilization is an important reproductive tool used as first step for application of others biotechniques as chromosome and embryo manipulation. In this study, we aimed to optimize gamete quality and their short-term storage from the yellowtail tetra Astyanax altiparanae, for future application in laboratory studies. Working with sperm, we evaluated the effects of spawning inducers (carp pituitary gland and Ovopel ® [(D-Ala6, Pro9-NEt) -mGnRH + metoclopramide]) and the presence of female on sperm motility. Additionally, we developed new procedures for short-term storage of sperm and oocytes. Briefly, sperm motility was higher when male fish were treated with carp pituitary gland (73.1 ± 4.0%) or Ovopel ® (79.5 ± 5.5%) when compared with the control group treated with 0.9% NaCl (55.6 ± 27.2%; P = 0.1598). Maintenance of male fish with an ovulating female fish also improved sperm motility (74.4 ± 7.4%) when compared with untreated male fish (42.1 ± 26.1%; P = 0.0018). Storage of sperm was optimized in modified Ringer solution, in which the sperm was kept motile for 18 days at 2.5°C. The addition of antibiotics or oxygen decreased sperm motility, but partial change of supernatant and the combination of those conditions improve storage ability of sperm. Fertilization ability of oocytes decreased significantly after storage for 30, 60 90 and 120 min at 5, 10, 15 and 20°C when compared with fresh oocytes (P = 0.0471), but considering only the stored samples, the optimum temperature was 15°C. Those data describe new approaches to improve semen quality and gametes short-term storage in yellowtail tetra A. altiparanae and open new possibilities in vitro fertilization.Keywords: cryopreservation, sperm, oocyte, fish, yellowtail tetra ImplicationsThe yellowtail tetra Astyanax altiparanae is a characin fish largely distributed in Neotropical regions. As a small-sized intertidal spawner, this species is a promising candidate for laboratory and aquaculture technologies including polyploidy, chimerism and nuclear transplantation. However, the first step for such kind of studies is to establish a protocol for in vitro fertilization (IVF) at laboratory conditions. In this work several factors affecting gamete quality were evaluated, and the findings will collaborate for an effective protocol for IVF in this species.

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Section: Discussionmentioning

confidence: 77%

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Yasui

Senhorini

Shimoda

et al. 2015

Animal

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“…The time period during which eggs remain viable inside the fish body has been reported for a variety of species: 30 min for striped bass (Morone saxatilis) (Piper et al 1982), 1.5 hr for tilapia (Sarotherodon mossambicus) at 18-20 C (Harvey and Kelley 1984), 2 hr for Asian catfish (Pangasius hypophthalmus) at 28-29 C (Legendre et al 2000), 2 hr for the neotropical teleost fish, curimata, at both 18 and 26 C (Rizzo et al 2003), at least 3 hr for Senegalese sole (Solea senegalensis) at 16 C (Rasines et al, 2012), 9 hours at 20 C and 5 hr at 24 C for the South American catfish (Rhamdia sapo) (Espinach et al 1984), and 12 hr for goldfish (Formacion et al 1993). Complete loss of egg viability in kutum (Rutilus frisii kutum) was observed 72-96 hr postovulation at 11 C and 60-72 hr postovulation at 14 C (Samarin et al, 2011a).…”

Section: Egg Viability Rete Changes Through Oocyte Ageingmentioning

confidence: 98%

Fish Oocyte Ageing and its Effect on Egg Quality

Samarin

Policar

Lahnsteiner

2015

Reviews in Fisheries Science & Aquaculture

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In fish, delayed spawning in nature, delayed egg collection in capture and delayed fertilization after egg stripping can lead to oocyte ageing and over-ripening. Oocyte ageing is reported as one of the most important factors affecting the egg quality. During over-ripening, morphological, physiological, biochemical, histological, cellular, and molecular changes occur inside the eggs and ovarian fluid. These alterations can negatively affect the egg fertilization capacity and successive developmental stages. The time period during which eggs remain viable after ovulation or stripping has been reported from a few minutes to a few weeks depending on the fish species and the storage temperature. Although several biomarkers that characterize the over-ripening phenomenon have been defined, the reliability of these parameters in the field of aquaculture is controversial. For future researches, inhibition of oocyte ageing by antioxidants could be a valuable research topic. In the present article, the morphological, physiological, biochemical, histological, cellular, and molecular changes that occur during oocyte over-ripening under in vivo conditions and during in vitro storage are reviewed and possible reasons for the decrease in viability of eggs are presented. Species-specific changes in the egg viability and larval development are also considered.

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“…The time period during which eggs remain viable after ovulation, which guarantees egg fertility, has been reported for a variety of species: 30 min for striped bass ( Morone saxatilis ) (Piper et al., ), 1.5 h for tilapia ( Sarotherodon mossambicus ) at 18–20°C (Harvey and Kelley, ), 2 h for Asian catfish ( Pangasius hypophthalmus ) at 28–29°C (Legendre et al., ) and for neotropical teleost fish Prochilodus marggravii at 18 and 26°C (Rizzo et al., ), 9 h at 20°C and 5 h at 24°C for the South American catfish ( Rhamdia sapo ) (Espinach Ros et al., ), 12 h for gold fish ( Carassius auratus ) (Formacion et al., ), 12 h at 14°C for kutum ( Rutilus frisii kutum ) (Samarin et al., ), 5–15 days for rainbow trout ( Oncorhynchus mykiss ) at 8–17°C (Sakai et al., ; Springate et al., ; Lahnsteiner, ; Azuma et al., ; Aegerter and Jalabert, ; Samarin et al., ) and 30–40 days for Caspian brown trout ( Salmo trutta caspius ) at 7°C (Bahrekazemi et al., ). As shown in the aforementioned studies, the acceptable post‐ovulatory stripping time differs from species to species, ranging from a few hours for warm‐water spawning species to a few weeks for cold‐water species.…”

Section: Discussionmentioning

confidence: 98%

“…The time period during which eggs remain viable after ovulation differs from species to species and depends largely on temperature (e.g. Piper et al., ; Espinach Ros et al., ; Harvey and Kelley, ; Formacion et al., ; Legendre et al., ; Rizzo et al., ; Samarin et al., ). The maximum time period between ovulation and the deterioration of the egg quality for common carp has been reported to be 50–80 min (Woynarovich and Horváth, ; Rottmann et al., ).…”

Section: Introductionmentioning

confidence: 99%

Determination of the best post-ovulatory stripping time for the common carp (Cyprinus carpioLinnaeus, 1758)

et al. 2015

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To determine the best time interval between ovulation and controlled egg stripping in female common carp (Cyprinus carpio), ovulated eggs were retained at 20°C inside the fish body until 12-14 h post-ovulation (HPO). The eggs of four females were stripped with two-hour intervals after ovulation for 12-14 HPO and fertilized with mixed milt obtained from three males. Eyeing, hatching and eyed-egg mortality rates as well as the occurrence of larval malformations and ploidy anomalies were considered as indices to assess egg quality. The results indicated that throughout 4-6 HPO the eyeing and hatching rates remained approximately 90 and 80% of the initial rates, respectively. Although not significantly different (P > 0.05), the viability rates (eyeing and hatching percentages) increased to about 7.5% of the initial rates at 2-4 HPO compared with 0-2 HPO. Thereafter, the eyeing and hatching rates decreased over time linearly and finally dropped to 11 and 2.5% in eggs fertilized at 12-14 HPO, respectively. Eyed-egg mortality and larval malformation rates did not display any marked increase in eggs fertilized up to four hours after ovulation but thereafter increased significantly; more than 50% of eyed eggs died, and approx. 20% of hatched larvae were malformed at 8-10 HPO. Post-ovulatory oocyte ageing did not affect the ploidy level of the larvae. Based on the results obtained in this study, common carp egg quality is maintained inside the fish body for at least four HPO. The best post-ovulatory stripping time was estimated to be within 2-4 HPO and therefore we recommend that the eggs be retained inside the fish body for this period of time after ovulation. The complete loss of egg viability also occurs 12-14 h after ovulation.

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Short-term storage of Sarotherodon mossambicus ova

Cited by 19 publications

References 4 publications

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Fish Oocyte Ageing and its Effect on Egg Quality

Determination of the best post-ovulatory stripping time for the common carp (Cyprinus carpioLinnaeus, 1758)

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