Short-term storage of tripronucleated human embryos

Grau, N.; Aparicio, Belén; Escrich, L.; Mercader, Amparo; Delgado, A.; Remohı́, José; Escribá, Marı́a-José

doi:10.1007/s10815-013-0036-8

Cited by 3 publications

(1 citation statement)

References 48 publications

(72 reference statements)

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“…Cooling to refrigeration temperatures (around 4–5 °C) not only inhibits embryo metabolism but also maintains embryo viability in several species, including bovine 7–9 , ovine 10 , rabbit 11,12 , and rodent 13–15 species and humans 16 . However, such cooled storage shows that embryonic survival rates differ among species, embryo stages and laboratory procedures.…”

Section: Introductionmentioning

confidence: 99%

Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C

Martínez

Cambra

Nohalez

et al. 2019

Sci Rep

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Vitrification is the ideal method for long-lasting storage of porcine embryos. However, both strict airline regulations for transport of liquid nitrogen dewars and the technical problems experienced when vitrified embryos are transferred using non-surgical procedures have led to the introduction of alternative storage methods, such as preserving embryos in liquid state. This study evaluated whether a pH-stable medium containing high concentrations of either foetal calf serum (FCS; 50%) or BSA (4%) combined with storage at temperatures of 17 °C or 20 °C maintained in vivo -derived morulae and blastocysts alive and unhatched (a sanitary requirement for embryo transportation) during 72 h of storage. Neither FCS nor BSA supplements were able to counteract the negative effect of low temperatures (17 °C) on embryonic survival after storage. At 20 °C, the protective effect of FCS or BSA depended on embryo stage. While FCS successfully arrested embryo development of only blastocysts, BSA arrested the development of both morulae and blastocysts. Over 80% of BSA arrested embryos restarted development by conventional culture and progressed to further embryonic stages, including hatching. In conclusion, porcine morulae and blastocysts can survive and remain unhatched during at least 72 h when stored at 20 °C in a BSA-containing medium.

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Section: Introductionmentioning

confidence: 99%

Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C

Martínez

Cambra

Nohalez

et al. 2019

Sci Rep

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pH, Temperature, and Light

Pomeroy

Reed

2020

Textbook of Assisted Reproduction

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ROCK Inhibitor During Hypothermic Storage Improves Re-Expansion Rate and Quality of Goat Blastocysts

Saberi

Forouzanfar

Nasr-Esfahani

2018

Biopreservation and Biobanking

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Embryos can be produced in nonheat stress conditions and transported by air to designated destinations using hypothermic and chilled storage procedures. Therefore, improvement of efficiency of these processes may have an important impact on the dairy industry. The aim of this study was to evaluate the viability of embryos treated with ROCK inhibitor (Y-27632) during 4 days of hypothermic storage of goat blastocysts. In this study, vitrification was used as a standard model of cryopreservation. Treatment with ROCK inhibitor (Y-27632) significantly improved the re-expansion rate of blastocyst postwarming compared with treatment with Y-26732, but it was lower than the re-expansion rate in vitrified/warmed blastocysts. The quality of blastocysts in terms of total cell number (TCN) was significantly higher in the control group than in treatment groups, but it was similar between Y-26732, Y-27632, and vitrification groups. The relative expression of BAX, as a proapoptotic marker, was similar between all the groups, whereas the relative expression of BCL2 as an antiapoptotic marker was significantly higher in the Y-26732 group. The rate of TUNEL positive cells was similar between Y-26732 and Y-27632 groups. Thus, our results reveal that addition of Y-27632 improves the re-expansion rate of hypothermic stored embryos possibly through stabilizing the cytoskeleton structure such as intermediate filaments, which prevents the formation of cell membrane blebbing and cytoplasmic fragmentation.

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Short-term storage of tripronucleated human embryos

Abstract: TPN human cleavage embryos and blastocysts can be successfully stored short-term for up to 24 h at 4 °C without using cryoprotectants without any significant negative impact on survival or subsequent in vitro development.

Cited by 3 publications

References 48 publications

Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C

Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C

pH, Temperature, and Light

ROCK Inhibitor During Hypothermic Storage Improves Re-Expansion Rate and Quality of Goat Blastocysts

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