Short-term study of retinal pigment epithelium sheet transplants onto Bruch's membrane

Wang, Hao; Yagi, Fumihiko; Cheewatrakoolpong, Noounanong; Sugino, Ilene K.; Zarbin, Marco A.

doi:10.1016/j.exer.2003.09.024

Cited by 23 publications

(22 citation statements)

References 40 publications

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“…8. After 1 month, the areas of non-perfusion seen in the early phase of the angiogram at 15 min have disappeared gery and this leakage ceases with time [4,6,7,8,9,12]. Although we found reduced leakage with time after debridement, we also observed that slight leakage could be detected for months after surgery.…”

Section: Discussioncontrasting

confidence: 46%

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Changes in the choroidal circulation of rabbit following RPE removal

Ivert

Kong

Gouras

2003

Graefe's Arch Clin Exp Ophthalmol

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Removal of the RPE causes changes throughout the underlying choroid. It reduces the circulation in the large choroidal vessels as well as the choriocapillaris. If large areas of RPE are removed, this choroidal non-perfusion can be permanent due to fibroblastic infiltration. Small areas of RPE removal or slight pressure on the retina lead to only transient reduction of the choroidal flow. The rapidity with which the choroidal blood flow can be reduced implies a reflex mechanism that responds to sudden RPE pressure and/or trauma. These changes are best observed with ICG angiography.

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Section: Discussioncontrasting

confidence: 46%

“…There is a hypothesis postulating that degeneration of the RPE leads to secondary degeneration of the choriocapillaris [4,7,8,12]. This secondary degeneration is thought to be due to a loss of a trophic influence that normal RPE exerts on the choriocapillaris, presumably on the endothelial cells forming this structure.…”

Section: Introductionmentioning

confidence: 98%

Changes in the choroidal circulation of rabbit following RPE removal

Ivert

Kong

Gouras

2003

Graefe's Arch Clin Exp Ophthalmol

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“…In addition, RPE cells lose their pigment with cell division. 31,32 Studies by Wang et al 24 show that at 3 days, transplanted RPE microaggregates show some signs of proliferation, as evidenced by positive Ki-67 labeling and the presence of mitotic cells. Labeling with carbon particles 33,34 or with fluorochromes 8,30,35 can present the same problems.…”

mentioning

confidence: 97%

“…One of the earliest and easiest methods used to identify transplanted RPE cells was pigmentation (i.e., transplantation of pigmented RPE cells into recipients who had no pigmented RPE). 6,12,[23][24][25][26][27][28] Although this method may be suitable for short-term studies, release of pigment into the subretinal space 26 and subsequent phagocytosis of pigment by native RPE cells 29,30 prevents unambiguous identification of the transplanted cells. In addition, RPE cells lose their pigment with cell division.…”

mentioning

confidence: 99%

Adeno-Associated Virus Encoding Green Fluorescent Protein as a Label for Retinal Pigment Epithelium

Hansen¹,

Sugino²,

Yagi³

et al. 2003

Invest. Ophthalmol. Vis. Sci.

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“…BM is a unique tissue with a thickness of 2e4 mm that serves as a molecular sieve to partially regulate the reciprocal exchange of nutrients, fluids, oxygen, and metabolic waste between the outer retina and the choroidal blood supply. It also acts as support for intact and functional cell sheet formation [6]. RPE dysfunction or cell loss is one of the major pathological changes leading to a wide range of retinal degenerative diseases, such as age-related macular degeneration (AMD) and some forms of retinitis pigmentosa (RP), which are the leading cause of blindness worldwide.…”

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confidence: 99%

A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells

et al. 2014

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a b s t r a c tVarious artificial membranes have been used as scaffolds for retinal pigment epithelium cells (RPE) for monolayer reconstruction, however, long-term cell viability and functionality are still largely unknown. This study aimed to construct an ultrathin porous nanofibrous film to mimic Bruch's membrane, and in particular to investigate human RPE cell responses to the resultant substrates. An ultrathin porous nanofibrous membrane was fabricated by using regenerated wild Antheraea pernyi silk fibroin (RWSF), polycaprolactone (PCL) and gelatin (Gt) and displayed a thickness of 3e5 mm, with a high porosity and an average fiber diameter of 166 ± 85 nm. Human RPE cells seeded on the RWSF/PCL/Gt membranes showed a higher cell growth rate (p < 0.05), and a typical expression pattern of RPE signature genes, with reduced expression of inflammatory mediators. With long-term cultivation on the substrates, RPE cells exhibited characteristic polygonal morphology and development of apical microvilli. Immunocytochemisty demonstrated RPE-specific expression profiles in cells after 12-weeks of co-culture on RWSF/PCL/Gt membranes. Interestingly, the cells on the RWSF/PCL/Gt membranes functionally secreted polarized PEDF and phagocytosed labeled porcine POS. Furthermore, RWSF/PCL/Gt membranes transplanted subsclerally exhibited excellent biocompatibility without any evidence of inflammation or rejection. In conclusion, we established a novel RWSF-based substrate for growth of RPE cells with excellent cytocompatibility in vitro and biocompatibility in vivo for potential use as a prosthetic Bruch's membrane for RPE transplantation.

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Short-term study of retinal pigment epithelium sheet transplants onto Bruch's membrane

Cited by 23 publications

References 40 publications

Changes in the choroidal circulation of rabbit following RPE removal

Changes in the choroidal circulation of rabbit following RPE removal

Adeno-Associated Virus Encoding Green Fluorescent Protein as a Label for Retinal Pigment Epithelium

A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells

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