Short-term toxicity of dibutyl phthalate to mice intestinal tissue

Abstract

The objective of this study was to investigate changes in intestinal histopathology and expression of heat-shock proteins (HSPs) in the small intestinal tissue of mouse after acute exposure to dibutyl phthalate (DBP). Forty-eight 60-day-old Institute of Cancer Research (ICR) mice were administered DBP by gavage once a day for 10 days. The mice were divided into three groups of 16 mice each: the high-dose group was administered 500 mg/kg body weight (BW) DBP; the low-dose group was administered 50 mg/kg BW; and the control group was not administered DBP. Significant increases in the uterine index, ovary index, and testicular index were observed in the DBP-exposed groups compared to those in the control group. Villus height and V/ C ratio significantly increased ( p < 0.05) in the duodenum and decreased ( p < 0.05) in the jejunum after the administration of DBP. The goblet cell number decreased in both the duodenum and the jejunum of mice exposed to DBP ( p < 0.05) compared to the number in the control group mice. Damage to the structure of the small intestine was accompanied by a marked increase in HSP27 expression and a decrease in the expression of HSP70 and HSP90 in both high-dose and low-dose groups. These results indicate that elevated HSP27 levels in the duodenum and jejunum may be important markers for acute DBP exposure and that HSP27 may act as a protective protein involved in intestinal mucosa repair.

Subacute exposure to di-isononyl phthalate alters the morphology, endocrine function, and immune system in the colon of adult female mice

“…In the present study, we showed that DiNP exposure significantly increased histological damage in the colon compared to controls. These results are consistent with previous studies that showed that subacute DBP exposure at 500 mg/kg/day significantly altered intestinal histopathology 41 . Specifically, DBP exposure significantly increased villus height and villus height/crypt depth ratio (V/C ratio) in the duodenum, whereas DBP exposure significantly decreased villus height and V/C ratio in the jejunum compared to control 41 .…”

“…These results are consistent with previous studies that showed that subacute DBP exposure at 500 mg/kg/day significantly altered intestinal histopathology 41 . Specifically, DBP exposure significantly increased villus height and villus height/crypt depth ratio (V/C ratio) in the duodenum, whereas DBP exposure significantly decreased villus height and V/C ratio in the jejunum compared to control 41 . DBP exposure at 50 mg/kg/day did not alter histopathology in the small intestine 41 .…”

“…Specifically, DBP exposure significantly increased villus height and villus height/crypt depth ratio (V/C ratio) in the duodenum, whereas DBP exposure significantly decreased villus height and V/C ratio in the jejunum compared to control 41 . DBP exposure at 50 mg/kg/day did not alter histopathology in the small intestine 41 . In our study, we also observed significantly altered histopathological changes due to subacute DiNP exposure; however, these significant changes were observed in the colon at 0.02, 0.2, 2, and 200 mg/kg DiNP.…”

Potential effect of fish oil to preserve expression of cell cycle and tight junction regulating genes in colon after di-isononyl phthalate ingestion in albino Wistar rats

“…Current study showed a severe oedema and sloughing of the colon wall with inflammatory cells infiltration in DIP-group. This comes in consistent with Yu et al, 15 who reported the same changes in the small intestine. The antioxidant effect of FO was well documented, 16 due to its content of EPA and DHA, which may provide an explanation to the normal histological architecture noticed in DIP-FO-group.…”

Cytogenetic Effect of Di- Butyl Phthalate on Adult Male Albino Rats and The Possible Protective Role of Ginger and Selenium Against This Effect

“…Di-butyl phthalate is widely used in many consumer products such as papers, paints, printing inks, adhesives, glass fibers [5], food packaging materials food containers, and toys. It also used in cosmetics, nail varnish, furniture and dye solvents [6]. Also, it is used in medications and medical devices such as tubing, external feeding bags, peritoneal dialysis bags, infusion tubing, oxygen masks, blood bags and catheters [7].…”