Short‐term toxicity studies of Sanguinarine and of two alkaloid extracts of<i>Sanguinaria Canadensis</i>L.

Becci, Peter J.; Schwartz, Herman; Barnes, H H; Southard, G. L.

doi:10.1080/15287398709530972

Cited by 56 publications

(36 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oral median lethal dose is 329 mg/kg body weight in mice and 100 mg/kg body weight in dogs, doses well above that needed to treat HP infections (Lampe, 1992). As for the safety of sanguinarine, no adverse effects have been observed in animals treated with up to 100 mg/kg, and the oral median lethal dose in rats was 1658 mg/kg, thus again demonstrating low in vivo toxicity (Becci et al, 1987;Keller and Meyer, 1989).…”

Section: Discussionmentioning

confidence: 90%

In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis

Mahady

Pendland

Stoia

et al. 2003

Phytotherapy Research

114

View full text Add to dashboard Cite

Methanol extracts of the rhizomes of Sanguinaria canadensis, and the roots and rhizomes of Hydrastis canadensis, two plants used traditionally for the treatment of gastrointestinal ailments, were screened for in vitro antibacterial activity against 15 strains of Helicobacter pylori. The rhizome extracts, as well as a methanol extract of S. canadensis suspension-cell cultures inhibited the growth of H. pylori in vitro, with a MIC50 range of 12.5-50.0 microg/ml. Three isoquinoline alkaloids were identified in the active fraction. Sanguinarine and chelerythrine, two benzophenanthridine alkaloids, inhibited the growth of the bacterium, with an MIC50 of 50.0 and 100.0 microg/ml, respectively. Protopine, a protopine alkaloid, also inhibited the growth of the bacterium, with a MIC50 of 100 microg/ml. The crude methanol extract of H. canadensis rhizomes was very active, with an MIC50 of 12.5 microg/ml. Two isoquinoline alkaloids, berberine and beta-hydrastine, were identified as the active constituents, and having an MIC50 of 12.5 and 100.0 microg/ml, respectively.

show abstract

Section: Discussionmentioning

confidence: 90%

In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis

Mahady

Pendland

Stoia

et al. 2003

Phytotherapy Research

114

View full text Add to dashboard Cite

show abstract

“…toxicity for sanguinarine LD 50 = 29 mg/kg in rats and that for chelerythrine LD 50 = 18.5 mg/kg in mice (Walterova et al, 1995). In subchronic studies, minor evidence for treatment-related toxicity of QBA (doses > 30 mg/kg/day; rat, monkey) has been reported (Becci et al, 1987). No toxic effects were observed in rats fed up to 150 ppm SA in the diet for two weeks and in rats treated by gavage with up to 0.6 mg SA/kg body weight for 30 days.…”

mentioning

confidence: 99%

Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats

Psotová¹,

Večeřa²,

Zdařilová³

et al. 2006

Vet. Med.

View full text Add to dashboard Cite

ABSTRACT:The subchronic safety of sanguiritrin, a mixture of sanguinarine (SA) and chelerythrine (CHE) quaternary benzo[c]phenanthridine alkaloids (QBA), obtained from Macleaya cordata was assessed. Rats were fed a diet containing 120 ppm sanguiritrin (100 ppm QBA) for 109 days. The feed consumption and the animal weight were monitored. The content of QBA in selected tissues and plasma was determined using HPLC. It was evidenced that 2% of QBA were absorbed through the GIT while 98% were excreted in the feces. In plasma, bilirubin, urea, creatinine, glomerular filtration, AST, ALT, GMT, ALP and total antioxidant capacity were determined. In liver, GSH level, lipoperoxidation products, SOD and GPx activities and total amount of cytochrome P450 were evaluated. Damage to nuclear DNA was assessed; a 32 P-postlabeling assay proved that no DNA-adducts were detected in nuclear and mitochrondrial DNA in liver. No adverse effects were observed on rat organism. QBA had no influence on the gut mucosal epithelium, liver tissue and any biochemical parameters tested. Oxidative stress was not manifested during the experiment.

show abstract

“…No toxic effects were observed in rats fed up to 150ppm sanguinarine in the diet for 14d and in rats treated by gavage with up to 0.6mg/kg body weight for 30d (Becci et al, 1987). No toxic effects were found with a mixture of 60% sanguinarine and 30% chelerythrine at a dose of 2 and 100 ppm, respectively, (0.1 and 5 mg/kg LW) in the feed of pigs, which were observed for 90 days.…”

Section: Toxicologymentioning

confidence: 92%

“…All rats survived when sanguinarine was administered in the feed to the animals over 14 days in a dose of 15, 45 and 150 ppm, respectively (Becci et al, 1987). In some studies a liver toxicity was observed, when high doses (10mg/kg LW/day, i.p.)…”

Section: Safetymentioning

confidence: 99%

Study on the assessment of plants/herbs, plant/herb extracts and their naturally or synthetically produced components as ‘additives’ for use in animal production

Vienna

Graz

Hohenheim

et al. 2007

EFS3

View full text Add to dashboard Cite

A As ss se es ss sm me en nt t o of f p pl la an nt ts s/ /h he er rb bs s a an nd d e ex xt tr ra ac ct ts s I In nt tr ro od du uc ct ti io on n 5 5 M Mo on no og gr ra ap ph hs s o of f t th he e f fo ol ll lo ow wi in ng g s sp pe ec ci ie es s: : FOREWORDIn September 2003 the European Parliament and the Council of the European Union adopted the Regulation No. 1831/2003 on additives for use in animal nutrition. The purpose of this regulation is to establish a Community procedure for authorising the placing on the market and use of feed additives and to lay down rules for the supervision and labelling of feed additives and premixtures in order to provide the basis for the assurance of a high level of protection of human health, animal health and welfare, environment, and "users and consumers" interests in relation to feed additives, whilst ensuring the effective functioning of the internal market. Under the current regulation detailed guidelines for some family of products are completely missing, that it is the case of the plants/herbs, plant/herb extracts and their naturally or synthetically produced components. For this reason it is one of the priorities of the FEEDAP Panel to implement guidelines/guidance for these types of compounds.It has been observed that plant/herbs and their extracts are increasingly being used (and informally marketed) not only as sensory additives, but also for other purposes not covered by the legislation, notably better growth or feed conversion, improved meat quality, and for prophylactic purposes. There are strong indications that such use will further increase as replacement additives for the antibiotic growth promoters whose use will be prohibited by the end of 2005.Nowadays, a compiled list of these compounds or extracts does not exists although the European Federation of Animal Feed Additive Manufacturers (FEFANA) notes that some 280 plant extracts and a further 1700 chemically defined compounds have been used within Member States as sensory additives in animal feeds.The results of this study will be used by EFSA, in consultation with the European Commission, Member States and other stakeholders, to provide input for the development of guidance/guidelines documents for the authorisation of plants/herbs, plant/herb extracts and their naturally or synthetically produced components for use in animal nutrition. ASSESSMENT OF PLANTS/HERBS, PLANT/HERB EXTRACTS AND THEIR NATURALLY OR SYNTHETICALLY PRODUCED COMPONENTS AS "ADDITIVES" FOR USE INTRODUCTIONThe last two decades have seen a substantial increase in use of herbs/botanicals and their products not only as herbal medicinal products and for food supplements, but also in the field of animal nutrition. The use of herbal feed additives, which include essential oils and (exotic) herbal mixtures, is extensively promoted by the producers, but the scientific background underpinning their use often is limited. Consequently an adequate knowledge of quality control (content of active substance(s) stability), efficacy and safety is oft...

show abstract

Short‐term toxicity studies of Sanguinarine and of two alkaloid extracts ofSanguinaria CanadensisL.

Cited by 56 publications

References 14 publications

In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis

In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis

Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats

Study on the assessment of plants/herbs, plant/herb extracts and their naturally or synthetically produced components as ‘additives’ for use in animal production

Contact Info

Product

Resources

About