Short-Time Tuning of the Biological Activity of Functionalized Polyelectrolyte Multilayers

Benkirane‐Jessel, Nadia; Lavalle, Ph.; Hübsch, E.; Holl, Vincent; Senger, Bernard; Haïkel, Youssef; Voegel, Jean‐Claude; Ogier, Joëlle; Schaaf, Pierre

doi:10.1002/adfm.200400129

Cited by 78 publications

(71 citation statements)

References 40 publications

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“…direct contact with the reservoirs, but can internalize drugs through localized degradation of the PLGA layer. 133,134 A final application of PEMs to be discussed in tissue engineering involves living cells functionalized with multilayer patches. 50 This work represents an elegant combination of several PEM applications and techniques outlined in this review.…”

Section: Multifunctional Pems For Tissue Engineeringmentioning

confidence: 99%

Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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The layer-by-layer assembly of sequentially adsorbed, alternating polyelectrolytes has become increasingly important over the past two decades. The ease and versatility in assembling polyelectrolyte multilayers (PEMs) has resulted in numerous wide ranging applications of these materials. More recently, PEMs are being used in biological applications ranging from biomaterials, tissue engineering, regenerative medicine, and drug delivery. The ability to manipulate the chemical, physical, surface, and topographical properties of these multilayer architectures by simply changing the pH, ionic strength, thickness, and postassembly modifications render them highly suitable to probe the effects of external stimuli on cellular responsiveness. In the field of regenerative medicine, the ability to sequester growth factors and to tether peptides to PEMs has been exploited to direct the lineage of progenitor cells and to subsequently maintain a desired phenotype. Additional novel applications include the use of PEMs in the assembly of three-dimensional layered architectures and as coatings for individual cells to deliver tunable payloads of drugs or bioactive molecules. This review focuses on literature related to the modulation of chemical and physical properties of PEMs for tissue engineering applications and recent research efforts in maintaining and directing cellular phenotype in stem cell differentiation.

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Section: Multifunctional Pems For Tissue Engineeringmentioning

confidence: 99%

Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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“…[6][7][8][9] Our strategy for regenerating bone combines a synthetic electrospun nanofibrous membrane (ENM) composed of the US Food and Drug Administration (FDA)-approved poly(ε-caprolactone) (PCL) polymer, and the bioactive growth factor BMP-2 entrapped into polymer nanoreservoirs built atop the nanofibers according to layer-by-layer (LbL) technology and fortified. [10][11][12][13][14][15][16][17][18][19][20][21] In bone repair, for a small lesion, we do not need bone substitute, as, at this early stage, the regenerative medicine is suitable for regenerating bone tissue. In regenerative medicine, the European and American authorities have already approved the use of BMP-2 or BMP-7 for bone regeneration applications.…”

Section: Introductionmentioning

confidence: 99%

A living thick nanofibrous implant bifunctionalized with active growth factor and stem cells for bone regeneration

Eap¹,

Keller²,

Schiavi³

et al. 2015

IJN

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New-generation implants focus on robust, durable, and rapid tissue regeneration to shorten recovery times and decrease risks of postoperative complications for patients. Herein, we describe a new-generation thick nanofibrous implant functionalized with active containers of growth factors and stem cells for regenerative nanomedicine. A thick electrospun poly(ε-caprolactone) nanofibrous implant (from 700 μm to 1 cm thick) was functionalized with chitosan and bone morphogenetic protein BMP-7 as growth factor using layer-by-layer technology, producing fish scale-like chitosan/BMP-7 nanoreservoirs. This extracellular matrix-mimicking scaffold enabled in vitro colonization and bone regeneration by human primary osteoblasts, as shown by expression of osteocalcin, osteopontin, and bone sialoprotein (BSPII), 21 days after seeding. In vivo implantation in mouse calvaria defects showed significantly more newly mineralized extracellular matrix in the functionalized implant compared to a bare scaffold after 30 days’ implantation, as shown by histological scanning electron microscopy/energy dispersive X-ray microscopy study and calcein injection. We have as well bifunctionalized our BMP-7 therapeutic implant by adding human mesenchymal stem cells (hMSCs). The activity of this BMP-7-functionalized implant was again further enhanced by the addition of hMSCs to the implant (living materials), in vivo, as demonstrated by the analysis of new bone formation and calcification after 30 days’ implantation in mice with calvaria defects. Therefore, implants functionalized with BMP-7 nanocontainers associated with hMSCs can act as an accelerator of in vivo bone mineralization and regeneration.

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“…Such modularly functionalized coatings could, in the future, become potent tools for the modification of biomaterial surfaces as applied to implants, prostheses or in tissue engineering [4][5][6].…”

Section: Active Biomaterials For Local Drug or Gene Therapiesmentioning

confidence: 99%

Nanostructured Active Biomaterials for Tissue Engineering Applications

Ferrand¹,

Mendoza-Palomares²,

Fioretti³

et al. 2010

TOPROCJ

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Abstract:In recent years, considerable effort has been devoted to the design and controlled fabrication of nanostructured materials with functional properties. The layer by layer buildup of the polyelectrolyte multilayered films from oppositely charged polyelectrolytes [1] offers new opportunities for the preparation of functionalized biomaterial coatings. This technique allows the preparation of supramolecular nano-architectures exhibiting specific properties in terms of control of cell activation and may also play a role in the development of local drug or gene delivery systems. In our team, peptides, proteins or DNA, chemically bound to polyelectrolytes, adsorbed on or embedded in such architectures, have been shown to retain their biological activities. The challenge is now to develop a novel generation of multifunctional architectures and to use them for clinical applications.

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Short-Time Tuning of the Biological Activity of Functionalized Polyelectrolyte Multilayers

Cited by 78 publications

References 40 publications

Polyelectrolyte Multilayers in Tissue Engineering

Polyelectrolyte Multilayers in Tissue Engineering

A living thick nanofibrous implant bifunctionalized with active growth factor and stem cells for bone regeneration

Nanostructured Active Biomaterials for Tissue Engineering Applications

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