Short tryptophan- and arginine-rich peptide shows efficacy against clinical methicillin-resistant Staphylococcus aureus strains isolated from skin and soft tissue infections

Bacalum, Mihaela; Drăgulescu, Elena-Carmina; Necula, Gheorghe; Codiţă, Irina; Radu, Mihai

doi:10.1038/s41598-019-53926-4

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…We showed previously that the antimicrobial peptide P6 has a low toxicity against normal skin cells up to 200 µM [28], a concentration higher than the one we found to show effect against HCT-116 spheroids. Similar to our results, there were numerous tryptophan rich AMPs applied efficiently against cancer cells grown in monolayer [34][35][36][37].…”

Section: Pic Self-assemblies Increased Antitumor Potential Of P6 Against Hct-116 Spheroidsmentioning

confidence: 52%

“…P6 was synthetized by ThermoFisher Scientific/Pierce Biotechnology (Rockford, IL, USA) based on the structure reported previously [28]. Poly(ethylene oxide)-poly(acrylic acid), PEO5k-PAA3.2k (Mn=8200) was purchased from Polymer Source (Montreal, Canada) and used as received.…”

Section: Methodsmentioning

confidence: 99%

“…It forms a hydrophilic shell to protect the inner part of the self-assembly composed by the PAA block, negatively charged and thus able to bind with cationic AMP P6 (HRWWRWWRR-NH2). P6 is a peptide previously studied [6,28] which showed also good antimicrobial properties. This 9 amino acids long peptide has a net charge of +5, which gives it a higher affinity for bacterial and cancer cell membranes and 4 tryptophan residues which ensures a good anchoring into the lipid bilayer.…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Răileanu

Lonetti

Serpentini

et al. 2022

Journal of Molecular Structure

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Antimicrobial peptides, a large class of molecules synthetized by various organisms as an innate defense against pathogens are more and more used for their anticancer properties as well. In order to overcome some of their limitations and to enhance their therapeutic efficiency, the use of delivery systems was taken into consideration. In this study we describe an original delivery system for antimicrobial peptides based on its physico-chemical properties, namely the selfassembled polyion complexes (PIC) based on electrostatic interactions of cationic antimicrobial peptide P6 with negatively charged double hydrophilic block copolymers, the poly(ethylene oxide)-poly(acrylic acid) (PEO-PAA) in our study. The drug delivery system was tested on 3D human tumor HCT-116 spheroids. The spheroid evolution and cell viability were monitored at 24 and 48 h after the treatment was applied. Our study demonstrates for the first time the feasibility of forming a polyion complex PIC with an antimicrobial peptide and that this self-assembled organisation provides added value in terms of anti-tumour therapeutic efficacy compared to the free form of the antimicrobial peptide.

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Section: Pic Self-assemblies Increased Antitumor Potential Of P6 Against Hct-116 Spheroidsmentioning

confidence: 52%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Răileanu

Lonetti

Serpentini

et al. 2022

Journal of Molecular Structure

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Section: Peptide Defense Against S Aureusmentioning

confidence: 99%

“…These peptides demonstrated significant antibacterial activity and effectively inhibited biofilm formation, positioning them as a potential antibacterial activity for drug‐resistant S. aureus [174]. In addition, a newly synthesized peptide P6, rich in arginine and tryptophan, was effective against various MDR pathogens, including S. aureus strains [175]. Despite facing obstacles such as low therapeutic index, narrow spectrum of activity, and susceptibility to environmental factors like pH and temperature, AMPs exhibit one of the most advantageous properties.…”

Section: Innovative Therapeutic Strategies Against S Aureus and Resis...mentioning

confidence: 99%

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Gopikrishnan,

Haryini,

2024

J Basic Microbiol

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In recent years, antibiotic therapy has encountered significant challenges due to the rapid emergence of multidrug resistance among bacteria responsible for life‐threatening illnesses, creating uncertainty about the future management of infectious diseases. The escalation of antimicrobial resistance in the post‐COVID era compared to the pre‐COVID era has raised global concern. The prevalence of nosocomial‐related infections, especially outbreaks of drug‐resistant strains of Staphylococcus aureus, have been reported worldwide, with India being a notable hotspot for such occurrences. Various virulence factors and mutations characterize nosocomial infections involving S. aureus. The lack of proper alternative treatments leading to increased drug resistance emphasizes the need to investigate and examine recent research to combat future pandemics. In the current genomics era, the application of advanced technologies such as next‐generation sequencing (NGS), machine learning (ML), and quantum computing (QC) for genomic analysis and resistance prediction has significantly increased the pace of diagnosing drug‐resistant pathogens and insights into genetic intricacies. Despite prompt diagnosis, the elimination of drug‐resistant infections remains unattainable in the absence of effective alternative therapies. Researchers are exploring various alternative therapeutic approaches, including phage therapy, antimicrobial peptides, photodynamic therapy, vaccines, host‐directed therapies, and more. The proposed review mainly focuses on the resistance journey of S. aureus over the past decade, detailing its resistance mechanisms, prevalence in the subcontinent, innovations in rapid diagnosis of the drug‐resistant strains, including the applicants of NGS and ML application along with QC, it helps to design alternative novel therapeutics approaches against S. aureus infection.

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De Novo Design of Tryptophan Containing Broad‐Spectrum Cationic Antimicrobial Octapeptides

Sarkar,

Vignesh,

Kumar Sundaravadivelu

et al. 2024

ChemMedChem

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With the advent of antibiotic resistant organisms, development of alternate classes of molecules other than antibiotics to combat microbial infections, have become extremely important. In this context, antimicrobial peptides have taken center stage of antimicrobial therapeutic research. In this work, we have reported two cationic antimicrobial octapeptides WRL and LWRF, with broad spectrum antimicrobial activities against several strains of ESKAPE pathogens. Both the peptides were membrane associative and induced microbial cell death through membranolysis, being selective towards microbial membranes over mammalian membranes. The AMPs were unstructured in water, adopting partial helical conformation in the presence of microbial membrane mimics. Electrostatic interaction formed the primary basis of peptide‐membrane interactions. WRL was more potent, salt tolerant and faster acting of the two AMPs, owing to the presence of two tryptophan residues against that of one in LWRF. Increased tryptophan number in WRL enhanced its membrane association ability, resulting in higher antimicrobial potency but lower selectivity. This experimental and computational work, established that an optimum number of tryptophan residues and their position is critical for obtaining high antimicrobial potency and selectivity simultaneously in cationic AMPs. Understanding the peptide membrane interactions in atomistic details can lead to development of better antimicrobial therapeutics in future.

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Short tryptophan- and arginine-rich peptide shows efficacy against clinical methicillin-resistant Staphylococcus aureus strains isolated from skin and soft tissue infections

Cited by 7 publications

References 36 publications

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

De Novo Design of Tryptophan Containing Broad‐Spectrum Cationic Antimicrobial Octapeptides

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