Many exotic phenomena in strongly correlated electron systems emerge from the interplay between spin and motional degrees of freedom [1, 2]. For example, doping an antiferromagnet gives rise to interesting phases including pseudogap states and high-temperature superconductors [3]. A promising route towards achieving a complete understanding of these materials begins with analytic and computational analysis of simplified models. Quantum simulation has recently emerged as a complementary approach towards understanding these models [4][5][6][7][8]. Ultracold fermions in optical lattices offer the potential to answer open questions on the lowtemperature regime of the doped Hubbard model [9][10][11], which is thought to capture essential aspects of the cuprate superconductor phase diagram but is numerically intractable in that parameter regime. Already, Mott-insulating phases and short-range antiferromagnetic correlations have been observed, but temperatures were too high to create an antiferromagnet [12][13][14][15]. A new perspective is afforded by quantum gas microscopy [16][17][18][19][20][21][22][23][24][25][26][27][28], which allows readout of magnetic correlations at the site-resolved level [25][26][27][28]. Here we report the realization of an antiferromagnet in a repulsively interacting Fermi gas on a 2D square lattice of approximately 80 sites. Using site-resolved imaging, we detect (finite-size) antiferromagnetic long-range order (LRO) through the development of a peak in the spin structure factor and the divergence of the correlation length that reaches the size of the system. At our lowest temperature of T/t = 0.25(2) we find strong order across the entire sample, where the staggered magnetization approaches the ground-state value. Our experimental platform enables doping away from half filling, where pseudogap states and stripe ordering are expected, but theoretical methods become numerically intractable. In this regime we find that the antiferromagnetic LRO persists to hole dopings of about 15%, providing a guideline for computational methods. Our results demonstrate that quantum gas microscopy of ultracold fermions in optical lattices can now address open questions on the low-temperature Hubbard model.The Hubbard Hamiltonian is a fundamental model for spinful lattice electrons describing a competition between kinetic energy t and interaction energy U [29]. In the limiting case of half-filling (average one particle per site) and dominant interactions (U/t 1) the Hubbard model maps to the Heisenberg model [1]. There, the exchange energy J = 4t 2 /U can give rise to antiferromagnetically ordered states at low temperatures [30]. This order persists for all finite U/t, where charge fluctuations reduce the ordering strength [31]. Away from half-filling, the coupling between motional and spin degrees of freedom is expected to give rise to a rich many-body phase diagram (see Fig. 1a), which is challenging to understand theoretically due to the fermion sign problem [32]. Even so, in the thermodynamic limit com...