Shotgun Analysis of the Secretome of Fusarium graminearum

Abstract

Fusarium head blight, caused predominately by Fusarium graminearum, is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. To characterize the profile of proteins secreted by F. graminearum, the extracellular proteins were collectively obtained from F. graminearum culture supernatants and evaluated using one-dimensional SDS-PAGE and liquid chromatography-tandem mass spectrometry. A total of 87 proteins have been identified, of which 63 were predicted as secretory proteins including those with known functions. Meanwhile, 20 proteins that are not homologous to genomic sequences with known functions have also been detected. Some of the identified proteins are possible virulence factors and may play extracellular roles during F. graminearum infection. This study provides a valuable dataset of F. graminearum extracellular proteins, and a better understanding of the virulence mechanisms of the pathogen.

Large‐scale molecular genetic analysis in plant‐pathogenic fungi: a decade of genome‐wide functional analysis

“…Recently, Sperschneider et al (2016) have predicted 292 candidate effectors in this fungus, 42.5% of which are putative cytoplasmic effectors as their expression occurs in planta in wheat and barley. Other studies have identified 63 secreted proteins, many of which are hydrolytic enzymes, in F. graminearum (Ji et al, 2013). Apoplastic effectors belong to this group of enzymes as they can mediate plant cell breakdown at the apoplastic space.…”

Quantitative proteomic study of Aspergillus Fumigatus secretome revealed deamidation of secretory enzymes

“…has potential to produce RSDE. To explore the cellulose and lignocellulosic biomass hydrolysis potential and to investigate the abundance of lignocellulolytic enzymes, the proteomic analysis of secretome by biomass degrading microbes such as Thermobifida fusca [8], Trichoderma reesei [9][10][11], Phanerochaete chrysosporium [12,13], Botrytis cinerea [14,15], Postia placenta [16], Aspergillus niger [17] Fusarium graminearum [18] and many more have been documented. However, the post translational modifications (PTMs) of these proteins that could result in a huge impact on their functions have not been studied so far.…”

Identification and characterization of a NADPH oxidase target in Fusarium graminearum

“…A third, more recent study used shot-gun proteomics in an effort to detect new putative virulence factors. They grew Fusarium on minimal media lacking organic nitrogen and also found that FGSG_10089 is secreted (Ji et al, 2013). This is consistent with the presence of a secreted signal identified by bioinformatics analysis.…”

“…Several large scale genomic and proteomics studies in several environmental conditions in F. graminearum have identified FGSG_10089 as being expressed and secreted (Phalip et al, 2005;Yang et al, 2012;Ji et al, 2013). Proteomics analysis of secreted proteins of F. graminearum grown in medium containing cell wall derived from hops plants identified FGSG_10089 (Phalip et al, 2005).…”