Shotgun Metagenomic Survey of the Diseased and Healthy Maize (Zea mays L.) Rhizobiomes

Babalola, Olubukola Oluranti; Dlamini, Siphiwe Prudence; Akanmu, Akinlolu Olalekan

doi:10.1128/mra.00498-22

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Rhizosphere soil samples were collected from a cultivated lettuce ( Lactuta sativa L.) field in Gauteng Province, South Africa (26°05’14.9"S 27°37’08.6"E, altitude, 159 km). As earlier described [ 2 , 3 ], soil tightly-bound to plant root were scraped from each of the four lettuce varieties; Green Oak, Red Frilly, Salanova Green and Welton, while the bulk soil (control) was collected from a natural grassland 20 m away from the lettuce plantation. Using a calibrated scale, 500 mg of each collected soil samples was weighed while extraction of the whole community DNA was carried out using the NucleoSpin Soil kit (Macherey-Nagel, Germany) according to the manufacturers’ guidelines.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Dataset of shotgun metagenomic evaluation of lettuce (Lactuta sativa L.) rhizosphere microbiome

2023

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Dataset of shotgun metagenomic evaluation of lettuce (Lactuta sativa L.) rhizosphere microbiome

2023

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“…Their deployment for plant growth and disease management has been widely encouraged by soil scientists [23,68,69]. Thus, the natural soil microflora contain a variety of PGPR or PGPF [70], and they constitute a key element of integrated nutrient management with their application as biofertilizers that could be employed in sustainable agriculture. They can be suitably formulated for application through the seed or soil.…”

Section: Bioresources Uses As Biofertilizers and Biopesticidesmentioning

confidence: 99%

Bioresources in Organic Farming: Implications for Sustainable Agricultural Systems

Akanmu,

Olowe,

Phiri

et al. 2023

Horticulturae

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Over the years, the practice of agriculture has transformed from the era of traditional to that of intensive agriculture in the bid to boost the production index that will satisfy the food needs of the globally growing population. However, the continuous and exaggerated use of chemical fertilizers and pesticides has resulted in major adverse impacts on food and environmental safety, whereas most traditional techniques for reclamation of natural soil nutrients, including shifting cultivation and polyculture, are no longer attractive measures of land rejuvenation. There is, therefore, the need for urgent evaluation and adoption of innovative methods of replenishing the agricultural soils that conform to the current agricultural systems without exerting undesirable effects on the ecosystem. In this review, we elucidated the use of key bioresources, such as organic fertilizers, biofertilizers, and biopesticides, as alternatives to chemical-based products in attaining a safe and sustainable agricultural system. Bioresources are naturally available, safe, and easily accessible products. The potential of these biological products in fostering soil microbial growth, plants’ productivity, and induced host immunity to diseases, alongside the promotion of healthy soil–microbe–plant relationships and preservation of the ecosystem processes without disruption, are aspects that were also explored. Therefore, the productive use of bioresources is considered strategic as it pertains to attaining safe and sustainable food production.

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“…Despite its resilience, the growth and yield of maize are adversely affected by pests and diseases caused by fungi, viruses, nematodes and bacteria (Akanmu et al, 2023b;Dlamini et al, 2023b;Gadag et al, 2021). The infection of pathogenic organisms has been found to often result in significant yield losses (Akanmu et al, 2020;Dlamini et al, 2023b;Imade & Babalola, 2021), and fungi constitute an important domain of microorganisms infecting the cultivated maize (Babalola et al, 2022). Fungi causing the foliar diseases of maize have been reported across the world including grey leaf spot caused by Cercospora spp., common rust (Puccinia sorghi), leaf spot disease (Phoma herbarum), brown spot (Physoderma maydis), Phaeosphaeria leaf spot (Phaeosphaeria maydis), downy mildew (Peronosclerospora sorghi), Bipolaris zeicola (Helminthosporium leaf spot) and a range of foliar and root diseases caused by Fusarium verticillioides (Babalola et al, 2022;Belisário et al, 2022;Korsman et al, 2012;Liu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Characterization and biocontrol potential of some rhizobacteria against fungal pathogens causing foliar diseases in maize

Akanmu,

Babalola

2024

Studia Universitatis Babeş-Bolyai Biologia

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Maize is one of the most consumed cereal crops worldwide, and it is a strategic crop to the attainment of SDG 2 of Zero hunger. Despite its importance, the cultivation of maize has been significantly impaired by fungal pathogens causing foliar diseases. The occurrence of this disease in maize plantations at the Research Farm of the North-West University, Molelwane, Mafikeng, South Africa prompted this investigation. Samples of diseased maize rhizosphere soil were aseptically collected. Bacteria species associated with the rhizosphere were isolated and characterized as Bacillus siamensis, Enterobacter asburiae, Enterobacter chengduensis, Priestia aryabhattai, Burkholderia sp., Priestia megaterium strain AOA6 and Priestia megaterium strain AOA7. The anti-fungicidal potentials of the bacterial species were evaluated against pathogenic fungal species, Nigrospora sphaerica, Alternaria alternata and Fusarium equiseti in-vitro. The percentage mycelia growths were calculated and the data were subjected to ANOVA using SAS version 9.8. All the seven bacteria isolates tested positive to ammonia production, phosphate solubilization, siderophore production and ACC deaminase tests. The percentage mycelia inhibition showed Nigrospora sphaerica (36.29%), A. alternata (26.19%) and F. equiseti (20.63%) as the order of fungal inhibition by the bacteria species. Furthermore, E. asburiae > P. megatarium strain AOA7 > B. siamensis > P. aryabhattai > E. chengduensis > Bulkholderia sp. were the order of antifungal efficacy of the bacteria species evaluated. In conclusion, the efficacy of the bacteria especially E. asburiae, P. megatarium strain AOA7 and B. siamensis over various fungal pathogens. The result obtained, therefore, justifies the further investigation, formulation and deployment of the bacteria species as biofungicide in the management of foliar diseases of maize. Keywords: antifungicidal potential, biofungicide, microbial formulations, rhizosphere, zero hunger.

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Shotgun Metagenomic Survey of the Diseased and Healthy Maize (Zea mays L.) Rhizobiomes

Cited by 5 publications

References 4 publications

Dataset of shotgun metagenomic evaluation of lettuce (Lactuta sativa L.) rhizosphere microbiome

Dataset of shotgun metagenomic evaluation of lettuce (Lactuta sativa L.) rhizosphere microbiome

Bioresources in Organic Farming: Implications for Sustainable Agricultural Systems

Characterization and biocontrol potential of some rhizobacteria against fungal pathogens causing foliar diseases in maize

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