Shotgun proteomic analysis of nanoparticle-synthesizing Desulfovibrio alaskensis in response to platinum and palladium

Abstract

Proteomic studies on the response of D. alaskensis to platinum and palladium, the role these proteins have in the synthesis of nanoparticles, and subsequent increase in nanoparticle size after increased expression.

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

“…Copz is a chaperone protein with a role of intracellular sequestration and transport of Cu + from the cytoplasm to the periplasm ( 29 ). The p-type ATPase proteins are commonly described to be involved in bacterial resistance and toxicity mechanisms related to heavy metals ( 30 ). The remaining 11 proteins identified under growth conditions in the presence of copper sulfate were proteins that may be involved in biosynthesis and stabilization of the monoatomic copper.…”

Selective bacterial separation of critical metals a sustainable method for recycling lithium ion batteries

“…8a-b). 29 Characterisation of Co and Ni nanoparticles High resolution scanning transmission electron microscopy (STEM) was used to confirm that Co and Ni NPs were synthesised in the bacterial envelope. To our delight the characterisation revealed zero-valent Co NPs attached to the cells, the first time to our knowledge that such nanoparticles of this critical metal have been reported.…”

“…D. alaskensis G20 cultures for proteomic analysis were grown and washed in MOPS buffer similarly to the metal removal studies and previous proteomics analysis with this organism. 29 After 2 and 20 h incubation cell cultures were collected by centrifugation, the supernatants discarded and cell pellets stored at -75 °C until proteomic analysis. ESI-HPLC-MS/MS analysis Samples were reconstituted in 8 M urea and protein concentration was determined by Bradford protein assay (Biorad).…”

“…MS/MS spectra was searched using MASCOT Version 2.4 (Matrix Science Ltd, UK) against a custom D. alaskensis G20 database with maximum missed-cut value set to 2 as in previous proteomics work with this organism. 29 For convention the protein identifier (protein ID) used in the present work has been given the name of its encoding gene which starts with letters "Dde_" followed by four numeric digits. The updates related to genes and protein annotations can be found at the KEGG database (https://www.genome.jp/kegg/).…”

Micellar catalysis of the Suzuki Miyaura reaction using biogenic Pd nanoparticles fromDesulfovibrio alaskensis

“…occurs in the periplasm and generates biogenic nanoparticles that are transported to the cell surface and tightly associated to the outer membrane. 6 However, despite research into the mechanism 6 b and scalability of this process for remediation purposes, 7 the catalytic chemistry of metal nanoparticles generated by this microorganism is only now beginning to be explored. Desulfovibrio spp.…”