2011
DOI: 10.1016/j.jprot.2011.05.028
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Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri

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Cited by 56 publications
(51 citation statements)
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“…No phosphorylation was detected for the three key residues of S6K in our phosphoproteomic surveys. S6K was present, as phosphorylation at S61, S65, and S76 were detected and similar samples observed the unphosphorylated protein [26]. However, the lack of detected phosphorylation in O. tauri cannot be taken as contrary evidence as the quantity of observed phosphorylation in proportion to expected phosphorylation in O. tauri is still relatively low.…”
Section: Resultsmentioning
confidence: 75%
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“…No phosphorylation was detected for the three key residues of S6K in our phosphoproteomic surveys. S6K was present, as phosphorylation at S61, S65, and S76 were detected and similar samples observed the unphosphorylated protein [26]. However, the lack of detected phosphorylation in O. tauri cannot be taken as contrary evidence as the quantity of observed phosphorylation in proportion to expected phosphorylation in O. tauri is still relatively low.…”
Section: Resultsmentioning
confidence: 75%
“…It has already been used as a model for the eukaryotic cell-cycle, helping to unify current understanding of cell-cycle regulation across eukaryotes [23]. The lack of a cellulose plant cell wall facilitates transformation [24, 25] as well as organelle enrichment and protein extraction [26, 27]. These genetic and proteomic tools have already been applied to studies of protein turnover [27], nutrient deprivation [26] and the plant circadian clock in experimental [25, 28, 29] and mathematical approaches [30].…”
Section: Introductionmentioning
confidence: 99%
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“…Accordingly, M. pusilla CCMP1545 and O. tauri , two ancient green algae belonging to the Prasinophyceae [17, 18], have the smallest genomes, at 22 Mbp and 13 Mbp, respectively. In fact, of the available algal genome sequences, all members of the Prasinophyceae contain genomes that range in size from 12 to 22 Mbp [19].…”
Section: Resultsmentioning
confidence: 99%
“…The lack of a cellulose cell wall facilitates simple organelle enrichment and protein extraction, allowing large-scale analysis of the O. tauri proteome and phosphoproteome by massspectrometry. In O. tauri grown 12 h/12 h LD cycles, 27% of the predicted proteome was detected (97,98) , including phosphopeptides from proteins relevant to the circadian clock (CO, CRY1, LHY, TOC1, CK1 and GSK3). No forward genetic methods have been developed as yet, hampering the identification of clock 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 components that lack homology to those of other organisms, but a gene knock-out protocol using homologous recombination was recently published (95) .…”
Section: A Reduced Plant Circadian Clockmentioning
confidence: 99%