Background Patient safety and care quality are enhanced when healthcare professionals commit to expressing their concerns upon observing others’ mistakes, judgment lapses, or risky actions. Yet, available data show their speaking up behaviors may not be consistently practiced. Also less known is the most optimal approaches to teaching the important verbal and emotional skills for timely speaking up and respectful listening in interprofessional teams of healthcare professionals. Methods The authors developed a list of main tips for teaching speaking up and listening skills for promoting safety based on the following multiple sources of information: (a) literature reviews; (b) curriculum development in speaking up and listening; (c) delivery of training to a diverse group of interprofessional health professionals; and (d) participant evaluations of training effectiveness. Results We present twelve tips for teaching a holistic set of skills and mindsets encompassing both speaking up and listening, verbal strategies and emotional intelligence, and leaders’ and institutional commitment to promoting a psychologically safe clinical environment. Conclusions The twelve tips we outlined reflect a comprehensive approach to teaching skills in speaking up and listening within healthcare settings. By addressing the barriers to and facilitators of speaking up at the individual, team, and organizational levels, we recommend the practical strategies as important levers that promote healthcare professionals’ psychological safety and ultimately, a culture of safety in healthcare organizations.