Shp1 signalling is required to establish the long-lived bone marrow plasma cell pool

Li, Yanfeng; Xu, Shengli; Ou, Xijun; Lam, Kong-Peng

doi:10.1038/ncomms5273

Cited by 31 publications

(29 citation statements)

References 66 publications

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“…Two groups have reported a critical role for SHP-1 in GC maintenance and memory cell development [25, 26]. Although our studies here do not address SHP-1 recruitment in CD22 -/- GC B cells, they are consistent with the notion that the absence of CD22 leads to decreased SHP-1 recruitment.…”

Section: Resultssupporting

confidence: 86%

CD22 is required for formation of memory B cell precursors within germinal centers

2017

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CD22 is a BCR co-receptor that regulates B cell signaling, proliferation and survival and is required for T cell-independent Ab responses. To investigate the role of CD22 during T cell-dependent (TD) Ab responses and memory B cell formation, we analyzed Ag-specific B cell responses generated by wild-type (WT) or CD22-/- B cells following immunization with a TD Ag. CD22-/- B cells mounted normal early Ab responses yet failed to generate either memory B cells or long-lived plasma cells, whereas WT B cells formed both populations. Surprisingly, B cell expansion and germinal center (GC) differentiation were comparable between WT and CD22-/- B cells. CD22-/- B cells, however, were significantly less capable of generating a population of CXCR4hiCD38hi GC B cells, which we propose represent memory B cell precursors within GCs. These results demonstrate a novel role for CD22 during TD humoral responses evident during primary GC formation and underscore that CD22 functions not only during B cell maturation but also during responses to both TD and T cell-independent antigens.

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Section: Resultssupporting

confidence: 86%

CD22 is required for formation of memory B cell precursors within germinal centers

2017

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“…Membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Santa Cruz) and visualized with SuperSignal West Pico/Dura chemiluminescent substrate (Pierce)25.…”

Section: Methodsmentioning

confidence: 99%

Tyrosine kinase c-Abl regulates the survival of plasma cells

Huang

et al. 2017

Sci Rep

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Tyrosine kinase c-Abl plays an important role in early B cell development. Its deletion leads to reduced pro- and pre-B cell generation in mice. However, its function in B cell terminal differentiation remains unexplored. Here, we used c-Ablf/f Aicdacre/+ mice, in which c-Abl is ablated only in antigen-activated B cells, to study the role of c-Abl in germinal center (GC) B and antibody-secreting plasma cell formation. Upon challenge with a model antigen, we found normal GC and memory B but reduced plasma cells and antigen-specific antibody response in the mutant mice. In-vitro studies revealed that plasma cells lacking c-Abl could be generated but did not accumulate in culture, indicative of survival defect. They also exhibited impaired STAT3 phosphorylation. The plasma cell defects could be rectified by introduction of Bim-deficiency or delivery of colivelin, a STAT3 activator, into c-Ablf/f Aicdacre/+ mice. Hence, c-Abl signalling regulates the survival of plasma cells.

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“…4‐Hydroxy‐3‐nitrophenylacytyl (NP)‐specific IgM, IgG1 and IgG3 antibodies were determined by ELISA, and IgG1 antibody‐secreting cell (ASC) formation were detected by ELISPOT, following previously published protocols …”

Section: Methodsmentioning

confidence: 99%

“…and IgG3 antibodies were determined by ELISA, and IgG1 antibody-secreting cell (ASC) formation were detected by ELISPOT, following previously published protocols. 33 For ELISA, 384-well flat-bottomed NUNC plates were coated overnight with NP 14 -BSA (5 lg/ml), washed and blocked for 2 hr with 2% BSA in PBS (blocking buffer) at room temperature. Sera samples were serially diluted with blocking buffer and incubated for 2 hr at room tem-perature.…”

Section: -Hydroxy-3-nitrophenylacytyl (Np)-specific Igm Igg1mentioning

confidence: 99%

Loss of miR‐182 affects B‐cell extrafollicular antibody response

et al. 2016

Immunology

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SummaryMicroRNAs have been shown to play a role in B-cell differentiation and activation. Here, we found miR-182 to be highly induced in activated B cells. However, mice lacking miR-182 have normal B-cell and T-cell development. Interestingly, mutant mice exhibited a defective antibody response at early time-points in the immunization regimen when challenged with a T-cell-dependent antigen. Germinal centres were formed but the generation of extrafollicular plasma cells was defective in the spleens of immunized miR-182-deficient mice. Mutant mice were also not able to respond to a T-cell-independent type 2 antigen, which typically elicited an extrafollicular B-cell response. Taken together, the data indicated that miR-182 plays a critical role in driving extrafollicular B-cell antibody responses.

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Shp1 signalling is required to establish the long-lived bone marrow plasma cell pool

Cited by 31 publications

References 66 publications

CD22 is required for formation of memory B cell precursors within germinal centers

CD22 is required for formation of memory B cell precursors within germinal centers

Tyrosine kinase c-Abl regulates the survival of plasma cells

Loss of miR‐182 affects B‐cell extrafollicular antibody response

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