Shrinking Hydrogel‐DNA Spots Generates 3D Microdots Arrays

Goff, Gaelle C. Le; Blum, Loı̈c J.; Marquette, Christophe A.

doi:10.1002/mabi.201200370

Cited by 21 publications

(20 citation statements)

References 26 publications

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“…The GLAD-MACE platform is highly hydrophilic so while it can fully reap the benefits of increased surface area (due to 3D and porous surface) for attachment of analytes, extending the detection limit to fmol region without amplification, wicking is a severe problem for the miniaturization of the GLAD-MACE platform. The same is true generally for any hydrophilic substrate with 3D micro- or nanostructures as researchers continue to find innovative ways to combat the problem [ 13 ]. In this study, we aim to show the size detection sites can be shrink on superhydrophilic wicking surfaces with the example of GLAD-MACE substrate.…”

Section: Introductionmentioning

confidence: 99%

Photo-Attachment of Biomolecules for Miniaturization on Wicking Si-Nanowire Platform

Zheng

et al. 2015

PLoS ONE

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We demonstrated the surface functionalization of a highly three-dimensional, superhydrophilic wicking substrate using light to immobilize functional biomolecules for sensor or microarray applications. We showed here that the three-dimensional substrate was compatible with photo-attachment and the performance of functionalization was greatly improved due to both increased surface capacity and reduced substrate reflectivity. In addition, photo-attachment circumvents the problems induced by wicking effect that was typically encountered on superhydrophilic three-dimensional substrates, thus reducing the difficulty of producing miniaturized sites on such substrate. We have investigated various aspects of photo-attachment process on the nanowire substrate, including the role of different buffers, the effect of wavelength as well as how changing probe structure may affect the functionalization process. We demonstrated that substrate fabrication and functionalization can be achieved with processes compatible with microelectronics processes, hence reducing the cost of array fabrication. Such functionalization method coupled with the high capacity surface makes the substrate an ideal candidate for sensor or microarray for sensitive detection of target analytes.

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Section: Introductionmentioning

confidence: 99%

Photo-Attachment of Biomolecules for Miniaturization on Wicking Si-Nanowire Platform

Zheng

et al. 2015

PLoS ONE

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“…The high storage density, high parallelism and the natural advantage of the ultra-low energy consumption of the DNA molecule [24,17] make its potential in the field of storage and computing fully exploited. DNA molecular computing has an unique data storage and computing mechanism that could solve traditionally difficult problems [10,21] with a new perspective, while also providing new opportunities for information security [12,20]. DNA microarray (DNA chip) technology belongs to the category of biological chip technology.…”

Section: Introductionmentioning

confidence: 99%

A Visual Cryptography Scheme-Based DNA Microarrays

Zhang¹

2018

IJPE

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Visual cryptography is a cryptographic technique that allows visual information to be encrypted in such a way that the decryption can be performed by humans. The power of DNA molecule comes from its memory capacity and parallel processing. In this article, a visual encryption algorithm based on DNA microarrays is proposed, which successfully integrates the advantages of the algorithm in information security with the natural advantages of modern biotechnology. The algorithm converts plaintext into QR code and then uses the visual encryption scheme to encrypt the QR code image. It combines with DNA microarray technology to achieve information encryption and decryption. Security analysis shows that this algorithm has high security.

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“…A wide range of hydrogel chemical compositions have been explored for DNA or protein microarrays, in particular polyacrylamide [2, 16, 17], polyethylene glycol [18–20], and alginate [21] derivatives. Several methods to functionalize the gels have been explored, ranging from in situ functionalization at the time of synthesis to post-synthesis functionalization utilizing functional groups in the gel [22].…”

Section: Introductionmentioning

confidence: 99%

Hydrogel microparticles for biosensing

Goff

Srinivas

Hill³

et al. 2015

European Polymer Journal

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180

144

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Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field.

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Shrinking Hydrogel‐DNA Spots Generates 3D Microdots Arrays

Cited by 21 publications

References 26 publications

Photo-Attachment of Biomolecules for Miniaturization on Wicking Si-Nanowire Platform

Photo-Attachment of Biomolecules for Miniaturization on Wicking Si-Nanowire Platform

A Visual Cryptography Scheme-Based DNA Microarrays

Hydrogel microparticles for biosensing

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