shRNA Mediated RHOXF1 Silencing Influences Expression of BCL2 but not CASP8 in MCF-7 and MDA-MB-231 Cell Lines

Ghafouri‐Fard, Soudeh; Abdollahi, Davood Zare; Omrani, Mirdavood; Azizi, Faezeh

doi:10.7314/apjcp.2012.13.11.5865

Cited by 16 publications

(5 citation statements)

References 20 publications

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“…Ghafouri‐Fard et al . used pGFP‐V‐RS vector to silence GHOXF1 expression in two breast cancer cell lines; the efficiency of RNA inference was up to 80%. In addition, Zhou et al .…”

Section: Discussionmentioning

confidence: 99%

“…Zhang and his colleagues used pGenesil-1 vector to silence ezrin expression in MG63; however, the efficiency of RNA inference was only 60% 12 . Ghafouri-Fard et al used pGFP-V-RS vector to silence GHOXF1 expression in two breast cancer cell lines; the efficiency of RNA inference was up to 80% 13 . In addition, Zhou In the present study, we used pGFP-V-RS vector to silence ezrin expression.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Ezrin and Heat Shock Protein 70 on Apoptosis and Proliferation of Human Osteosarcoma Cells

Yao¹,

Xie

2015

Orthopaedic Surgery

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“…Ghafouri‐Fard et al . used pGFP‐V‐RS vector to silence GHOXF1 expression in two breast cancer cell lines; the efficiency of RNA inference was up to 80%. In addition, Zhou et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Ezrin and Heat Shock Protein 70 on Apoptosis and Proliferation of Human Osteosarcoma Cells

Yao¹,

Xie

2015

Orthopaedic Surgery

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“…RHOXF1 is a human gene of this family specifically expressed in reproductive tissues and different cancer cell lines. Also, the anti-apoptotic effects of its orthologue, as well as its hormone-dependent manner, increase its possible role not only in spermatogenesis but also in tu-morigenesis [10]. RT-PCR experiments have revealed RHOXF1 expression in the ovary, testis, epididymis, and prostate [5].…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of RHOXF1 Gene Expression in Different Cell Lines

2019

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Evaluating RHOXF1 Gene Expression in Different Cell LinesBackground: RHOXF1 transcription factor is highly expressed in reproductive tissues and probably plays a role in spermatogenesis. RHOXF1 is expressed in some cancer-derived cell lines, too. The antiapoptotic role of RHOXF1, together with its hormone-dependent function, may increase its probable role in tumorigenesis as well as spermatogenesis. RHOXF1 represents the properties of cancer-testis genes-the promising targets for immunotherapy. Therefore, its study in cancer cell lines can considerably contribute to future research on therapeutic interventions. This study used both experimental and in silico investigation of RHOXF1 gene expression in different cancer cell lines. Materials and Methods:In the present study, we investigated RHOXF1 in silico gene expression levels in breast cancer and prostate cancer cell lines as well as in the Human Protein Atlas, GEMiCCL, and Expression Atlas databases. We also evaluated HEK 293 cells using RT-PCR. Finally, the results were compared and analyzed. Results:The obtained data showed an increase in RHOXF1 in cilico expression levels in the MCF7 cell line, which has a clear contradiction with the Human Protein Atlas and GEMiCCL databases. In this study, RHOXF1 gene expression was not observed in HEK293, MDA-MB-231, PC3, and DU145. The expression of the RHOXF1 gene in the database was also absent in some other cell lines, including HEK 293. Conclusion:We discovered the discrepancy of some databases with the experimental findings, also we found higher expression levels of the RHOXF1 gene as a cancer-testis gene in the MCF7 breast cancer cell line, which can be investigated as a possible candidate for immunotherapy.

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“…CSCs are a group of cells within tumors which have stem-like features, express stem cell markers and are thought to be the sources of metastasis and tumor relapse [69]. Expression of CT genes in CSCs has clinical implications in the treatment of cancer recurrences and metastasis [70].…”

Section: Ny-eso-1 As a Stem Cell Markermentioning

confidence: 99%

New York Esophageal Squamous Cell Carcinoma-1 and Cancer Immunotherapy

Esfandiary

Ghafouri‐Fard

2015

Immunotherapy

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New York esophageal squamous cell carcinoma 1 (NY-ESO-1) is a known cancer testis gene with exceptional immunogenicity and prevalent expression in many cancer types. These characteristics have made it an appropriate vaccine candidate with the potential application against various malignancies. This article reviews recent knowledge about the NY-ESO-1 biology, function, immunogenicity and expression in cancers as well as and the results of clinical trials with this antigen.

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shRNA Mediated RHOXF1 Silencing Influences Expression of BCL2 but not CASP8 in MCF-7 and MDA-MB-231 Cell Lines

Cited by 16 publications

References 20 publications

Effects of Ezrin and Heat Shock Protein 70 on Apoptosis and Proliferation of Human Osteosarcoma Cells

Effects of Ezrin and Heat Shock Protein 70 on Apoptosis and Proliferation of Human Osteosarcoma Cells

The Evaluation of RHOXF1 Gene Expression in Different Cell Lines

New York Esophageal Squamous Cell Carcinoma-1 and Cancer Immunotherapy

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