shRNA-mediated silencing of ZFX attenuated the proliferation of breast cancer cells

Abstract

These findings suggest that ZFX plays an important role in breast cancer development and could be a potential therapeutic target for breast cancer.

Overexpression of ZFX and its involvement in squamous cell carcinoma of the tongue

“…We further revealed that ZFX expression was strongly associated with several clinicopathological factors in the tongue SCC specimens. Consistent with a previous study (20), we confirmed that ZFX expression was positively correlated with tumor grade and TNM classification of the tongue SCC, suggesting that ZFX overexpression was essential for the progression of tongue SCC. In addition, we revealed that ZFX overexpression was more frequent in male patients than that in female patients, suggesting a strong association between ZFX upregulation and gender.…”

“…Consistent with the results that ZFX knockdown caused cell cycle arrest in tongue SCC cell lines, previous studies showed that ZFX knockdown could inhibit the expression of cell cycle factors such as cyclin D1 and B1 in multiple types of cancer (18,20,21). Studies also found that ZFX depletion enhanced the expression of apoptotic factors including bax, caspase 1, 3 and 9 or inhibited the expression of anti-apoptotic factor such as bcl-2 in diverse cancers (12)(13)(14)18,20,21), which may account for the ZFX knockdown-induced cell apoptosis. Thus, it is quite possible that these mechanisms also function in tongue SCC, and further investigations are urgently needed to elucidate the mechanisms associated with ZFX-mediated tongue SCC development.…”

“…It has been shown that ZFX knockdown inhibits the phosphorylation of ERK1/2 and MEK1/2 in vitro and in vivo in gastric cancer. Furthermore, ERK and AKT activation have been observed in gliomas (18), NSCLC (21), LSCC (12) and breast cancers (20) following ZFX knockdown treatment. Consistent with the results that ZFX knockdown caused cell cycle arrest in tongue SCC cell lines, previous studies showed that ZFX knockdown could inhibit the expression of cell cycle factors such as cyclin D1 and B1 in multiple types of cancer (18,20,21).…”

“…Furthermore, ERK and AKT activation have been observed in gliomas (18), NSCLC (21), LSCC (12) and breast cancers (20) following ZFX knockdown treatment. Consistent with the results that ZFX knockdown caused cell cycle arrest in tongue SCC cell lines, previous studies showed that ZFX knockdown could inhibit the expression of cell cycle factors such as cyclin D1 and B1 in multiple types of cancer (18,20,21). Studies also found that ZFX depletion enhanced the expression of apoptotic factors including bax, caspase 1, 3 and 9 or inhibited the expression of anti-apoptotic factor such as bcl-2 in diverse cancers (12)(13)(14)18,20,21), which may account for the ZFX knockdown-induced cell apoptosis.…”

“…ZFX overexpression has been observed in variant types of cancer including human laryngeal SCC (LSCC) (12), prostate (13) and gastric cancer (14-16), human gliomas (17,18), non-small cell lung cancer (NSCLC) (19) and breast cancer (20). Consistent with these studies, here we showed that ZFX, a protein involved in survival and self-renewal of embryonic stem cells and implicated in the development of multiple types of cancer, was overexpressed in the tongue SCC samples.…”