Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells

Shu-Gan-Liang-Xue decoction simultaneously down-regulates expressions of aromatase and steroid sulfatase in estrogen receptor positive breast cancer cells

“…In peripheral tissues, estradiol is synthesized mainly through the aromatase or the steroid sulfatase pathway [9,11] . Our previous experiment confirmed that SGLXD dose-dependently inhibited the mRNA level and enzymatic activity of STS in MCF-7 cells [22] . However, there are some uncertain questions deserve further research.…”

“…The authenticated voucher specimens are available in our department. SGLXD was extracted by a routine method which is used in our laboratory as previous reports [21,22] . The SGLXD extract was diluted with culture mediums containing 10% fetal bovine serum (FBS, Gibco, Australia) to final concentrations (10,20,30,40, 50 mg/ml for MCF-7 and 5, 10, 15, 20, 25 mg/ml for T47D, respectively).…”

“…The human breast tissue has all important enzymes necessary to the synthesis, conversion and storage of 111111111111 activity [22] . In order to study more about the effects of SGLXD on the enzymes involved in the synthesis of estradiol, we performed the present experiment.…”

Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice

“…All were purchased from the Hospital Pharmacy of AHTCM. FJND was extracted by a routine method which is used in the Key Laboratory of Department of Integrated Traditional Chinese and Western Medicine, Peking University School of Oncology, as reported previously (11)(12)(13).…”

Current applications of molecular imaging and luminescence-based techniques in traditional Chinese medicine

“…The classic botanical formula was shown to response in transfected HeLa cells in a estrogen-specific manner, which induced increased expression of ER-Gal4-luciferase reporter gene in a dose-dependent manner (Miller-Martini et al, 2001). With a therapeutic mechanism similar to the Bupleurum and Peony Formula, the Shu-Gan-Liang-Xue Decoction (SGLXD) is composed of Bupleurum root, moutan and Lithospermum erythrorhizon and has been used to ameliorate hot flushes symptom (blood cooling) in breast cancer patients (Zhang and Li, 2010). To evaluate the estrogenic potential of SGLXD and the effect of SGLXD on cell viability of MCF-7 cells with dual-luciferase reporter assay and bioluminescent measurements, Zhang et al used transient cotransfection with estrogen-dependent plasmid palindromic estrogen response element (pERE)-TK-Luc and internal control plasmid pRL-TK in human mammary epithelial carcinoma cell line MCF-7 (Zhang and Li, 2009).…”