Sialic acid content and surface hydrophobicity of group B streptococci

Teixeira, Lenise Arneiro; Figueiredo, Agnes Marie Sá; Ferreira, Bernadete T.; Alves, V. M. L.; Nagao, Prescilla Emy; Alviano, Celuta Sales; Angluster, Jayme; Silva-Filho, F. C.; Benchetrit, Leslie C.

doi:10.1017/s0950268800050718

Cited by 9 publications

(7 citation statements)

References 23 publications

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“…El contenido de ácido siálico del polisacárido capsular es el principal factor de virulencia del serotipo III [24][25][26] . Cepas aisladas de infecciones invasivas en humanos muestran mayor contenido de ácido siálico capsular y son más resistentes a la fagocitosis que cepas aisladas como colonizantes.…”

Section: Discussionunclassified

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

et al. 2004

Rev. méd. Chile

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Section: Discussionunclassified

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

et al. 2004

Rev. méd. Chile

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“…CSH was determined by the affinity test to xylene (Teixeira et al , 1993). The hydrophobicity index (HI) was calculated using the following equation: where A 600 nm denotes the initial absorbance and B 600 nm represents the absorbance after vortex mixing.…”

Section: Methodsmentioning

confidence: 99%

Biofilm formation byAcinetobacter baumanniistrains isolated from urinary tract infection and urinary catheters

Pour

Dusane

Dhakephalkar

et al. 2011

FEMS Immunol Med Microbiol

133

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Fifty Acinetobacter isolates were obtained from urinary tract infections and urinary catheter samples. Analytical profile index assays identified 47 isolates as Acinetobacter baumannii and three as Acinetobacter lwoffii. Six A. baumannii isolates (A1-A6) displayed hydrophobicity indices >70%. Twenty isolates exhibited lectin activity. Biofilm formation by these isolates was compared with those with low hydrophobicity index values (A45-A50). Biofilms on different surfaces were confirmed by light microscopy, epifluorescence microscopy and by obtaining scanning electron microscope images. Biofilm production was maximal at 30 °C, pH 7.0 in a medium with 5.0 g L(-1) NaCl, and its efficiency was reduced on urinary catheter surfaces at sub-minimum inhibitory concentration concentrations of colistin. Plasmid-mediated antibiotic resistance was observed in selected isolates of A. baumannii and experiments of conjugation and transformation showed the occurrence of gene transfer. Plasmid curing was used to examine the function of plasmids. Five plasmids of A. baumannii A3 were cured but no differences were observed between wild-type and plasmid-cured strains with respect to the biofilm formation capabilities. The prevalence of A. baumannii strains with biofilm mode of growth could explain their ability to persist in clinical environments and their role in device-related infections.

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“…Bacterial cell surface hydrophobicity Hydrophobicity was determined by an affinity test to xylene [19], with bacterial cells from cultures in nutrient broth (Difco), previously incubated at 35-37ºC for 24 hours.…”

Section: Strainsmentioning

confidence: 99%

Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips

G¹,

Tognim²,

Cardoso³

et al. 2006

Braz J Infect Dis

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The cell surface hydrophobicity and adhesion to abiotic and cellular surfaces was tested in five clinical strains of Acinetobacter baumannii isolated from catheter tips. Biochemical and molecular characteristics of these strains were also studied. Hydrophobicity was characterized by a test for affinity to xylene. Adhesion to abiotic surfaces (polystyrene, formica, latex and glass) was evaluated in Petri plates using the stamp technique. Buccal epithelial cells were used for tests of adhesion to cellular surfaces. Adhesion to the catheter was evaluated by repeatedly rinsing the catheters and rolling them over nutrient agar. Molecular typing of the strains was done by the ERIC-PCR technique. The degree of hydrophobicity of the strains varied from hydrophobic to hydrophilic. All the strains adhered to the cell surfaces and to the catheters, and three of them strongly adhered to latex, polystyrene and formica. Catheter adhesion was reduced by meropenem. We found a direct relationship between the degree of bacterial hydrophobicity and adhesion to the abiotic surfaces, but not with adhesion to cellular surfaces, which suggests that different mechanisms are involved in adherence.

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Sialic acid content and surface hydrophobicity of group B streptococci

Cited by 9 publications

References 23 publications

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

Biofilm formation byAcinetobacter baumanniistrains isolated from urinary tract infection and urinary catheters

Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips

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