Sialic Acid Engineering of Thin Hydrogel Membranes

Abstract

We report the synthesis of glycosylated hydrogel membranes of poly{[N‐(2‐hydroxypropyl)methacrylamide]‐co‐(2‐hydroxyethyl methacrylate)} with the aim of developing bioactivated polymer substrates for cell culture. 3′‐Sialyllactose, the saccharidic portion of the GM3 ganglioside involved in cell‐cell recognition over a wide range of biological processes, was chemically modified with an acrylate group and incorporated into the growing macromolecular network of hydrogels by free radical crosslinking copolymerization. The incorporation and accessibility of the sialic acid residues at the hydrogel surface was verified by enzyme linked immunosorbent assay using mouse monoclonal anti‐GM3, and by electron microscopy after labeling with lectin‐gold nanoparticles. The water content was further characterized by thermogravimetry.magnified image

Influence of collagen addition on the thermal and morphological properties of chitosan/xanthan hydrogels

“…Since hydrogel biocompatibility can be attributed to their hydrophilicity and ability to retain a large amount of water [21], the water content of hydrogels (including the rehydrated hydrogels) was determined by thermogravimetry. The dehydration occurred as a single process, indicating that the weight loss correspond to interstitial water held in the pores.…”

Development of a Sialic Acid-Containing Hydrogel of Poly[N-(2-hydroxypropyl) methacrylamide]: Characterization and Implantation Study

“…The crystalline precipitate was isolated by filtration, washed three times with acetone, and dried in vacuo . Acrylated 3′-sialyllactose was prepared following the protocole described in Woerly et al…”

“…The crystalline precipitate was isolated by filtration, washed three times with acetone, and dried in vacuo. 13 Acrylated 3′-sialyllactose was prepared following the protocole described in Woerly et al 14 Synthesis of HPMA Hydrogels. The hydrogels were prepared by free radical polymerization of HPMA (33.68% w/v) in acetone/DMSO (93:7 v/v) with MbisAA as cross-linking agent (1.08 wt % of HPMA).…”