Sialic Acid in Semen, Spermatozoa and Serum of Mammals

Hudson, Marie T.; Wellerson, Ralph; Kupferberg, Alfred B.

doi:10.1530/jrf.0.0090189

Cited by 9 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that both blood and seminal plasma contain sialic acid and the levels are higher in semen compared to blood [ 66 ]. However, this is the first study to show that the sialic acid signature of body fluids is preserved in their EVs.…”

Section: Discussionmentioning

confidence: 99%

Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization

Kaddour

Panzner

Welch

et al. 2020

Viruses

View full text Add to dashboard Cite

Although extracellular vesicle (EV) surface electrostatic properties (measured as zeta potential, ζ-potential) have been reported by many investigators, the biophysical implications of charge and EV origin remains uncertain. Here, we compared the ζ-potential of human blood EVs (BEVs) and semen EVs (SEVs) from 26 donors that were HIV-infected (HIV+, n = 13) or HIV uninfected (HIV-, n = 13). We found that, compared to BEVs that bear neutral surface charge, SEVs were significantly more negatively charged, even when BEVs and SEVs were from the same individual. Comparison of BEVs and SEVs from HIV- and HIV+ groups revealed subtle HIV-induced alteration in the ζ-potential of EVs, with the effect being more significant in SEVs (∆ζ-potential = −8.82 mV, p-value = 0.0062) than BEVs (∆ζ-potential = −1.4 mV, p-value = 0.0462). These observations were validated by differences in the isoelectric point (IEP) of EVs, which was in the order of HIV + SEV ≤ HIV-SEV ≪ HIV + BEV ≤ HIV-BEV. Functionally, the rate and efficiency of SEV internalization by the human cervical epithelial cell line, primary peripheral blood lymphocytes, and primary blood-derived monocytes were significantly higher than those of BEVs. Mechanistically, removal of sialic acids from the surface of EVs using neuraminidase treatment significantly decreased SEV’s surface charge, concomitant with a substantial reduction in SEV’s internalization. The neuraminidase effect was independent of HIV infection and insignificant for BEVs. Finally, these results were corroborated by enrichment of glycoproteins in SEVs versus BEVs. Taken together, these findings uncover fundamental tissue-specific differences in surface electrostatic properties of EVs and highlight the critical role of surface charge in EV/target cell interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization

Kaddour

Panzner

Welch

et al. 2020

Viruses

View full text Add to dashboard Cite

show abstract

“…Furthermore, sialic acid may play an important role in the process of fertilization [19]. Several authors reported the sialic acid content in human sperm [ 10,11,161. These authors used the washed sperm prepared by centrifugation of the diluted semen.…”

Section: Introductionmentioning

confidence: 99%

Sialic Acid in Purified Human Sperm

Oshio

Kaneko

Iizuka

et al. 1987

Archives of Andrology

View full text Add to dashboard Cite

Sialic acid levels of human sperm were estimated using newly developed sperm purification procedure (inner column method). This was used to estimate net sialic acid content of forward motile sperm of normal morphology without any contamination of the seminal plasma, other cellular components, and immature or abnormal sperm. The sialic acid content in purified sperm was approximately 40% of that in the washed (dilution-washing method) sperm. It is suggested that biochemical properties of human sperm should be reevaluated using the inner column purification procedure.

show abstract

“…In view of the fact that both human sperm and seminal plasma possess sialic acids (Svenerholm 1958;Warren 1959;Hudson et al 1965;Levinsky et al 1983a,b), the likely occurrence of sialoglycoproteins on the surface of human sperm and in human seminal plasma is anticipated. Since certain of the sialoglycoproteins cited above are involved in sperm maturation, motility or capacitation, we considered it worthwhile to identify the sialoglycoproteins in human ejaculated sperm and seminal plasma.…”

mentioning

confidence: 99%

Sialoglycoproteins of ejaculated human spermatozoa and seminal plasma

Wongkome

Chulavatnatol

1984

Int J of Andrology

View full text Add to dashboard Cite

Ejaculated human sperm were found to possess three major sialoglycoproteins with molecular weights of 30 000, 14 000 and 12 000 and one minor species of 18 000. Liquefied seminal plasma from normal donors contain two major sialoglycoproteins with molecular weights of 17 000 and 15 000 and two minor species of 70 000 and 54 000. In contrast, the liquefied sperm-free semen of vasectomized men had two major species of sialoglycoproteins of 20 000 and 18 000 daltons plus two minor species of 54 000 and 40 000.

show abstract

Sialic Acid in Semen, Spermatozoa and Serum of Mammals

Cited by 9 publications

References 10 publications

Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization

Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization

Sialic Acid in Purified Human Sperm

Sialoglycoproteins of ejaculated human spermatozoa and seminal plasma

Contact Info

Product

Resources

About