Sialic Acids

Blix, Gunnar; LINDBERG, E.; Odin, Lars; Werner, Ivar

doi:10.1038/175340a0

Cited by 88 publications

(50 citation statements)

References 6 publications

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“…Recently lhillard (23) has confirmed these results and also isolated the NAGal(1-3) 6 2 Sialyl t released &Iic wid as crystalline NANA, the 0-acetyl group being c : d y lost during preparation procedures. Although Faillard, misled by about lOyo too low nitrogen :tnalyses, still assigned the incorrect formula C1&lOloK to NANA, there can be little doubt of the identity of his compound.…”

Section: Identification Of Split Product As Acetylated Neuraminicsupporting

confidence: 51%

Neuraminidase: Its Substrate and Mode of Action

Gottschalk¹

1959

Advances in Enzymology - And Related Areas of Molecular Biology

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Section: Identification Of Split Product As Acetylated Neuraminicsupporting

confidence: 51%

Neuraminidase: Its Substrate and Mode of Action

Gottschalk¹

1959

Advances in Enzymology - And Related Areas of Molecular Biology

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“…That the label is overwhelmingly incorporated into analogues of sialic acid can also be demonstrated electrophoretically (Fig. 1), since mild acid hydrolysis (0.1 M; 80°C; 1 h) of solubilized preparations almost completely removes the label from (glyco)protein acceptors under conditions specific for removal of sialic acid residues (Blix et al, 1956). The residual peak running close to the marker dye is absent when the non-diffusible material is rerun after dialysis and can be attributed to the released analogues of sialic acid.…”

Section: Radioactive Labellingmentioning

confidence: 98%

Rapid isolation of plasma membranes in high yield from cultured fibroblasts

Thom¹,

Powell²,

Lloyd³

et al. 1977

Biochemical Journal

296

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1. A method was developed which allows the rapid preparation of pure plasma membranes in high yield from cultured fibroblasts. 2. Cells are lysed in hypo-osmotic borate/EDTA and, after differential centrifugation, the membranes collected by centrifugation on a sucrose barrier. 3. Electron microscopy of the isolated material shows large membrane vesicles essentially free from contaminating organelles. 4. There is no detectable activity of the endoplasmic-reticulum enzyme marker, NADH2—lipoamide oxidoreductase (EC 1.6.4.3), and that of succinate dehydrogenase (EC 1.3.99.1), a marker for mitochondria, is substantially decreased. Chemical compositions are in good agreement with previous observations. 5. This study confirms the usefulness of applied isotopic markers for isolating plasma membranes.

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“…On the other hand, protein glycosylation has several roles in cell-cell interaction, cell-matrix interaction, communication and cellular signaling (6 -8). A nine carbon sugar unit termed sialic acid (SA) can be bound to the nonreducing ends of glycans attached to certain membrane proteins, secreted proteins and lipids (9). SA is involved in many physiological processes such as cell-cell interaction and molecular recognition, and is important in different pathophysiological process, including brain development and cancer metastasis (10 -14).…”

Section: Pluripotent Embryonic Stem Cell (Esc)mentioning

confidence: 99%

Comprehensive Quantitative Comparison of the Membrane Proteome, Phosphoproteome, and Sialiome of Human Embryonic and Neural Stem Cells

Melo‐Braga

Schulz

Liu

et al. 2014

Molecular & Cellular Proteomics

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Human embryonic stem cells (hESCs) can differentiate into neural stem cells (NSCs), which can further be differentiated into neurons and glia cells. Therefore, these cells have huge potential as source for treatment of neurological diseases. Membrane-associated proteins are very important in cellular signaling and recognition, and their function and activity are frequently regulated by posttranslational modifications such as phosphorylation and glycosylation. To obtain information about membraneassociated proteins and their modified amino acids potentially involved in changes of hESCs and NSCs as well as to investigate potential new markers for these two cell stages, we performed large-scale quantitative membrane-proteomic of hESCs and NSCs. This approach employed membrane purification followed by peptide dimethyl labeling and peptide enrichment to study the membrane subproteome as well as changes in phosphorylation and sialylation between hESCs and NSCs. Combining proteomics and modification specific proteomics we identified a total of 5105 proteins whereof 57% contained transmembrane domains or signal peptides. The enrichment strategy yielded a total of 10,087 phosphorylated peptides in which 78% of phosphopeptides were identified with >99% confidence in site assignment and 1810 unique formerly sialylated N-linked glycopeptides. Several proteins were identified as significantly regulated in hESCs and NSC, including proteins involved in the early embryonic and neural development. In the latter group of proteins, we could identify potential NSC markers as Pluripotent embryonic stem cell (ESC)1 -derived neural stem cells (NSCs) can differentiate into neurons and glia cells of the central nervous system (1), including specialized neuron types like dopaminergic, representing a potential source for treatment of neurological diseases, such as ParkinsonЈs disease. Therefore, a better understanding of the cellular processes behind the changes of hESCs into NSCs, including solid markers for each cell type, is fundamental to move forward with a successful regenerative cell therapy and to investigate the early human neurogenesis processes.Many markers have been reported for the two types of stem cells (2, 3), however several of these markers are also identified in other stem or progenitor cells such as CD133 (Prominin-1) (4). Discovery of cell surface specific markers for differentiated stem cells is highly relevant for future clinical applications. In particular being able to distinguish the developmental stages of the differentiation from parental stem cells to fully mature cells would allow a correct manipulation and isolation of the cell type of interest. Moreover, such study 1 The abbreviations used are: CID, collision-induced dissociation; FDR, false discovery rate; FLR, false localization rate; HCD, highenergy collision induced dissociation; hESC, human embryonic stem cell; LC-MS/MS, liquid chromatography-tandem mass spectrometry; MSA, multistage activation; NSC, neural stem cell; PTM, post-translational modificat...

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Sialic Acids

Cited by 88 publications

References 6 publications

Neuraminidase: Its Substrate and Mode of Action

Neuraminidase: Its Substrate and Mode of Action

Rapid isolation of plasma membranes in high yield from cultured fibroblasts

Comprehensive Quantitative Comparison of the Membrane Proteome, Phosphoproteome, and Sialiome of Human Embryonic and Neural Stem Cells

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