SIAMESE-RELATED1 Is Regulated Posttranslationally and Participates in Repression of Leaf Growth under Moderate Drought

Dubois, Marieke; Selden, Katia; Bédiée, Alexis; Rolland, Gaëlle; Baumberger, Nicolas; Noir, Sandra; Bach, Liên; Lamy, Geneviève; Granier, Christine; Genschik, Pascal

doi:10.1104/pp.17.01712

Cited by 36 publications

(48 citation statements)

References 76 publications

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“…However, mutation of three highly conserved residues at the N terminus of motif A did not disrupt the interaction with CDKA, and it was only when all 10 residues of motif A were substituted by Ala that the interaction was disrupted. The recent results of Dubois et al (2018) indicate that a C-terminal region of SMR1/LGO lacking motifs A and B also may be involved in binding to CDKA;1. Perhaps multiple regions of SIM contribute to its binding to CDK.…”

Section: Motif a In Sim Is A Potential Novel Cdk Interaction Motifmentioning

confidence: 99%

“…SMR1/LGO regulates endoreplication in giant cells of the sepal epidermis as well as during leaf development (Roeder et al, 2010), and SMR2 plays a role in the timing of the switch from cell division to endoreplication early in leaf development (Kumar et al, 2015), emphasizing the role of SMRs in controlling endoreplication. SMRs also have been implicated in blocking cell division in response to DNA damage or drought stress and in responses to pathogens (Wang et al, 2014;Yi et al, 2014;Hamdoun et al, 2016;Schwarz and Roeder, 2016;Dubois et al, 2018). Multiple SMRs from Arabidopsis, as well as other plants, can complement the sim trichome phenotype, in which cells divide several times instead of endoreplicating their DNA, indicating that there is an underlying functional similarity among all members of the family tested (Peres et al, 2007;Kumar et al, 2015).…”

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confidence: 99%

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Functional Analysis of Short Linear Motifs in the Plant Cyclin-Dependent Kinase Inhibitor SIAMESE

et al. 2018

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Endoreplication, a modified cell cycle in which DNA is replicated without subsequent cell division, plays an important but poorly understood role in plant growth and in plant responses to biotic and abiotic stress. The Arabidopsis () () gene encodes the first identified member of the SIAMESE-RELATED (SMR) family of cyclin-dependent kinase inhibitors. controls endoreplication during trichome development, and mutant trichomes divide several times instead of endoreplicating their DNA. The SMR family is defined by several short linear amino acid sequence motifs of largely unknown function, and family members have little sequence similarity to any known protein functional domains. Here, we investigated the roles of the conserved motifs in site-directed Arabidopsis mutants using several functional assays. We identified a potential cyclin-dependent kinase (CDK)-binding site, which bears no resemblance to other known CDK interaction motifs. We also identified a potential site of phosphorylation and two redundant nuclear localization sequences. Surprisingly, the only motif with similarity to the other family of plant CDK inhibitors, the INHIBITOR/INTERACTOR OF CDC2 KINASE/KIP-RELATED PROTEIN proteins, is not required for function in vivo. Because even highly divergent members of the family are able to replace function in Arabidopsis trichomes, it is likely that the results obtained here for will apply to other members of this plant-specific family of CDK inhibitors.

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Section: Motif a In Sim Is A Potential Novel Cdk Interaction Motifmentioning

confidence: 99%

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confidence: 99%

Functional Analysis of Short Linear Motifs in the Plant Cyclin-Dependent Kinase Inhibitor SIAMESE

et al. 2018

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“…It is by now clear that SIM can bind to and inhibit both CDKA;1 and CDKB;1 complexes, in vitro, and that binding to CDKs requires sequences in Motif A (Dubois et al, 2018; Kumar et al, 2018; Kumar et al, 2015; Van Leene et al, 2010). However, there is conflicting evidence about which cyclins SIM might bind to (Churchman et al, 2006; Van Leene et al, 2010), and no previous information on which sequences in SIM are involved in binding to cyclins.…”

Section: Discussionmentioning

confidence: 99%

“…Dubois et al (2018) have found that a threonine residue in a potential CDK phosphorylation site near the N-terminus of SMR1, the SMR most closely related to SIM, may play a role in ubiquitin-mediated proteolysis of SMR1. They have proposed that phosphorylation of this residue by CDKA;1 targets SMR1 for degradation.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, an affinity-tagging proteomics study indicated that while most SMRs bound CYCD and CDKA;1, SIM and the closely related SMR1 protein bound to a CYCB and CDKB1;1, and not to CYCDs or CDKA;1 (Van Leene et al, 2010). More recently, interaction was detected between SIM and both CDKA;1 and CDKB1;1 in Arabidopsis protoplasts (Kumar et al, 2015), and another study found interaction of SMR1 with both CDKA;1 and CDKB1;1 in pulldown experiments from transgenic plant extracts (Dubois et al, 2018). Furthermore, genetic and biochemical studies show that both CYCD3 function and CDKB1 function are necessary for cell division in sim mutant trichomes, and that SIM can inhibit the kinase activity of both CDKA;1 and CDKB1;1 in vitro (Kumar et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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The CDK inhibitor SIAMESE targets both CDKA;1 and CDKB1 complexes to establish endoreplication in trichomes

Larkin

Wang

Zeringue

et al. 2020

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One sentence summary:The cyclin-dependent kinase inhibitor SIAMESE (SIM) 9 targets both CDKA;1 and CDKB1 complexes to establish endoreplication, and that SIM 10 interacts with the cyclin CYCA2;3 via SIM Motif A. Abstract 25 Endoreplication, also known as endoreduplication, is a modified cell cycle in 26 which DNA is replicated without subsequent cell division. Endoreplication plays 27 important roles in both normal plant development and in stress responses. The 28 SIAMESE (SIM) gene of Arabidopsis (Arabidopsis thaliana) encodes a cyclin-dependent 29 kinase inhibitor that plays an central role in establishing endoreplication, and is the 30 founding member of the SIAMESE-RELATED (SMR) family of plant-specific cyclin-31 dependent kinase inhibitors genes. However, there has been conflicting evidence 32 regarding which specific cyclin/CDK complexes are inhibited by SIM in vivo. In this 33 work, we use genetic evidence to show that SIM likely inhibits both CDKA;1-and 34 CDKB1-containing CDK complexes in vivo to promote endoreplication in developing 35Arabidopsis trichomes. We also show that SIM interacts with CYCA2;3, a binding 36 partner of CDKB1;1, via SIM Motif A, which we previously identified as a CDK-binding 37 motif. In contrast, SIM Motif C, which has been indicated as a cyclin binding motif in 38 other contexts, appears to be relatively unimportant for interaction between SIM and 39 CYCA2;3. Together with earlier results, our work suggests that SIM and other SMRs 40 likely have a multivalent interaction with CYC/CDK complexes. 41 42 43Classically, the eukaryotic cell cycle is divided into four phases: G1, S, G2 and 44 M, which are followed by cytokinesis. Cell cycle regulation depends in large part on 45 specific cyclin and cyclin-dependent kinase (CDK) complexes that regulate both the 46 G1→S and the G2→M transitions, which are the two major cell cycle checkpoints 47

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Thriving under Stress: How Plants Balance Growth and the Stress Response

2020

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SIAMESE-RELATED1 Is Regulated Posttranslationally and Participates in Repression of Leaf Growth under Moderate Drought

Cited by 36 publications

References 76 publications

Functional Analysis of Short Linear Motifs in the Plant Cyclin-Dependent Kinase Inhibitor SIAMESE

Functional Analysis of Short Linear Motifs in the Plant Cyclin-Dependent Kinase Inhibitor SIAMESE

The CDK inhibitor SIAMESE targets both CDKA;1 and CDKB1 complexes to establish endoreplication in trichomes

Thriving under Stress: How Plants Balance Growth and the Stress Response

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