SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis

Yao, Wenjie; Shi, Jian; Jiang, Ling; Guo, Yadong; Ding, Chensen; Ding, Yanjun

doi:10.1007/s00604-020-04378-5

Cited by 14 publications

(20 citation statements)

References 29 publications

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“…They applied ISM on five aptamers initially selected by cell-SELEX, increasing the affinity in 36%, leading to the conclusion that the base guanine plays a key role in the aptamer binding capacity across the whole bacteria cell. Later, using this previous described aptamer for P. mirabilis [19], Yao et al, 2020 [20], developed a new fluorescent semiconductor nanocrystal for the detection of P. mirabilis in blood, urine, and milk. The aptasensor proved to be stable and easily synthesized when tested on milk and simulated forensic blood and urine samples.…”

Section: Resultsmentioning

confidence: 99%

Application of aptamer technology in enterobacteria and non-fermenters: literature review

Bes

Farrel

Santos³

et al. 2022

Preprint

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Antimicrobial resistance has grown exponentially in the last decade and become a global health threat. The antibiotic resistance crisis has guided the scientific community to explore non-conventional interventions to target resistant bacteria. Development of new technologies, such as aptamers-based treatment and diagnosis, has shown to be promising with remarkable advantages over the past five years. This narrative review aims on what is already known regarding application of aptamer technology in enterobacteria and non-fermenters, and the prospects for future achievements. A systematic search of the English literature was performed on the 7th of December 2021 to identify papers on aptamer discovery, with a focus on gram negative isolates, published from January 01, 1993, to December 07, 2021, under the topics: (aptamer OR aptamers OR SELEX) AND (bacteria OR sepsis OR non-fermenter OR Enterobacteriaceae OR infection)). The reference lists of included articles were also searched, in addition to hand-searching of various relevant high-impact journals. Out of 2,474 articles, 30 experimental studies were recruited for review, and are chronologically described. Although the number of publications regarding development of aptamers to target these pathogenic agents has increased over the years, the recent publications are mostly around diagnostic devices manufactured using previously described aptamers. There have been less than one-third of the studies describing new and specific aptamers. From the 30 selected papers, 18 are regarding non-fermenters, seven approaching multi-species of bacteria and only five regarding a single enterobacteria. Even for the newly described aptamers, most of the published papers pertain to diagnostic aptamers and only seven focus on aptamers for therapeutics. The number of aptamers with strong and specific binding capacity are still limited. Improving the current SELEX and developing more APT remains the major hurdle for aptamer related studies.

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Section: Resultsmentioning

confidence: 99%

Application of aptamer technology in enterobacteria and non-fermenters: literature review

Bes

Farrel

Santos³

et al. 2022

Preprint

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“…Aptamers are employed in biosensing devices as probes. The specificity of aptamers as molecular recognition element makes aptamer-based sensors (aptasensors) uniquely suited for selective detection of targets in blood, food samples, or pathogens [75]. They are superior to conventional biosensors that use antibodies as they are stable, have high affinity to targets, modifiable, and can be developed for a wide range of targets, using various transduction mechanisms.…”

Section: Aptamers In Diagnosticsmentioning

confidence: 99%

Recent Progress and Opportunities for Nucleic Acid Aptamers

Byun

2021

Life

102

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Coined three decades ago, the term aptamer and directed evolution have now reached their maturity. The concept that nucleic acid could modulate the activity of target protein as ligand emerged from basic science studies of viruses. Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding, which allow for therapeutic and diagnostic applications. Compared to traditional antibodies, aptamers have several advantages, including small size, flexible structure, good biocompatibility, and low immunogenicity. In vitro selection method is used to isolate aptamers that are specific for a desired target from a randomized oligonucleotide library. The first aptamer drug, Macugen, was approved by FDA in 2004, which was accompanied by many studies and clinical investigations on various targets and diseases. Despite much promise, most aptamers have failed to meet the requisite safety and efficacy standards in human clinical trials. Amid these setbacks, the emergence of novel technologies and recent advances in aptamer and systematic evolution of ligands by exponential enrichment (SELEX) design are fueling hope in this field. The unique properties of aptamer are gaining renewed interest in an era of COVID-19. The binding performance of an aptamer and reproducibility are still the key issues in tackling current hurdles in clinical translation. A thorough analysis of the aptamer binding under varying conditions and the conformational dynamics is warranted. Here, the challenges and opportunities of aptamers are reviewed with recent progress.

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“…Besides, the dispersibility was spectacular, which can maintain more than seven months in the air with the pristine luminescent properties [84]. Moreover, the long fluorescence lifetime of ultrasmall size SiC QDs has touched 2.59 ns [86]. Yao et al used aptamer-modified SiC quantum dots as a fluorescent aptamer sensor for the detection of Proteus mirabilis [87].…”

Section: Sic Dotsmentioning

confidence: 99%

Two-dimensional quantum dots for biological applications

Niu

Gao

et al. 2021

Nano Res.

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The two-dimensional quantum dots (2D-QDs) have been developed significantly in the past decades. The 2D-QDs could be used in bioimaging, biosensing, drug/gene delivery, and photodynamic/photothermal therapy. The potential applications in biology receive increasing attention, which makes them the novel and emerging candidates in biomaterial research fields. In this context, we discuss a variety of 2D-QDs with different physical and chemical properties. We focuse on the latest synthesis progress and recent applications in biotechnological, and biomedical applications of the 2D-QDs and we also evaluate the challenges and prospects in this field.

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SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis

Cited by 14 publications

References 29 publications

Application of aptamer technology in enterobacteria and non-fermenters: literature review

Application of aptamer technology in enterobacteria and non-fermenters: literature review

Recent Progress and Opportunities for Nucleic Acid Aptamers

Two-dimensional quantum dots for biological applications

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