Siccibacter turicensis from Kangaroo Scats: Possible Implication in Cellulose Digestion

Dhakal, Sudip; Boath, Jarryd M.; Van, Thi Thu Hao; Moore, Robert J.; Macreadie, Ian

doi:10.3390/microorganisms8050635

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…According to the correlation analysis in this study, it was speculated that Siccibacter was probably linked to decreased DM loss, and similar to Lactobacillus , it might have the powerful ability to lower pH in ML silage than Carnobacterium , in spite of its small population in entire microflora. As reported, Siccibacter turicensis played beneficial roles in the cellulases, such as the endoglucanase ( Dhakal et al, 2020 ), and thus, Siccibacter could potentially be exploited as a probiotic microbe for ML silage. For nutrient substances, Enterobacter correlated with decreased amino acid and total flavonoids contents.…”

Section: Resultsmentioning

confidence: 93%

Optimized Ensiling Conditions and Microbial Community in Mulberry Leaves Silage With Inoculants

Cui

Yang²,

Zhang³

et al. 2022

Front. Microbiol.

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Mulberry leaves (ML) are a promising alternative fodder source due to their high protein content and the abundance of active components. A test of three inoculants in various combinations revealed that high-quality ML silage was produced at an inoculum ratio of 1:1:0 (50% Saccharomyces cerevisiae, 50% Lactobacillus plantarum, and 0% Bacillus subtilis). Using dry matter (DM) loss, pH, ammonia-N and amino acid contents, total antioxidant activity, and total flavonoids content to evaluate silage quality, this inoculant mixture was shown to produce high-quality silage within a range of inoculum size (5–15%), moisture contents (50–67%), ensiling temperatures (27–30°C), and ensiling duration (14–30 days). A third trial comparing silages produced after 30 days at 28°C and 50% moisture content revealed that silage E, prepared using an L. plantarum inoculant alone, displayed the lowest DM loss and pH, and low bacterial diversity, and it was dominated by Lactobacillus (88.6%), with low abundance of Enterobacter (6.17%). In contrast, silage B5, prepared with equal ratios of L. plantarum and S. cerevisiae, was dominated by Enterococcus (67.16%) and Lactobacillus (26.94%), with less marked yeast persistence, and reducing the DM content from 50 to 40% altered these relative abundances to 5.47 and 60.61, respectively. Control silages produced without an inoculant had the highest pH and ammonia-N content (indicative of poor quality), had the lowest antioxidant activity, had higher bacterial diversity, and were dominated by Carnobacterium (74.28%) and Enterococcus (17.3%). In summary, ensiling of ML conditions with proper inoculants yielded high-quality silage with a favorable microbial community composition.

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Section: Resultsmentioning

confidence: 93%

Optimized Ensiling Conditions and Microbial Community in Mulberry Leaves Silage With Inoculants

Cui

Yang²,

Zhang³

et al. 2022

Front. Microbiol.

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“… Siccibacter turicensis ) and Ruminococcus (e.g. Ruminococcus flavefaciens ) can degrade cellulose and enhance herbivores’ survival in harsh environments [60, 62]. Also, Escherichia genus (e.g.…”

Section: Discussionmentioning

confidence: 99%

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

et al. 2021

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Arid plant communities provide variable diets that can affect digestive microbial communities of free-foraging ruminants. Thus, we used next-generation sequencing of 16S and 18S rDNA to characterize microbial communities in the rumen (regurgitated digesta) and large intestine (faeces) and diet composition of lactating creole goats from five flocks grazing in native plant communities in the Sonoran Desert in the rainy season. The bacterial communities in the rumen and large intestine of the five flocks had similar alpha diversity (Chao1, Shannon, and Simpson indices). However, bacterial community compositions were different: a bacterial community dominated by Proteobacteria in the rumen transitioned to a community dominated by Firmicutes in the large intestine. Bacterial communities of rumen were similar across flocks; similarly occurred with large-intestine communities. Archaea had a minimum presence in the goat digestive tract. We detected phylum Basidiomycota, Ascomycota, and Apicomplexa as the main fungi and protozoa. Analyses suggested different diet compositions; forbs and grasses composed the bulk of plants in the rumen and forbs and shrubs in faeces. Therefore, lactating goats consuming different diets in the Sonoran Desert in the rainy season share a similar core bacterial community in the rumen and another in the large intestine and present low archaeal communities.

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“…Y. regensburgei has recently been identified as a novel pathogen in farmed American alligators (Alligator mississippiensis), causing acute mortality secondary to pneumonia and septicaemia. 30 The 16S rRNA gene sequence of Y. regensburgei is closely related to the bacteria Siccibacter turicensis, a normal component of the wild kangaroo gut microbiota responsible for cellulose degradation 31 ; however, while other members of the Enterbacteriaceae family have been routinely identified in the hindgut and pouch of the koala, and are able to withstand high concentrations of eucalyptus oil compared with other enteric bacteria, 32 Y. regensburgei has not previously been cultured and is unlikely to be a normal commensal organism in this species (personal communications: Professor I Macreadie via email 23 June 2020; Professor K Belov via email 26 June 2020). To the authors' knowledge, this report of Y. regensburgei otitis media combined with otitis externa in a koala represents the first clinical infection described in the literature outside of humans and alligators.…”

Section: Discussionmentioning

confidence: 99%

Refractory otitis media and otitis externa in a koala (Phascolarctos cinereus) treated with total ear canal ablation and lateral bulla osteotomy

Swaffield,

Liberts,

Gillett

et al. 2023

Aust Veterinary J

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BackgroundTo the authors' knowledge, this is the first report fully describing the surgical and medical management of otitis media and otitis externa in the koala (Phascolarctos cinereus) treated by total ear canal ablation and lateral bulla osteotomy.Case ReportAn adult male koala captured as part of a monitoring project in South East Queensland was diagnosed with chlamydial cystitis. Purulent discharge from the right ear was also observed; diagnostic imaging was consistent with otitis media and otitis externa. Yokenella regensburgei was repeatedly cultured from the site. Clinical signs resolved with topical antibiotic therapy; however, recrudesced following cessation of antibiotics. A total ear canal ablation and lateral bulla osteotomy was performed, followed by an extended period of systemic antibiotic therapy. Mild facial nerve paresis was observed for 4 weeks postoperatively and resolved spontaneously. The koala remained clinically healthy for the 6 months it was monitored following release.ConclusionTotal ear canal ablation combined with lateral bulla osteotomy appears to be an appropriate surgical intervention for otitis media combined with otitis externa refractory to medical management in the koala. Transient postoperative facial nerve paresis is a possible complication, as documented in other species. To the authors' knowledge this is the first case of Yokenella regensburgei infection outside of humans and American alligators (Alligator mississippiensis); the clinical significance of this pathogen in the koala remains unknown.

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Siccibacter turicensis from Kangaroo Scats: Possible Implication in Cellulose Digestion

Cited by 7 publications

References 30 publications

Optimized Ensiling Conditions and Microbial Community in Mulberry Leaves Silage With Inoculants

Optimized Ensiling Conditions and Microbial Community in Mulberry Leaves Silage With Inoculants

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

Refractory otitis media and otitis externa in a koala (Phascolarctos cinereus) treated with total ear canal ablation and lateral bulla osteotomy

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