Sicwinv1, a Cell Wall Invertase from Sesame, Is Involved in Anther Development

Zhou, Ting; Hao, Ge‐Fei; Yang, Yuanxiao; Liu, Hongyan; Yang, Ming; Zhao, Yingzhong

doi:10.1007/s00344-019-09932-x

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…As revealed in Fig. 2A, CWINV1 (N09, catalysing the hydrolysis of sucrose into glucose and fructose) (Goetz et al, 2017; Zhou et al, 2019) was expressed at higher levels in the 3‐day‐ and 6‐day‐UP pistils, while its expression in the 0‐day‐UP pistil was minimal. In the pollinated pistils, CWINV1 was expressed at higher levels in the 6‐day‐P styles compared to other style ages, but its expression levels were lower in pollinated than unpollinated pistils (Fig.…”

Section: Resultsmentioning

confidence: 95%

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

et al. 2020

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Delayed pollination is widely used to overcome pre‐fertilization incompatibility, but its regulatory mechanisms are unclear. When Nicotiana tabacum was cross‐pollinated with pollen of N. alata, the incompatibility occurring in the basal 1/4 region of the style (pollinated at anthesis: 0‐day‐old pistil) was overcome by delayed pollination (of 6‐day‐old pistil), and the morphological changes and corresponding physiological basis are explored here. The structure and ultrastructure of the pistil were observed under fluorescence microscopy and transmission electron microscopy. Differentially expressed proteins were screened with a monoclonal antibody chip for Nicotiana, and protein expression and distribution were analysed by immunofluorescence. Cellulase and pectinase activities were tested using enzyme‐linked immunosorbent assay kits. The style of Nicotiana is solid in the basal region and pollen tubes grow in the extracellular spaces (ECM) of the transmitting tissue (TTS) cells. Seven of the 22 identified proteins were cell wall‐associated proteins and were expressed at higher levels during pistil senescence. Cellulase and pectinase activities increased. The TTS cells in the basal 1/4 region of the 0‐day‐old style were polygonal and tightly arranged, with narrow ECM, but these were oval or partially dissolved in the 6‐day‐old pistil, leading to wider ECM and richer secretions. The increased expression of cell wall proteins and enhanced enzyme activity during pistil senescence might partially be responsible for the cells becoming oval and the ECM enlarged, providing the morphological basis for delayed pollination overcoming the pre‐fertilization incompatibility between N. tabacum and N. alata.

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Section: Resultsmentioning

confidence: 95%

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

et al. 2020

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“…6b) found in the promoter of TaCWINV40 con rmed its response to low temperature, and the existence of two cis-elements required for pollen speci c expression (GTGANTG10, POLLEN1LELAT52) predicted its expression site. In Arabidopsis, promoter analysis also con rmed the anther-speci c expression pattern of sesame Sicwinv1, and the GUS staining was mainly detected in the anther tapetum (Zhou et al 2019). The tapetum layer is the innermost layer of the microspore mother cell wall (epidermal layer, endothelial layer, middle layer and tapetum layer) after the anther morphogenesis of higher plants is completed, and its degradation at the right time is a necessary condition for the normal development of pollen (Mascarenhas 1989;Yi et al 2016).…”

Section: Inv Gene Family Responds To Temperature Stressmentioning

confidence: 85%

New Insights Into Invertase Gene Family and Functional Characterization of Critical Cell Wall Invertase TaCWINV40 For Male Fertility in Wheat (Triticum Aestivum L.)

et al. 2021

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Invertase (INV, ec3.2.1.26) irreversibly hydrolyzes sucrose into fructose and glucose, and it is regulated by the environment to affect pollen fertility in some plant species. However, there has been a lack of systematic identification of INV gene family in wheat. In order to reveal the potential influence on the male fertility, a total of 130 wheat INVs that unevenly distributed on 21 chromosomes were systematically identified and analyzed in this study. According to physical and chemical properties, subcellular location, and phylogenetic tree, they were divided into two acidic INV (AINV) subtypes: cell wall group (TaCWINV1-68), vacuole group (TaVINV1-42), and two neutral/alkaline INV (A/NINV) subtypes: cytoplasmic α group (TaA/NINV1-11) and cytoplasmic β group (TaA/NINV12-20). The amplification of A/NINVs is mainly attributed to the polyploidization of wheat, and the multiple duplication events experienced in AINVs revealed their non-dose sensitivity characteristic. The wheat RNA-seq data revealed the tissue specificity of A/NINVs and AINVs, and six spike-specific CWINVs showed significant differential expression between the fertile and sterile anthers of thermo-sensitive male-sterile wheat KTM3315A. TaCWINV40 localized in cell wall was effectively silenced in the fertile KTM3315A, and the malformed pollen grains and non-germinating pollen tubes shed light on its indispensability in the development of wheat anthers. This study will spur the interest on manipulating the novel genetic characteristics of TaCWINVs for the construction and improvement of wheat male sterile materials.

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“…However, polysaccharides biosynthesis at the molecular level in D. o cinale remains unclear, especially related to gene function and transcription regulation. As an essential enzyme for polysaccharides synthesis, INV promotes the accumulation of polysaccharides through sucrose hydrolysis, which moderates various biological processes such as growth and development [19,20],…”

Section: Introductionmentioning

confidence: 99%

Genomic Identification and Expression Analysis of Acid Invertase (AINV) Gene Family in Dendrobium officinale Kimura et Migo

Liu

Luo

et al. 2023

Preprint

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Background Dendrobium officinale Kimura et Migo, a traditional Chinese orchid herb famous for its great horticultural and medicinal value, grows in an adverse habitat and suffers from abiotic or biotic stresses. Acid invertases (AINVs) are widely considered enzymes involved in regulating sucrose metabolism and here revealed to participate in responses to environmental stress in plants. The AINV gene family members have been identified and characterized in multiple plant genomes. However, detailed information about this gene family and their expression patterns are unknown in D. officinale despite their significance in polysaccharide biosynthesis. Results This study systematically analyzed the D. officinale genome and identified four DoAINV genes, classified into two subfamilies based on subcellular prediction and phylogenetic analysis. Comparison of gene structures and conserved motifs in DoAINV genes showed high conservation during their evolution. The conserved amino acids and domains of DoAINV proteins were characterized as pivotal for their function. Abiotic and biotic stress responses cis-elements were the most abundant motif in all DoAIVN genes, indicating they were stress-responsive. Furthermore, bioinformatics analysis of transcriptome data, validated by quantitative real-time reverse transcription PCR (qRT-PCR), revealed distinct organ-specific expression patterns of DoAINV genes in eight tissues and in response to abiotic stresses. Soluble sugar content and interaction network were examined to provide information on stress release and sucrose metabolism. Conclusions DoAINV genes are involved in various activities such as growth and development, stress response, and polysaccharide biosynthesis. The results provide valuable insights into the AINV family and will be helpful in further investigating DoAINV functions in D. officinale.

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Sicwinv1, a Cell Wall Invertase from Sesame, Is Involved in Anther Development

Cited by 11 publications

References 35 publications

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

New Insights Into Invertase Gene Family and Functional Characterization of Critical Cell Wall Invertase TaCWINV40 For Male Fertility in Wheat (Triticum Aestivum L.)

Genomic Identification and Expression Analysis of Acid Invertase (AINV) Gene Family in Dendrobium officinale Kimura et Migo

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