SID-I is implicated in systemic gene silencing in the honey bee

Aronstein, Katherine A.; Pankiw, Tanya; Saldivar, Eduardo

doi:10.1080/00218839.2006.11101307

Cited by 96 publications

(105 citation statements)

References 13 publications

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“…Delivery of dsRNA by feeding has shown that concentrations as low as 7 μg/ml for the honey bee Toll-related receptor 18 W gene (Aronstein et al, 2006) and as high as 450 μg/ml for the phosphatase and tensin (PTEN) homolog fed for four consecutive days (Mutti et al, 2011) are necessary to achieve gene knockdown. Our results and previous studies indicate that each gene performs differently in honey bees and supports the idea that dsRNAs for different genes should be evaluated independently.…”

Section: Discussionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

et al. 2016

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“…Some of them manipulated eggs or applied dsRNA to larvae either by feeding or injections (Aronstein and Saldivar 2005;Beye et al 2003;Aronstein et al 2006;Patel et al 2007;Kucharski et al 2008;Nunes and Simões 2009;Maori et al 2009), whereas there are only very few reports on the successful manipulation of adult individuals (Amdam et al 2003;Farooqui et al 2004;Seehuus et al 2006;Schlüns and Crozier 2007;Gatehouse et al 2004;Müßig et al 2010;Mustard et al 2010). …”

Section: Introductionmentioning

confidence: 99%

“…It is involved in dsRNA internalization in C. elegans and humans and facilitates systemic RNAi responses (Winston et al 2002;Feinberg and Hunter 2003). This gene is particularly suited because Aronstein et al (2006) report on a correlation between amSID-1 expression and the application of dsRNA in adult honeybees.…”

Section: Introductionmentioning

confidence: 99%

RNA interference in honeybees: off-target effects caused by dsRNA

Jarosch

Moritz

2011

Apidologie

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RNA interference involves the targeted knockdown of mRNA triggered by complementary dsRNA molecules applied to an experimental organism. Although this technique has been successfully used in honeybees (Apis mellifera), it remains unclear whether the application of dsRNA leads to unintended expression knockdown in unspecific, non-targeted genes. Therefore, we studied the gene expression of four non-target genes coding for proteins that are involved in different physiological processes after treatment with three dsRNAs in two abdominal tissues. We found unspecific gene downregulation depending on both the dsRNA used and the different tissues. Hence, RNAi experiments in the honeybee require rigid controls and carefully selected dsRNA sequences to avoid misinterpretation of RNAi-derived phenotypes.

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“…Evidence in multiple systems, including the honey bee, suggests that selective transport and amplification of small RNA exists. [60][61][62] Thus, specific miRNA species not examined here might be taken up more efficiently into tissues. In addition, specialized populations within a given tissue might possess mechanisms for efficient uptake that would result in high levels of miRNA in these cells that remains undetectable within the tissue as a whole in our assays.…”

Section: Discussionmentioning

confidence: 99%

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

et al. 2015

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The putative transfer and gene regulatory activities of diet-derived miRNAs in ingesting animals are still debated. Importantly, no study to date has fully examined the role of dietary uptake of miRNA in the honey bee, a critical pollinator in both agricultural and natural ecosystems. After controlled pollen feeding experiments in adult honey bees, we observed that midguts demonstrated robust increases in plant miRNAs after pollen ingestion. However, we found no evidence of biologically relevant delivery of these molecules to proximal or distal tissues of recipient honey bees. Our results, therefore, support the premise that pollen miRNAs ingested as part of a typical diet are not robustly transferred across barrier epithelia of adult honey bees under normal conditions. Key future questions include whether other small RNA species in honey bee diets behave similarly and whether more specialized and specific delivery mechanisms exist for more efficient transport, particularly in the context of stressed barrier epithelia.

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SID-I is implicated in systemic gene silencing in the honey bee

Cited by 96 publications

References 13 publications

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L

RNA interference in honeybees: off-target effects caused by dsRNA

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

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