Side effects by oral application of atmospheric pressure plasma on the mucosa in mice

Jablonowski, Lukasz; Kocher, Thomas; Schindler, Axel; Müller, Karolina; Dombrowski, Frank; Woedtke, Thomas von; Arnold, Thomas; Lehmann, Antje; Rupf, Stefan; Evert, Matthias; Evert, Katja

doi:10.1371/journal.pone.0215099

Cited by 41 publications

(30 citation statements)

References 30 publications

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“…Indeed, we feel that this is a very important topic that is not comprehensively covered in plasma literature. There are only a few studies that mention the side effects of NTPs [ 78 , 79 , 80 ]. Generally, adverse drug reactions or side effects occur almost daily in healthcare institutions and have to be carefully considered in the optimization of treatment modalities [ 81 , 82 , 83 ].…”

Section: Critical Clinical View On Ntp—potential Side Effects and mentioning

confidence: 99%

Critical Analysis of Non-Thermal Plasma-Driven Modulation of Immune Cells from Clinical Perspective

Smolková

Frtús

Uzhytchak

et al. 2020

IJMS

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The emerged field of non-thermal plasma (NTP) shows great potential in the alteration of cell redox status, which can be utilized as a promising therapeutic implication. In recent years, the NTP field considerably progresses in the modulation of immune cell function leading to promising in vivo results. In fact, understanding the underlying cellular mechanisms triggered by NTP remains incomplete. In order to boost the field closer to real-life clinical applications, there is a need for a critical overview of the current state-of-the-art. In this review, we conduct a critical analysis of the NTP-triggered modulation of immune cells. Importantly, we analyze pitfalls in the field and identify persisting challenges. We show that the identification of misconceptions opens a door to the development of a research strategy to overcome these limitations. Finally, we propose the idea that solving problems highlighted in this review will accelerate the clinical translation of NTP-based treatments.

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Section: Critical Clinical View On Ntp—potential Side Effects and mentioning

confidence: 99%

Critical Analysis of Non-Thermal Plasma-Driven Modulation of Immune Cells from Clinical Perspective

Smolková

Frtús

Uzhytchak

et al. 2020

IJMS

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“…In contrast to systemic therapies, CAP only has a locally limited effect. Other studies also indicate that CAP treatment has fewer systemic side effects than conventional therapies [ 52 , 53 , 54 ]. Another favorable property of CAP is that no resistance to CAP treatment has been observed in previous studies [ 52 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

Haralambiev

Neuffer

Nitsch

et al. 2020

IJMS

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Background: Cold atmospheric plasma (CAP) is increasingly used in the field of oncology. Many of the mechanisms of action of CAP, such as inhibiting proliferation, DNA breakage, or the destruction of cell membrane integrity, have been investigated in many different types of tumors. In this regard, data are available from both in vivo and in vitro studies. Not only the direct treatment of a tumor but also the influence on its blood supply play a decisive role in the success of the therapy and the patient’s further prognosis. Whether the CAP influences this process is unknown, and the first indications in this regard are addressed in this study. Methods: Two different devices, kINPen and MiniJet, were used as CAP sources. Human endothelial cell line HDMEC were treated directly and indirectly with CAP, and growth kinetics were performed. To indicate apoptotic processes, caspase-3/7 assay and TUNEL assay were used. The influence of CAP on cellular metabolism was examined using the MTT and glucose assay. After CAP exposure, tube formation assay was performed to examine the capillary tube formation abilities of HDMEC and their migration was messured in separate assays. To investigate in a possible mutagenic effect of CAP treatment, a hypoxanthine-guanine-phosphoribosyl-transferase assay with non malignant cell (CCL-93) line was performed. Results: The direct CAP treatment of the HDMEC showed a robust growth-inhibiting effect, but the indirect one did not. The MMT assay showed an apparent reduction in cell metabolism in the first 24 h after CAP treatment, which appeared to normalize 48 h and 72 h after CAP application. These results were also confirmed by the glucose assay. The caspase 3/7 assay and TUNEL assay showed a significant increase in apoptotic processes in the HDMEC after CAP treatment. These results were independent of the CAP device. Both the migration and tube formation of HDMEC were significant inhibited after CAP-treatment. No malignant effects could be demonstrated by the CAP treatment on a non-malignant cell line.

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“…Depending on its settings, each device would have several effects on biomedical applications [ 12 ]. Considering the low temperature of application, as well as the acceptable thermal damage to tissues [ 13 , 14 , 15 , 16 ], CAP has been suggested as a promising device, not only for systemic biomedical treatments, but also as a chairside approach [ 2 , 17 ] for the treatment of different oral diseases.…”

Section: Overview Of Plasma Features and Functionsmentioning

confidence: 99%

The Emerging Role of Cold Atmospheric Plasma in Implantology: A Review of the Literature

Hui¹,

Perrotti

Iaculli

et al. 2020

Nanomaterials

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In recent years, cold atmospheric plasma (CAP) technologies have received increasing attention in the field of biomedical applications. The aim of this article is to review the currently available literature to provide an overview of the scientific principles of CAP application, its features, functions, and its applications in systemic and oral diseases, with a specific focus on its potential in implantology. In this narrative review, PubMed, Medline, and Scopus databases were searched using key words like “cold atmospheric plasma”, “argon plasma”, “helium plasma”, “air plasma”, “dental implants”, “implantology”, “peri-implantitis”, “decontamination”. In vitro studies demonstrated CAP’s potential to enhance surface colonization and osteoblast activity and to accelerate mineralization, as well as to determine a clean surface with cell growth comparable to the sterile control on both titanium and zirconia surfaces. The effect of CAP on biofilm removal was revealed in comparative studies to the currently available decontamination modalities (laser, air abrasion, and chlorhexidine). The combination of mechanical treatments and CAP resulted in synergistic antimicrobial effects and surface improvement, indicating that it may play a central role in surface “rejuvenation” and offer a novel approach for the treatment of peri-implantitis. It is noteworthy that the CAP conditioning of implant surfaces leads to an improvement in osseointegration in in vivo animal studies. To the best of our knowledge, this is the first review of the literature providing a summary of the current state of the art of this emerging field in implantology and it could represent a point of reference for basic researchers and clinicians interested in approaching and testing new technologies.

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Side effects by oral application of atmospheric pressure plasma on the mucosa in mice

Cited by 41 publications

References 30 publications

Critical Analysis of Non-Thermal Plasma-Driven Modulation of Immune Cells from Clinical Perspective

Critical Analysis of Non-Thermal Plasma-Driven Modulation of Immune Cells from Clinical Perspective

Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

The Emerging Role of Cold Atmospheric Plasma in Implantology: A Review of the Literature

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