Side effects of treatment with busulfan at high doses in dogs

Abstract

Busulfan is an alkylating agent that is commonly used as a preconditioning drug in the clinical transplantation of hematopoietic stem cells, and is also traditionally used method for chemical sterilization (McCune and Holmberg, 2009;Hur et al., 2017). The side effects of busulfan include bone marrow suppression, pulmonary fibrosis, and skin pigmentation. Previous studies have demonstrated that preconditioning using busulfan is more effective than irradiation during human B cell differentiation (Krivoy et al., 2008;Hayakawa et al., 2009). Additionally, busulfan is most commonly used for chemical sterilization during the transplantation of male germ cells. Busulfan depletes male germ cells and disrupts the junctions between Sertoli cells (Bucci and Meistrich, 1987).Numerous studies have reported that the dose of busulfan necessary for sterilization varies widely among species. The dose of busulfan necessary for sterilization in pigs, monkeys, coyotes, mice, rats and chickens is 40-