Side population cells in the human decidua of early pregnancy exhibit stem/progenitor cell-like characteristics

Guo, Chun; Zhu, Huili; Huang, Wei; Li, Shengfu; Qu, Wenwen; Liu, Yaofang; Tan, Aixiang

doi:10.1016/j.rbmo.2010.07.010

Cited by 6 publications

(7 citation statements)

References 24 publications

(32 reference statements)

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“…The decidua of pregnancy may therefore harbour a subpopulation of undifferentiated MSCs related to eMSCs ( Kyurkchiev et al , 2010 ). Indeed, clonogenic SP cells were identified in the first trimester decidua ( Tsuji et al , 2008 ; Guo et al , 2010 ; Wang et al , 2013 ) comprising 0.03–1.4% of cells, a lower abundance than their endometrial counterparts. SP cells sorted from short-term cultured human decidual cells expressed neither CD31 (endothelial marker) nor CD146 (MSC marker) ( Wang et al , 2013 ), but differentiated into endothelial cells in vitro and induced neovascularization following intramascular injection in a mouse ischaemic hind limb injury model, rescuing the limb ( Wang et al , 2013 ).…”

Section: Stem/progenitor Cells In Endometrial Deciduamentioning

confidence: 99%

Endometrial stem/progenitor cells: the first 10 years

Gargett

Schwab

Deane

2015

Hum. Reprod. Update

365

454

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BACKGROUNDThe existence of stem/progenitor cells in the endometrium was postulated many years ago, but the first functional evidence was only published in 2004. The identification of rare epithelial and stromal populations of clonogenic cells in human endometrium has opened an active area of research on endometrial stem/progenitor cells in the subsequent 10 years.METHODSThe published literature was searched using the PubMed database with the search terms ‘endometrial stem cells and menstrual blood stem cells' until December 2014.RESULTSEndometrial epithelial stem/progenitor cells have been identified as clonogenic cells in human and as label-retaining or CD44+ cells in mouse endometrium, but their characterization has been modest. In contrast, endometrial mesenchymal stem/stromal cells (MSCs) have been well characterized and show similar properties to bone marrow MSCs. Specific markers for their enrichment have been identified, CD146+PDGFRβ+ (platelet-derived growth factor receptor beta) and SUSD2+ (sushi domain containing-2), which detected their perivascular location and likely pericyte identity in endometrial basalis and functionalis vessels. Transcriptomics and secretomics of SUSD2+ cells confirm their perivascular phenotype. Stromal fibroblasts cultured from endometrial tissue or menstrual blood also have some MSC characteristics and demonstrate broad multilineage differentiation potential for mesodermal, endodermal and ectodermal lineages, indicating their plasticity. Side population (SP) cells are a mixed population, although predominantly vascular cells, which exhibit adult stem cell properties, including tissue reconstitution. There is some evidence that bone marrow cells contribute a small population of endometrial epithelial and stromal cells. The discovery of specific markers for endometrial stem/progenitor cells has enabled the examination of their role in endometrial proliferative disorders, including endometriosis, adenomyosis and Asherman's syndrome. Endometrial MSCs (eMSCs) and menstrual blood stromal fibroblasts are an attractive source of MSCs for regenerative medicine because of their relative ease of acquisition with minimal morbidity. Their homologous and non-homologous use as autologous and allogeneic cells for therapeutic purposes is currently being assessed in preclinical animal models of pelvic organ prolapse and phase I/II clinical trials for cardiac failure. eMSCs and stromal fibroblasts also exhibit non-stem cell-associated immunomodulatory and anti-inflammatory properties, further emphasizing their desirable properties for cell-based therapies.CONCLUSIONSMuch has been learnt about endometrial stem/progenitor cells in the 10 years since their discovery, although several unresolved issues remain. These include rationalizing the terminology and diagnostic characteristics used for distinguishing perivascular stem/progenitor cells from stromal fibroblasts, which also have considerable differentiation potential. The hierarchical relationship between clonogenic epithelial progenitor cells, ...

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Section: Stem/progenitor Cells In Endometrial Deciduamentioning

confidence: 99%

Endometrial stem/progenitor cells: the first 10 years

Gargett

Schwab

Deane

2015

Hum. Reprod. Update

365

454

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“…In addition, the decidua is thought to function as an active immune regulation partner for the immune tolerance microenviroment at the maternal-fetal interface. It has been proposed that a population of stem cells exists within the human decidua, which may be responsible for mediating cell proliferation during embryonic implantation and the formation of the placenta (9). The maintenance of pregnancy involves maternal tolerance to the fetal allograft, which is associated with decidual natural killer cell (dNK) tolerance phenotype (10) and a T-helper (Th) 2 cell bias at the maternal-fetal interface (11).…”

Section: Introductionmentioning

confidence: 99%

Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte-associated immunoglobulin-like receptor 1

Man

et al. 2017

Molecular Medicine Reports

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The development of maternal tolerance to the fetal allograft in critical for the maintenance of the pregnancy, and it is accompanied by the development of a special decidual natural killer (dNK) cell tolerance phenotype. To understand the factors that influence dNK cells during early pregnancy, the present study aimed to identify mesenchymal stem cells (MSCs) from human first‑trimester deciduas, termed decidual MSCs (DMSCs), and to investigate the effect of DMSCs on the regulation of dNK cells via collagen. Decidual samples were collected from women with normal pregnancy that had undergone elective vaginal surgical terminations at 6‑9 weeks gestation. DMSCs derived from human decidual tissues were cultured under differentiation conditions to examine their multipotent differentiation capacities, and the expression of MSC‑specific markers, including cluster of differentiation (CD)44, CD73, CD105, CD90, CD34, CD31, CD14, CD45, CD11b and human leukocyte antigen‑antigen D related, was determined. dNK cells were co‑cultured with DMSCs in order to examine the effect of DMSCs on the tolerance phenotype of dNK cells. The expression of cell surface molecules, natural cytotoxicity triggering receptor 3 and killer cell immunoglobulin‑like receptor (KIR) 2DL1, and the secretion of cytokines, including interferon‑γ, tumor necrosis factor (TNF)‑α, interleukin (IL)‑10, IL‑4 and perforin, were examined by flow cytometry analysis. To determine whether the regulation of dNK cells by DMSCs was mediated by collagen, DMSCs were pre‑treated with human recombinant leukocyte‑associated immunoglobulin‑like receptor (LAIR)‑2 and transfected with pScoR‑GFP‑hP4H to inhibit the interaction between LAIR‑1 and collagen. The present results demonstrated that collagen produced by DMSCs increased the expression of KIR2DL1 and IL‑4, decrease the expression of NKp30 and TNF‑α. In conclusion, the results of the present study demonstrated that DMSCs may be cultured in vitro for prolonged periods, whilst retaining the ability to differentiate into different cell lineages. In addition, DMSCs may modulate the function of dNK cells via the interaction between collagen and LAIR‑1.

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“…Our previous work has proved that SP cells from human decidua can proliferate and form colonies during long-time culture in vitro and differentiate into mesenchymal cells [17,18]. …”

Section: Resultsmentioning

confidence: 99%

“…The primary decidua cells from human first-trimester fetuses (n = 8) were separated, cultured for 24 to 48 hours and then digested and labeled with Hoechst 33342 (Invitrogen, Paisley, UK) as previously described [17,18]. Then the cells were incubated with mouse anti-human CD31 (fluorescein isothiocyanate (FITC), BD Biosciences San Jose, CA, USA) and mouse anti-human CD146 (phycoerythrin (PE), BD Biosciences) for 20 minutes at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous work identified stem/progenitor cells in the deciduas of human first-trimester fetuses by using fluorescent Hoechst dye 33342 to isolate SP cells. These SP cells have been proven to form clones and have differentiated into decidua mesenchymal cells [17,18]. Recently, we found that the subfraction of CD31 - CD146 - SP cells revealed distinct properties, and differentiated into endothelial cells and can be promoted by vascular endothelial growth factor (VEGF), estrogen and progesterone in vitro .…”

Section: Introductionmentioning

confidence: 99%

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Vasculogenesis of decidua side population cells of first-trimester pregnancy

et al. 2013

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IntroductionSufficient uterine blood supply is essential for the fetus to develop normally in the uterus. Several mechanisms are involved in the process of vessel development in deciduas and villus. We focus on whether first-trimester decidua side population (SP) cells contain cells capable of differentiating into endothelial cells.MethodsEight decidua samples were collected from healthy women, 22- to 30-years old, undergoing elective terminations of early pregnancy (six to eight gestational weeks). The cell suspensions from human deciduas were stained by Hoechst 33342 and sorted by flow cytometry, further cultured under differentiation conditions and analyzed for specific markers. These cells were implanted into ischemic limbs of nude mice to test the capacity of angiogenesis in vivo by DiI tracers and immunohistochemistry.ResultsDecidua CD31-CD146- SP cells of first-trimester human pregnancy can differentiate into endothelial cells, express the corresponding specific markers of endothelial cells, such as CD31 and CD146, and form tube-like structures on Matrigel and part of newly formed vessels in the ischemic limbs of nude mice. Vascular endothelial growth factor was more effective in promoting proliferation of CD31-CD146-SP cells compared with other growth factors, and estrogen and progesterone at a final concentration of 10 μmol/L and 30 μmol/L, respectively, promoted the migration of CD31-CD146-SP cells in a dose-dependent manner.ConclusionsCD31-CD146- SP cells may be involved in the formation of new vessels in the maternal aspect of the placenta in the first trimester.

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Side population cells in the human decidua of early pregnancy exhibit stem/progenitor cell-like characteristics

Cited by 6 publications

References 24 publications

Endometrial stem/progenitor cells: the first 10 years

Endometrial stem/progenitor cells: the first 10 years

Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte-associated immunoglobulin-like receptor 1

Vasculogenesis of decidua side population cells of first-trimester pregnancy

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