Side-specific effects by cadmium exposure: Apical and basolateral treatment in a coculture model of the blood–air barrier

Papritz, Mirko; Pohl, Christine; Wübbeke, Christoph; Moisch, Michaela; Hofmann, Hélène; Hermanns, Maria Iris; Thiermann, Horst; Kirkpatrick, Charles James; Kehe, Kai

doi:10.1016/j.taap.2010.04.002

Cited by 25 publications

(15 citation statements)

References 34 publications

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“…Ion transport characteristics of the H441 cells suggest that it forms polarized monolayers, although the polarization appears to be highly variable with the majority of cultures only reaching TEER values of around 300 Ω·cm 2 [172,173]. The NCI-H441 cell line has been used in co-culture models together with endothelial cells in studies aiming to characterize the air-blood barrier, but remains to be tested in co-cultures with immune cells [174,175]. …”

Section: Epithelial Cell Types In Airway Co-culture Modelsmentioning

confidence: 99%

Polarized Airway Epithelial Models for Immunological Co-Culture Studies

Papazian

Würtzen

Hansen

2016

Int Arch Allergy Immunol

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Epithelial cells line all cavities and surfaces throughout the body and play a substantial role in maintaining tissue homeostasis. Asthma and other atopic diseases are increasing worldwide and allergic disorders are hypothesized to be a consequence of a combination of dysregulation of the epithelial response towards environmental antigens and genetic susceptibility, resulting in inflammation and T cell-derived immune responses. In vivo animal models have long been used to study immune homeostasis of the airways but are limited by species restriction and lack of exposure to a natural environment of both potential allergens and microflora. Limitations of these models prompt a need to develop new human cell-based in vitro models. A variety of co-culture systems for modelling the respiratory epithelium exist and are available to the scientific community. The models have become increasingly sophisticated and specific care needs to be taken with regard to cell types, culture medium and culture models, depending on the aim of the study. Although great strides have been made, there is still a need for further optimization, and optimally also for standardization, in order for in vitro co-culture models to become powerful tools in the discovery of key molecules dictating immunity and/or tolerance, and for understanding the complex interplay that takes place between mucosa, airway epithelium and resident or infiltrating immune cells. This review focuses on current knowledge and the advantages and limitations of the different cell types and culture methods used in co-culture models of the human airways.

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Section: Epithelial Cell Types In Airway Co-culture Modelsmentioning

confidence: 99%

Polarized Airway Epithelial Models for Immunological Co-Culture Studies

Papazian

Würtzen

Hansen

2016

Int Arch Allergy Immunol

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“…[6][7][8] A similar cell culture system has been used in a number of other epithelial barrier models, such as the lung air-blood barrier and the bronchial barrier model. [9][10][11][12][13] In the present study with the Caco-2/ISO-HAS-1 cell lines, a characterization of the in vitro barrier was performed using immunofluorescence staining and transmission electron microscopy (TEM) to determine if the respective cell-phenotype, cell-cell contacts, and the morphology of the cells were maintained. The barrier properties of the model were determined by measuring the transepithelial electrical resistance (TER) and the transport velocity of NaFITC across the barrier.…”

Section: Introductionmentioning

confidence: 99%

The role of the intestinal microvasculature in inflammatory bowel disease: studies with a modified Caco-2 model including endothelial cells resembling the intestinal barrier in vitro

Kasper¹,

Hermanns²,

Cavelius³

et al. 2016

IJN

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“…This is an important parameter for studies addressing drug or particle permeability across the tight junctional barrier. Hermanns et al designed a suitable co-culture model composed of the epithelial cell line NCI-H441 and primary isolated human pulmonary microvascular endothelial cells (HPMECs) or the endothelial cell line ISO-HAS-1 to study adsorption, uptake and trafficking of novel nanosized carriers under different physiological conditions [84]. They could show that epithelial cells react to nanocarriers in a completely different manner in the co-culture compared with the simple monolayer model, in terms of barrier properties and inflammatory responses.…”

Section: Advanced Reconstructed Tissue Modelsmentioning

confidence: 99%