Siderophore-assisted cadmium hyperaccumulation in Bacillus subtilis

Khan, Azmi; Gupta, Adity; Singh, Pratika; Mishra, Arun Kumar; Ranjan, Rajesh Kumar; Srivastava, Amrita

doi:10.1007/s10123-019-00101-4

Cited by 46 publications

(15 citation statements)

References 36 publications

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“…There are several investigations on the application of B. subtilis to manage R. solani in several crops (Szczech and Shoda 2006;Ma et al 2015;Mahmoud 2016b;Selim et al 2017;El-Desouky et al 2018). Various mechanisms have been suggested to elucidate the suppression of plant pathogenic fungi by B. subtilis, especially competition for space (Yu et al 2011;Cao et al 2012;Li et al 2013), competition for nutrients (Moita et al 2005;Li et al 2013;Wang et al 2018b), antibiosis (Landy et al 1948;Stein 2005;Wang et al 2018b), siderophore production (Yu et al 2011Singh et al 2017;Khan et al 2020) and induction of systemic resistance (Ongena et al 2005(Ongena et al , 2007Wang et al 2018b), or applied other biocontrol mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Identification, pathogenicity, fungicide sensitivity and biological control of Rhizoctonia solani associated with damping-off disease of sugar beet in Morocco

Farhaoui¹,

Tahiri²,

Ezrari³

et al. 2022

Preprint

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The fungus Rhizoctonia solani Kühn is an economically important pathogen involved in root rot and seedling damping-off disease of sugar beet (Beta vulgaris L.). In Morocco, this is the first detailed report of R. solani as the causative agent of sugar beet damping-off. The identification of the pathogen was based on combining the morphological characteristics and molecular characterization using the ribosomal DNA internal transcribed spacer (rDNA ITS) region. Antifungal activities of four commercial fungicides; azoxystrobin, difenoconazole, tebuconazole, and trifloxystrobin were assessed in vitro, displaying effective inhibition of the mycelial growth of the pathogen strains using PDA medium. A commercially available Bacillus subtilis Y1336 was evaluated as a potential biological control agent (BCA) against seedling damping-off caused by R. solani. The biological control activities of B. subtilis Y1336 was demonstrated by dual culture bioassays on PDA medium, in vitro seed inoculation with R. solani and B. subtilis, and by applying the BCA as seed coat under greenhouse conditions. This is the first time that the commercially available B. subtilis Y1336 has been proved as an effective BCA against the damping-off disease of sugar beet. This commercial antagonist should be incorporated into the integrated disease management startegy for sugar beet seedling mortality together with effective tested fungicides.

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Section: Discussionmentioning

confidence: 99%

Identification, pathogenicity, fungicide sensitivity and biological control of Rhizoctonia solani associated with damping-off disease of sugar beet in Morocco

Farhaoui¹,

Tahiri²,

Ezrari³

et al. 2022

Preprint

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“…Sequential fractions were collected and catecholate positive fractions were pooled together. Methanol was then evaporated using a rotary evaporator (40°C; 110 mbar pressure; Khan et al, 2020 ). Samples were further purified by size exclusion chromatography using Sephadex LH20 resin.…”

Section: Methodsmentioning

confidence: 99%

“…For catecholate siderophore transport in A. nidulans , expected functional partners were analyzed and confirmed using STRING database server 1 ( Khan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Siderophore Isolated From Escherichia coli Against Aspergillus nidulans via Iron-Mediated Oxidative Stress

et al. 2021

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Microorganisms produce various secondary metabolites for growth and survival. During iron stress, they produce secondary metabolites termed siderophores. In the current investigation, antifungal activity of catecholate siderophore produced by Escherichia coli has been assessed against Aspergillus nidulans. Exogenous application of the bacterial siderophore to fungal cultures resulted in decreased colony size, increased filament length, and changes in hyphal branching pattern. Growth inhibition was accompanied with increased intracellular iron content. Scanning electron microscopy revealed dose-dependent alteration in fungal morphology. Fluorescent staining by propidium iodide revealed cell death in concert with growth inhibition with increasing siderophore concentration. Antioxidative enzyme activity was also compromised with significant increase in catalase activity and decrease in ascorbate peroxidase activity. Siderophore-treated cultures showed increased accumulation of reactive oxygen species as observed by fluorescence microscopy and enhanced membrane damage in terms of malondialdehyde content. Antifungal property might thus be attributed to xenosiderophore-mediated iron uptake leading to cell death. STRING analysis showed interaction of MirB (involved in transport of hydroxamate siderophore) and MirA (involved in transport of catecholate siderophore), confirming the possibility of uptake of iron–xenosiderophore complex through fungal transporters. MirA structure was modeled and validated with 95% residues occurring in the allowed region. In silico analysis revealed MirA–Enterobactin–Fe3+ complex formation. Thus, the present study reveals a promising antifungal agent in the form of catecholate siderophore and supports involvement of MirA fungal receptors in xenosiderophore uptake.

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“…B. subtilis strain CAS15 was isolated from the rhizosphere and identified as producing siderophore, and it also inhibits the growth of phytopathogens [ 43 ]. Similarly, the siderophore-mediated bioaccumulation of cadmium (Cd) has been reported in B. subtilis and demonstrated as a substitute bioremediation strategy [ 44 ]. In recent years, the biological control of phytopathogens has been the subject of research, because it helps in limiting the use of hazardous chemically synthesized pesticides.…”

Section: Non-ribosomal Peptides (Nrps)mentioning

confidence: 99%

Classification and Multifaceted Potential of Secondary Metabolites Produced by Bacillus subtilis Group: A Comprehensive Review

et al. 2023

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Despite their remarkable biosynthetic potential, Bacillus subtilis have been widely overlooked. However, their capability to withstand harsh conditions (extreme temperature, Ultraviolet (UV) and γ-radiation, and dehydration) and the promiscuous metabolites they synthesize have created increased commercial interest in them as a therapeutic agent, a food preservative, and a plant-pathogen control agent. Nevertheless, the commercial-scale availability of these metabolites is constrained due to challenges in their accessibility via synthesis and low fermentation yields. In the context of this rising in interest, we comprehensively visualized the antimicrobial peptides produced by B. subtilis and highlighted their prospective applications in various industries. Moreover, we proposed and classified these metabolites produced by the B. subtilis group based on their biosynthetic pathways and chemical structures. The biosynthetic pathway, bioactivity, and chemical structure are discussed in detail for each class. We believe that this review will spark a renewed interest in the often disregarded B. subtilis and its remarkable biosynthetic capabilities.

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Siderophore-assisted cadmium hyperaccumulation in Bacillus subtilis

Cited by 46 publications

References 36 publications

Identification, pathogenicity, fungicide sensitivity and biological control of Rhizoctonia solani associated with damping-off disease of sugar beet in Morocco

Identification, pathogenicity, fungicide sensitivity and biological control of Rhizoctonia solani associated with damping-off disease of sugar beet in Morocco

Antifungal Activity of Siderophore Isolated From Escherichia coli Against Aspergillus nidulans via Iron-Mediated Oxidative Stress

Classification and Multifaceted Potential of Secondary Metabolites Produced by Bacillus subtilis Group: A Comprehensive Review

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