Siderophore-Based Noninvasive Differentiation of Aspergillus fumigatus Colonization and Invasion in Pulmonary Aspergillosis

Luptáková, Dominika; Patil, Rutuja H.; Dobiáš, Radim; Stevens, David A.; Pluháček, Tomáš; Palyzová, Andrea; Káňová, Marcela; Navrátil, Milan; Vrba, Zbyněk; Hubáček, Pavel; Havlı́ček, Vladimı́r

doi:10.1128/spectrum.04068-22

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…Gtx is an immunosuppressive fungal toxin that is secreted to the extracellular environment in response to fungal stress [ 40 ]. Studies have shown the utility of siderophores and/or gliotoxin in human blood and urine as useful biomarkers of Aspergillus infection, and we have detailed this recently [ 36 , 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Aspergillosis in Horses

Dobiáš

Jahn

Tóthová

et al. 2023

JoF

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Invasive pulmonary aspergillosis (IPA) may be a rare cause of granulomatous pneumonia in horses. The mortality of IPA is almost 100%; direct diagnostic tools in horses are needed. Bronchoalveolar lavage fluid (BALF) and serum samples were collected from 18 horses, including individuals suffering from IPA (n = 1), equine asthma (EA, n = 12), and 5 healthy controls. Serum samples were collected from another 6 healthy controls. Samples of BALF (n = 18) were analyzed for Aspergillus spp. DNA, fungal galactomannan (GM), ferricrocin (Fc), triacetylfusarinin C (TafC), and gliotoxin (Gtx). Analysis of 24 serum samples for (1,3)-β-D-glucan (BDG) and GM was performed. Median serum BDG levels were 131 pg/mL in controls and 1142 pg/mL in IPA. Similar trends were observed in BALF samples for GM (Area under the Curve (AUC) = 0.941) and DNA (AUC = 0.941). The fungal secondary metabolite Gtx was detected in IPA BALF and lung tissue samples (86 ng/mL and 2.17 ng/mg, AUC = 1).

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Section: Discussionmentioning

confidence: 99%

Diagnosis of Aspergillosis in Horses

Dobiáš

Jahn

Tóthová

et al. 2023

JoF

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“…This method exhibited a significantly better sensitivity performance than serum sampling. It surpassed the detection accuracy of GM and BDG serology, thus providing a more effective diagnostic tool for invasive pulmonary aspergillosis [29] .…”

Section: Discussionmentioning

confidence: 99%

A nomogram model in invasive pulmonary aspergillosis diagnosis based on a retrospective study

Qian,

Yang,

Wang

et al. 2023

Preprint

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Backgroud:With the easing of governmental COVID-19 restrictions, an upward trend was found for Aspergillus isolation in hospitalized patients . Objectives:Our research aims to analyze the clinical significance of Aspergillus positivity in respiratory tract samples and construct a diagnostic model in clinical practice. Methods: We retrospectively analyzed Aspergillus-positive patients in the three time periods before and after the relaxation of COVID-19 measures. Patients were admitted in into death or survival group to analyze independent risk factors for mortality. Based on the current standards, we divided patients into Invasive pulmonary aspergillosis( IPA) group and colonization group, in an attempt to identify the risk factors for IPA.The independent risk factors of death and IPA were determined through logistic regression analysis.A nomogram model was constructed to evaluate the predictive value of risk factors in IPA diagnosis.Mann–Whitney U test was used to compare the differences between the laboratory indicators of the IPA and the colonization. Resluts: There were 60% (125/208）patients with COVID-19 infection. We found that the overall in-hospital mortality rate was 33.6% (70/208). IPA (p<0.001), old age (p=0.011), mechanical ventilation requirement (p<0.001),and corticosteroids expose (p=0.002) were independent risk factors for mortality, while antifungal treatment had a protective effect (p<0.037).The proportion of IPA in death and survival group were 80% and 48%(p<0.001), respectively.The nomogram was established based on respiratory tract bronchial disease , recurring fever, the use of at least two types of antibiotics, and number of positive Aspergillus culture has significant diagnostic value for IPA(AUC:0.813,sensitivity0.823,specificity:0.714) .Moreover, there were statistical differences between the IPA and colonization group in serum GM level, BDG level, neutrophil absolute value ,blood glucose level , and blood urea nitrogen level(BUN). Conclusion:Due to the rapid progression and high mortality rate of IPA, Aspergillus positivity in respiratory tract samples should be cause for concern.In our study,we created a nomogram model in invasive pulmonary aspergillosis diagnosis. When patients with underlying respiratory diseases , recurring fever and use different types of antibiotics, it is best to monitor of laboratory indicators, such as cultures,GM and BDG tests .If there is a high clinical suspicion, we recommend starting empirical treatment with triazole drugs.

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“…P. aeruginosa PAO1 (ATTC 15692) was purchased from the ATTC collection (Manasses, VA, USA). A. fumigatus strain EI278 was isolated from a clinical sample [ 40 ]. The media used for the growth of monocultures were Luria–Bertani broth (LB, g/L: 10 tryptone, 5 yeast extract, 10 NaCl, pH 7.0) and a minimal iron-limited medium (M9, g/L: 0.5 NaCl, 3.0 KH 2 PO 4 , 14.62 Na 2 HPO 4 × 12 H 2 O, and 1.0 NH 4 Cl, pH 7.0) supplemented with trace elements (M9TE, mg/L: 200 MgSO 4 × 7 H 2 O, 50 CaCl 2 × 2 H 2 O, 10 ZnSO 4 × 7 H 2 O, 17 MnSO 4 × H 2 O, 4.8 CoCl 2 × 6 H 2 O, 3 CuSO 4 × 5 H 2 O, 4.5 Na 2 MoO 4 ) and with glucose as a source of carbon and energy (M9TEGlu, 10 g/L).…”

Section: Methodsmentioning

confidence: 99%

“…aeruginosa PAO1 (ATTC 15692) was purchased from the ATTC collection (Manasses, VA, USA). A. fumigatus strain EI278 was isolated from a clinical sample [40]. The media used for the growth of monocultures were Luria-Bertani broth (LB, g/L: 10 tryptone, 5 yeast extract, 10 NaCl, pH 7.0) and a minimal iron-limited medium (M9, g/L: 0. )…”

Section: Microorganisms and Growth Mediamentioning

confidence: 99%

The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in Pseudomonas aeruginosa

Juříková,

Mácha,

Lupjanová

et al. 2023

Microorganisms

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Pseudomonas aeruginosa is recognized as a significant cause of morbidity and mortality among nosocomial pathogens. In respiratory infections, P. aeruginosa acts not only as a single player but also collaborates with the opportunistic fungal pathogen Aspergillus fumigatus. This study introduced a QS molecule portfolio as a potential new biomarker that affects the secretion of virulence factors and biofilm formation. The quantitative levels of QS molecules, including 3-o-C12-HSL, 3-o-C8-HSL, C4-HSL, C6-HSL, HHQ, PQS, and PYO, measured using mass spectrometry in a monoculture, indicated metabolic changes during the transition from planktonic to sessile cells. In the co-cultures with A. fumigatus, the profile of abundant QS molecules was reduced to 3-o-C12-HSL, C4-HSL, PQS, and PYO. A decrease in C4-HSL by 50% to 170.6 ± 11.8 ng/mL and an increase 3-o-C12-HSL by 30% up to 784.4 ± 0.6 ng/mL were detected at the stage of the coverage of the hyphae with bacteria. Using scanning electron microscopy, we showed the morphological stages of the P. aeruginosa biofilm, such as cell aggregates, maturated biofilm, and cell dispersion. qPCR quantification of the genome equivalents of both microorganisms suggested that they exhibited an interplay strategy rather than antagonism. This is the first study demonstrating the quantitative growth-dependent appearance of QS molecule secretion in a monoculture of P. aeruginosa and a co-culture with A. fumigatus.

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Siderophore-Based Noninvasive Differentiation of Aspergillus fumigatus Colonization and Invasion in Pulmonary Aspergillosis

Cited by 8 publications

References 45 publications

Diagnosis of Aspergillosis in Horses

Diagnosis of Aspergillosis in Horses

A nomogram model in invasive pulmonary aspergillosis diagnosis based on a retrospective study

The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in Pseudomonas aeruginosa

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