Siderophore-Mediated Iron Uptake Promotes Yeast–Bacterial Symbiosis

Abstract

In the present study, siderophore produced by the marine yeast Aureobasidium pullulans was characterized as hydroxamate by chemical and bioassays. The hydroxamate assignment was supported by the appearance of peaks at 1,647.21-1,625.99 cm(-1) and at 1,435.04 cm(-1) in the infrared spectrum. The purified siderophore exhibited specific growth-promoting activity under iron-limited conditions for siderophore auxotrophic probiotic bacteria. Cross-utilization of siderophore indicates a symbiotic relationship between the yeast A. pullulans and the selected probiotic bacterial strains. Statistical optimization of medium components for improved siderophore production in A. pullulans was depicted by response surface methodology. The shift in UV-Vis spectroscopy indicates the photoreactive property and subsequent oxidative cleavage of purified siderophore on exposure to sunlight.

Marine yeast isolation and industrial application

“…In similar research, Murugappan et al . (2012 ) obtained 0.7 mg mL −1 crude siderophore after 134 h of fermentation using a marine yeast strain ( A. pullulans ).…”

“…Siderophores have wide medical, agricultural and environmental applications ( Renshaw et al ., 2002 ). For example, the ability of using siderophores could give the microorganism a competitive advantage over other microorganisms as the siderophore transport system will enable the microorganism to compete effectively for the available irons ( Renshaw et al ., 2002 ; Murugappan et al ., 2012 ). Siderophores was also used to control the growth of some pathogenic bacteria isolated from infected marine fish ( Renshaw et al ., 2002 ).…”

“…In similar research, Murugappan et al . () obtained 0.7 mg mL −1 crude siderophore after 134 h of fermentation using a marine yeast strain ( A. pullulans ). This strain was isolated from seaweed ( Ulva fasciata ) samples that were collected from the coastal region of the Gulf of Mannar, India.…”

The Multiple and Versatile Roles of Aureobasidium pullulans in the Vitivinicultural Sector

“…Siderophores are very interesting molecules for medical, agricultural and environmental applications, and have been of interest in biotechnology. Although only one strain of A. pullulans (HN6.2) has been reported in the literature to produce siderophores, studies of A. pullulans isolates report the effect and role of siderophores in biocontrol processes of plant and human pathogens [1,44,65,[83][84][85][86][87][88].…”

Investigation of Siderophore-Promoted and Reductive Dissolution of Dust in Marine Microenvironments Such as Trichodesmium Colonies

“…The pathways for this bio-enhanced dissolution are still under investigation, but there is evidence suggesting that Trichodesmium colonies employ two bio-dissolution mechanisms: siderophore promoted dissolution and reductive dissolution. Siderophores are low molecular weight organic ligands with a high affinity for Fe, secreted mostly by heterotrophic bacteria (Trick, 1989;Granger and Price, 1999;Rosenberg and Maurice, 2003). Siderophores enhance Fe dissolution rates by forming a surface complex with Fe atoms exposed at the mineral surface, facilitating the breaking of the bonds with the mineral lattice and carrying the Fe into solution as Fe(III)-siderophore complexes (Kraemer et al, 2005).…”