Siderophore Production by Rhizobia Isolated from  Cluster Bean [Cyamopsis tetragonoloba (L.) Taub.] Growing  in Semi-Arid Regions of Haryana, India

Mung bean (Vigna radiata L.) are legume plants that can establish symbiosis with Rhizobacteria to fix N2 from air. Research on isolation, physiological characters and effectiveness of bacteria isolates of root nodules from various plants on the growth of Vigna radiata L has been carried out.The objective of study was to get effective Rhizobacteria isolates which can be potentially used as biological fertilizer agents. Characterization of 16 isolates were carried out and all were included in the fast growing group. For physiological characters of 16 isolates showed that11 isolates were able to produce IAA, 7 isolates were able to solubilize phosphate, 13 isolates were able to produce siderophore, and 16 isolates had protease enzyme and catalase activities. Ten isolates that have growth support activities: AD (1), AD (2), AD (3), AD (4), AD (7), AD (10), LK (2), EKP (1), EKP (2 ), and KcKP (1) were tested for its effectiveness on Vigna radiata L. As a control plant without inoculation on soil media (K1) and plants without being inoculated in plants in soil and compost media (K2).The design used was Completely Randomized Design. The plants were harvested at the age of 55 days. The parameters which were observed were: height of plant, number of leaves, dry weight of canopy, roots, root nodules, total of plants, wet weight of pods, number of pods and chlorophyll content. Inoculation of isolate AD (7) had increased the growth of Vigna radiata L compared to control.

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The Screening of Plant Growth-promoting Rhizobacteria based on the Ability to Promote the Growth and Nodulation of Soybean (Glycine max L.) Seedlings

Purwaningsih¹,

Agustiyani²,

Dewi³

et al. 2023

J. Pure Appl. Microbiol.

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Plant growth-promoting rhizobacteria (PGPR), which were isolated from the plant rhizosphere, decrease the addition of chemical fertilizer (N) and promote plant growth. Some PGPR isolates can fixate nitrogen, solubilize phosphate, produce phytohormones, and control soil pathogens. This study has focused on isolating rhizobacteria from root nodules of Edamame, Glycine max L, Dieng Peanut, Solanum sp. and Peanut root. Eleven isolates were assayed to examine their activities, including the ability of nitrogen fixation, produce Indol Acetic Acid hormone, siderophore, ammonia, and catalase activity. Furthermore, 11 isolates were tested to promote soybean growth in pot experiments using sterile sand media and the test of symbiotic capacity. The results showed that six isolates (RhizE2, RhizE3, RhizE4, RhizKdKbm1, Bio2DW, and Bio3DW) could form root nodules, and four isolates (RhizE2, RhizE3, RhizKdKbm1, and Bio2DW) showed a symbiotic capacity of more than 90%. RhizE2 significantly increased the number of nodules and dry weight of the entire plant (24.08 g) in comparison with plants without inoculation and added N fertilizer (11.59 g) and added N fertilizer (19.60 g). RhizE2 can be further developed as a biological fertilizer agent, especially for soybean plants. From all parameters observed, the plants inoculated with RhizE2 showed the best growth result.

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Siderophore Production by Rhizobia Isolated from Cluster Bean [Cyamopsis tetragonoloba (L.) Taub.] Growing in Semi-Arid Regions of Haryana, India

Cited by 4 publications

References 5 publications

Effect of rhizobial isolates and nitrogen fertilizers on nursery performance, nodulation behavior and nitrogenase activity of Dalbergia sissoo Roxb. seedlings

Effect of rhizobial isolates and nitrogen fertilizers on nursery performance, nodulation behavior and nitrogenase activity of Dalbergia sissoo Roxb. seedlings

Isolation, physiological characters and effectivity of bacterial isolates of root nodules from various plants on the growth of Vigna radiata L

The Screening of Plant Growth-promoting Rhizobacteria based on the Ability to Promote the Growth and Nodulation of Soybean (Glycine max L.) Seedlings

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