SIFAT-SIFAT BIOKIMIAWI EKSTRAK KASAR LIPASE EKSTRASELULER DARI BAKTERI Azospirillum sp. JG3

Abstract

Lipases are valuable biocatalysts because they act under extremely mild conditions, are stable in organic solvents, show broad substrate specificity and exhibit high stereoselectivity. Lipases play important role in various industries such as detergent, cosmetics, flavor, pharmacy and synthesis of organic compounds. The increasing of lipases requirements in industries is goading research to get new lipases resources commited. One of potential lipase resource is Azospirillum sp.JG3 bacteria from Microbiology Laboratory of Biology Faculty University of Jenderal Soedirman. The specific targets of this research are to get crude extract of lipase and investigate its biochemical characteristics. The method used were rejuvenation of Azospirillum sp.JG3 bacteria, inoculum production, determination of optimum production time and bacterium growth phase, extraction and production of lipase to get crude extract, and characterization the biochemical properties of lipase crude extract. The research resulted that crude extract of lipase from Azospirillum sp.JG3 had optimum temperature at 40 °C and optimum pH at pH 7. The lipase was a metalloenzyme with Ca 2+ as its cofactor. The lipase was stable in three organic solvents tested, (chloroform, n-hexane and ether).

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

“…In the previous study, the optimum production time of lipase was achieved at 12 hours incubation, which is the end of the logarithmic phase of bacterial growth phase [9]. The addition of olive oil in the medium has induced the production of lipase enzyme in bacteria cells.…”

“…Production of crude extract lipase was accorded to previous work [9]. Glycerol stock of Azospirillum sp.JG3 bacteria were grown in instant Nutrient Agar medium and incubated for 48 hours at room temperature.…”

“…A positive result is indicated by the orange color fluorescence on medium when irradiated with UV light at a wavelength of 366 nm [8]. A quantitative assay using coconut oil emulsion as substrate showed that crude extract of lipase from this bacteria showed optimum activity at a temperature of 40 °C and pH of 7.0 [9]. In this study, we examined to purify partially the extracellular lipase produced by Azospirillum sp.JG3 using ammonium sulfate fractionation and characterized the fraction that has the highest specific activity.…”

NUTRIENT CONTENT IMPROVEMENT OF TAPIOCA WASTE AND RICE BRAN AS INGREDIENTS TROUGH FERMENTATION USING Azospirillum sp. JG3

“…Lemak dipecah oleh lipase setelah mikrob melampaui fase pertumbuhan eksponensial, atau saat memasuki fase pertumbuhan stasioner (Jaeger, Dijkstra, and Reetz 1999 (Lestari, Handayani, and Oedjijono 2009). Aktivitas tersebut menyebabkan terjadinya akumulasi asam lemak pada media onggok dan dedak sehingga pertumbuhan Azospirillum sp.…”

COMPARATIVE EVALUATION OF CONVENTIONAL VERSUS RAPID METHODS FOR AMPLIFIABLE GENOMIC DNA ISOLATION OF CULTURED <i>Azospirillum</i> sp. JG3

“…In particular, strain Azospirillum sp. JG3 has ability to produce lipase and amylase as recently reported [19][20]. To study the molecular genetics of strain Azospirillum sp.…”