Sığırlarda GSBL ve AmpC Sentezleyen Escherichia coli’nin Prevalansı ve Karakterizasyonu

Abstract

Citation of This ArticleAslantaş Ö, Elmacıoğlu S, Yılmaz EŞ: Prevalence and characterization of ESBL-and AmpC-producing Escherichia coli from cattle. Kafkas Univ Vet Fak Derg, 23, 63-67, 2017. DOI: 10.9775/kvfd.2016 AbstractIn this study, it was aimed to determine the prevalence of extended spectrum β-lactamase (ESBL) and/or AmpC type β-lactamase (AmpC) producing Escherichia coli from cattle in Hatay. For this purpose, 312 rectal swabs samples were collected from apparently healthy cattle. ESBL production was phenotypically investigated by disc combination method and double disc synergism test and β-lactamase genes (blaCTX-M, blaCMY-2, blaSHV, blaOXA, and blaTEM) and plasmid mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS and aac(6')-Ib) were screened by polymerase chain reaction (PCR) and subsequent sequence analysis. Antimicrobial susceptibility of the isolates were determined using disc diffusion method and their phylogenetic groups were also searched by PCR. Twenty six (8.3%) isolates were found to be ESBL producer by phenotypic tests. The following ESBL/AmpC genes were detected: blaCTX-M-15 (n= 12), blaCTX-M-1 (n=11), blaCTX-M-3 (n=2), and blaCMY-2 (n=1). PMQR genes were detected in 11 (42.3%) ESBL producing E. coli isolates and these isolates were only positive for aac(6')-Ib-cr and qnrS1 genes. Twenty two (84.6%) of the isolates exhibited multidrug resistance (MDR) phenotype. ESBL/AmpC producing E. coli isolates were observed to be belonged to B1 (50%), A (34.6%) and D (15.4%) phylogroups. This study was the first to describe the presence of CTX-M-15, CTX-M-3, CTX-M-1 and CMY-2 producing E. coli in cattle in Turkey and the co-existence of aac(6')-Ib-cr and qnrS1 genes in some isolates. Keywords: Escherichia coli, Cattle, ESBL, AmpC Sığırlarda GSBL ve AmpC Sentezleyen Escherichia coli'nin Prevalansı ve Karakterizasyonu ÖzetBu çalışmada, Hatay ilinde sığırlarda genişlemiş spektrumlu β-laktamaz (GSBL) ve/veya plazmid aracılı AmpC tip β-laktamaz (AmpC) sentezleyen E. coli'nin prevalansının belirlenmesi hedeflendi. Bu amaçla, sağlıklı görünüşlü 312 sığırdan rektal sıvab örneği toplandı. Fenotipik GSBL sentezi disk kombinasyon metodu ve çift disk sinerji testi ile β-laktamaz (blaCTX-M, blaCMY-2, blaSHV, blaOXA ve blaTEM) ve plazmid aracılı kinolon direnç (PAKD) genleri (qnrA, qnrB, qnrS ve aac(6')-Ib) polimeraz zincir reaksiyonu (PZR) ve daha sonra sekans analizi ile araştırıldı. Ayrıca, GSBL ve/veya AmpC sentezleyen E. coli izolatlarının antimikrobiyal duyarlılıkları disk difüzyon metodu ile filogenetik gruplarının belirlenmesi ise PZR ile incelendi. Yirmialtı izolat (%8.3) GSBL üretimi yönünden fenotipik testlerle pozitif bulundu. Bu izolatlarda blaCTX-M-15 (n= 12), blaCTX-M-1 (n= 11), blaCTX-M-3 (n= 2) ve blaCMY-2 (n=1) genleri belirlendi. PAKD genleri 11 (%42.3) ESBL sentezleyen E. coli izolatında saptandı ve bu izolatlar sadece aac(6')-Ib-cr ve qnrS1 genleri yönünden pozitif bulundu. İzolatlarının 22'si (%84.6) çoğul direnç fenotipi gösterdi. GSBL/AmpC sentezleyen E. coli izolatlarının B1 (%5...

Sığır ve Koyunlarda Plasmidik AmpC Beta Laktamaz Üreten Escherichia coli Araştırılması ve İzolatların Antibiyogram Profilleri, Filogenetik ve Virulans Karakterizasyonları

“…The real prevalence of pAmpC beta-lactamase producing E. coli is still unknown in healthy cattle and sheep in Turkey. There is only one local study conducted on this topic in healthy cattle in Turkey and it was found only one E. coli isolate carrying ampC (cmy) gene out of 312 cattle rectal swab samples [17] . On the other hand, there is no report for pAmpC beta-lactamase producing E. coli in healthy sheep in Turkey.…”

“…Approximate sample size of the study was determined to be 138 using a 10% expected field prevalence [16,17] at the 95% confidence level and the desired absolute precision of 5% [20] , but more animals were included to the present study for higher precise results. Twenty dairy cattle and 12 sheep farms with no close contact to cattle farms were selected randomly from different locations of Burdur.…”

“…The studies conducted in several countries in the world report the existence and prevalence of AmpC beta-lactamase producing Gram-negative bacteria isolated from livestock [2,[12][13][14][15] . In Turkey, Pehlivanoglu [16] reported the presence of pAmpC producing E. coli in laying hens, Aslantaş et al [17] reported in cattle, Gumus et al [18] reported in dogs and cats and Aslantas and Yilmaz [19] reported in dogs, but more studies are needed to reveal the true prevelance of AmpC producing E. coli in Turkey. Hence, the present study was carried out to investigate the existence of pAmpC betalactamase producing E. coli isolates in healthy cattle and sheep in Burdur, Turkey and to characterize the isolates for antibiotic susceptibility pattern, phylogeny and virulence.…”

Çin’in Sincan Bölgesindeki Buzağılardan İzole Edilen Escherichia coli’nin Filogenetik Gruplandırması ve Antimikrobiyal Direnç Profili

“…Escherichia coli is the most common type of abundant bacteria in human and animal intestines. Some serotypes are pathogenic and can cause diarrhea, meningitis, urinary tract infections, sepsis, or pneumonia in humans and animals [7,8] . Due to the extensive use of antibiotics in veterinary clinics, it is easy for E. coli to evolve resistance to drugs and become a reservoir for antibiotic resistance and resistance genes [9,10] .…”