The chiral effective Lagrangian for pseudoscalar nonet is constructed in consideration of isospin breaking. And the difference of quark condensates is taken accounted.The SU(3) singlet 0 η is not taken as Goldstone-boson. The η mixing with and without isospin symmetry is considered. The quark mass ratios are obtained through solving the mass equations of mesons. We estimate the change of quark mass ratios according to the change of and to see how the electromagnetic corrections affect our results. It turn out that massless up quark is possible. The upper limit for is around 0.39.The values for , quark condensate ratios and other constants are limited in narrow ranges. 0 η M PACS numbers: 12.39.Fe, 14.65.Bt, 14.40.AqKeywords: quark mass ratios, quark condensate, η mixing, chiral perturbation theory ). Previous work in this field ignored the latter and the mass expressions of turned out to be the same [2]. It was explained that the difference between the pionic masses was exclusively generated by electromagnetic interaction , which means that the isospin breaking has no effect on mesonic mass in QCD sector. It is unreasonable.The electromagnetic (EM) corrections for pions and kaons were discussed in Ref.[4], where the correction for pions was comparable to the experimental mass difference. But the result has an uncertainty of 30%. ± π π and 0 * The project is supported in part by Key Research Plan of theoretical Physics and Cross Science of China under Grant No.90503011 , , , . MeV M 136 0 = π MeV M 548 = η MeV M 958 = ′ η