Signal amplification strategies in electrochemiluminescence biosensors

Self-Assembly-Induced Enhancement of Cathodic Electrochemiluminescence of Copper Nanoclusters for a Split-Type Matrix Metalloproteinase 14 Sensing Platform

“…Electrochemiluminescence (ECL) is a phenomenon in which photons are generated through electrochemical reactions . It relies on the redox processes of electrochemical reactions, where electrons or holes are transferred from the electrode to the luminophore in the reaction system at a specific potential, inducing the excited state luminophore to return to its ground state and release photons . As a significant optoelectronic conversion technology, ECL has found extensive applications in various fields such as clinical medicine, , environmental monitoring, , and food analysis , due to its exceptional sensitivity, minimal interference from background signals, and rapid response time .…”

Cu Single-Atom Nanozyme-Mediated Electrochemiluminescence Biosensor for Highly Sensitive Detection of MicroRNA-622

“…However, there are many challenges in its detection. In an attempt to optimize the reliability, reproducibility, and sensitivity of its detection, we aimed to construct a new type of electrochemiluminescence (ECL) biosensor. − ECL is a redox-triggered light emission process . The specific luminescent mechanism and electrochemical reaction process have led to the rapid development of ECL detection technology with the remarkable advantages of outstanding sensitivity and near-zero background. − Additionally, the excellent luminescence quantum yield and stability of luminol have made it a crucial luminophore in the development of ECL systems for biosensing and bioimaging. , In the conventional luminol ECL system, the introduction of hydrogen peroxide (H 2 O 2 ) as the coreactant facilitates the generation of reactive oxygen species (ROSs), such as the hydroxyl radical (OH • ), superoxide radical (O 2 •– ), and singlet oxygen ( 1 O 2 ), which accelerate the oxidation of luminol and amplify the ECL singal. , For this reason, there is a pressing demand for the introduction of efficient catalysts to facilitate the generation of interfacial ROS and construct high-performance ECL biosensors.…”