2018
DOI: 10.1111/jmi.12753
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Signal, noise and resolution in linear and nonlinear structured‐illumination microscopy

Abstract: Structured-illumination microscopy allows widefield fluorescence imaging with resolution beyond the classical diffraction limit. Its linear form extends resolution by a factor of two, and its nonlinear form by an in-principle infinite factor, the effective resolution in practice being determined by noise. In this paper, we analyse the noise properties and achievable resolution of linear and nonlinear 1D and 2D patterned SIM from a frequency-space perspective. We develop an analytical theory for a general case … Show more

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Cited by 49 publications
(54 citation statements)
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“…Hereby the question arises how the performance and resolution of this algorithm scales with a given signal to noise ratio. Since this question has been answered elsewhere, we refer the reader to [30,31] or other more detailed studies [39].…”
Section: Simulationmentioning
confidence: 99%
“…Hereby the question arises how the performance and resolution of this algorithm scales with a given signal to noise ratio. Since this question has been answered elsewhere, we refer the reader to [30,31] or other more detailed studies [39].…”
Section: Simulationmentioning
confidence: 99%
“…The quadratic lattice, which contains two more peaks in the reciprocal domain compared to the sinusoidal fringe, is produced with two pairs of coherent orthogonal beams. The hexagonal lattice is generated in a similar manner, with three pairs of light beams with a high degree of coherence [19]. Due to increased sparsity, the quadratic and hexagonal lattices bear higher modulation depth than sinusoidal fringes in a thick sample, which can yield better reconstruction results.…”
Section: Methodsmentioning
confidence: 99%
“…By combining the normalized cross-correlation method 4 with a spectrum notch, HiFi-SIM could automatically estimate the pattern parameters from most raw data, including low SNR data, TIRF-SIM data, and even data with obvious periodic structures (Supplementary Note 2). Moreover, HiFi-SIM is not sensitive to used OTF and reconstruction parameters 6,18 and HiFi-SIM (Fig. 2).…”
mentioning
confidence: 98%