Signal regulation of proline metabolism in callus of the halophyte Nitraria tangutorum Bobr. grown under salinity stress

Yang, Yingli; Yang, Fan; Li, Xiaoning; Shi, Ruxia; Jin, Lu

doi:10.1007/s11240-012-0209-7

Cited by 37 publications

(15 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since we observed that intracellular calcium ions only increased in root cells after 24 h of exposure to MeJA, the following experiments were done only after 24 h of exposure to MeJA. We used chemicals that either reduce the external concentration of calcium (EGTA), block several calcium channels located in cell membranes (LaCl3) or block IP3dependent Ca channels (heparin) (Yamamoto et al 1990;White 2000;Poutrain et al 2009;Klaas et al 2011;Amelot et al 2012;Yang et al 2013).…”

Section: Calcium Channel Blockers and Calcium Chelator Effect On Mejamentioning

confidence: 99%

“…; Yang et al . ). EGTA binds calcium ions in the apoplastic space to prevent their entry into the cells (Schwartz et al .…”

Section: Introductionmentioning

confidence: 97%

“…Different pharmacological agents have been used to understand the role of changes in [Ca 2+ ]cyt in this process. These include lanthanum chloride (LaCl3) and heparin that can inactivate or block different calcium channels (Poutrain et al 2009;Sun et al 2009;Klaas et al 2011;Amelot et al 2012) or chemicals that can remove the calcium from the medium like ethylene glycol tetraacetic acid (EGTA) (Li et al 2012;Yang et al 2013). EGTA binds calcium ions in the apoplastic space to prevent their entry into the cells (Schwartz et al 1988).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process

Sánchez-Romera

Ruíz-Lozano

et al. 2013

Plant Cell & Environment

104

View full text Add to dashboard Cite

The role of jasmonic acid in the induction of stomatal closure is well known. However, its role in regulating root hydraulic conductivity (L) has not yet been explored. The objectives of the present research were to evaluate how JA regulates L and how calcium and abscisic acid (ABA) could be involved in such regulation. We found that exogenous methyl jasmonate (MeJA) increased L of Phaseolus vulgaris, Solanum lycopersicum and Arabidopsis thaliana roots. Tomato plants defective in JA biosynthesis had lower values of L than wildtype plants, and that L was restored by addition of MeJA. The increase of L by MeJA was accompanied by an increase of the phosphorylation state of the aquaporin PIP2. We observed that MeJA addition increased the concentration of cytosolic calcium and that calcium channel blockers inhibited the rise of L caused by MeJA. Treatment with fluoridone, an inhibitor of ABA biosynthesis, partially inhibited the increase of L caused by MeJA, and tomato plants defective in ABA biosynthesis increased their L after application of MeJA. It is concluded that JA enhances L and that this enhancement is linked to calcium and ABA dependent and independent signalling pathways.

show abstract

Section: Calcium Channel Blockers and Calcium Chelator Effect On Mejamentioning

confidence: 99%

“…; Yang et al . ). EGTA binds calcium ions in the apoplastic space to prevent their entry into the cells (Schwartz et al .…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process

Sánchez-Romera

Ruíz-Lozano

et al. 2013

Plant Cell & Environment

104

View full text Add to dashboard Cite

show abstract

“…However, most research on N. tangutorum has been restricted to its physiological responses to stress environments. For instance, enhanced proline accumulation and elevated levels of calcium in N. tangutorum under NaCl treatment have been reported by Yang et al (2010Yang et al ( , 2013. In contrast, little is known about the molecular mechanisms underlying the perception and adaptability of N. tangutorum to various stressors.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is similar to CIPK2 proteins from other plants, and contains conserved domains and motifs. The promoter of NtCIPK2 harbors many cis-acting elements that might be recognized and bound by transcription factors that are related to hormones and stress responses. NtCIPK2 was ubiquitously and robustly expressed in all tested organs, and was induced by salinity, drought, heat, and cold stress. The overexpression of NtCIPK2 in Escherichia coli caused better growth against high salinity, alkalinity, and osmotic conditions, dehydration, and extreme temperatures (i.e., heat and cold) compared to the control. 4717©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4716-4728 (2014) Cloning and characterization of the NtCIPK2 gene Thus, NtCIPK2 is a candidate gene that might improve the stress tolerance of crops and herbs through genetic manipulation.

show abstract

“…Nitraria tangutorum Bobr. is an important species of desert, sandy, and saline-alkali vegetation in northwestern China and is the winner of long-term natural selection in harsh habitats because it has good adaptability to the extreme environmental conditions of the desert [23]. In recent years, reports on N. tangutorum have mainly focused on the physiological mechanism of drought and salt tolerance [24][25][26][27], which indicated that this species has very important ecological functions.…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of reference genes for quantitative real-time PCR analysis of Nitraria tangutorum

Wang

Duan

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Suitable reference genes can be used to calibrate the error in quantitative real‑time polymerase chain reaction (qRT-PCR) experiments and make the results more credible. However, reference genes suitable for different species and different experimental conditions do not exist. Nitraria tangutorum Bobr. is a typical plant in desert areas and desert plains, which is drought-resistant, saline-alkali resistant, barren-resistant, and has extremely strong adaptability. Due to insufficient understanding of the importance of this germplasm in the past, it is still unclear which genes can be used as reference genes to calibrate qRT-PCR data of N. tangutorum .Results: In this study, we analyzed the expression levels of 10 candidate reference genes (ACT, GAPDH, TUA, TUB, CYP, UBC, His, PP2A, HSP, and EF1-α) in three tissues (root, stem and leaf) and under five abiotic stresses (salt, drought, heat, cold, and ABA) of N. tangutorum seedlings by qRT-PCR. Three analysis software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate expression stability of ten genes. Comprehensive analysis showed that EF1-α and His had the best expression stability, whereas HSP was the least suitable as a reference gene. The expression profile of NtCER7, a gene related to the regulation of the waxy synthesis of N. tangutorum, verified the accuracy of the experimental results.Conclusion: Based on this study, we recommend EF1-α and His as suitable reference genes for N. tangutorum. This study provides the first data on stable reference genes in N. tangutorum, which will be beneficial to study of the gene expression of N. tangutorum and other Nitraria species in the future.

show abstract

Signal regulation of proline metabolism in callus of the halophyte Nitraria tangutorum Bobr. grown under salinity stress

Cited by 37 publications

References 52 publications

Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process

Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

Selection and validation of reference genes for quantitative real-time PCR analysis of Nitraria tangutorum

Contact Info

Product

Resources

About