Signal transduction and cytoskeletal reorganization are required for cell detachment from cell culture surfaces grafted with a temperature‐responsive polymer

Yamato, Masayuki; Okuhara, Minako; Karikusa, Fumiko; Kikuchi, Akihiko; Sakurai, Yasuhisa; Okano, Teruo

doi:10.1002/(sici)1097-4636(199901)44:1<44::aid-jbm5>3.3.co;2-o

Cited by 18 publications

(12 citation statements)

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“…If the force of interaction between the type V collagen fibrils and the surface of the culture dish is weaker than that between the cells and the type V collagen fibrils, then the force exerted by cell motility may result in the cells detaching from the dish. Thus, a retraction force that is exerted on type V collagen fibrils may accelerate the detachment of the fibrils from the surface of the culture dish if the friction between the fibrils and dish surface is weak, as discussed in the case of cell detachment from a dish coated with poly(N-isopropylacrylamide) (Yamato et al 1999(Yamato et al , 2000.…”

Section: Discussionmentioning

confidence: 99%

Reconstituted type V collagen fibrils as cementing materials in the formation of cell clumps in culture

et al. 2004

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Previous studies have reported that type V collagen is an anti-adhesive substrate for cultured cells in that the cells detach from culture dishes coated with type V collagen molecules or polypeptides derived from them. We have noticed that human fetal lung fibroblasts (TIG-1) initially show no reduction in adherence to and spreading on a dish coated with reconstituted type V collagen fibrils but eventually detach from the dish and form cell clumps. To determine the way in which reconstituted type V collagen fibrils are involved in cell clump formation, we have followed the fate of the fluorescence of type V collagen fibrils pre-labeled with fluorescein isothiocyanate. Essentially, all the fluorescence disappeared from the dish surface as the cells detached and was condensed in the cell clumps. The cells that were recovered from clumps and dissociated into separate cells by trypsin treatment proliferated normally after they were seeded on a bare culture dish. This result and those from gel electrophoresis, fluorescence microscopy, and a cell proliferation assay indicate that the cell detachment from the dish is not caused by cell necrosis or apoptosis but by cellular motility together with the unique features of type V collagen fibrils. Not only the adherence of type V collagen fibrils to TIG-1 cells is much stronger than that to the culture dish, but the fibrils are retained on the cellular surface. The strong adherence of type V collagen fibrils to cells plays a role in cementing TIG-1 cells together.

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Section: Discussionmentioning

confidence: 99%

Reconstituted type V collagen fibrils as cementing materials in the formation of cell clumps in culture

et al. 2004

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“…Our postulated mechanism for thermally regulated cell adhesion/detachment on PIPAAm grafted surfaces involves thermally regulated cell detachment based on cellular metabolic activity requiring ATP consumption [2,15,16]. Temperature reduction promotes polymer layer hydration, softening, loss of local mechanical modulus and also cell-surface receptor engagement.…”

Section: Introductionmentioning

confidence: 99%

Preparation of thermoresponsive polymer brush surfaces and their interaction with cells

et al. 2008

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“…72 Yamato et al also found that the detachment of fibronectin from PIPAAm surfaces was unable to be induced by the hydration of PIPAAm chains. 71 Cellular activity can be categorized into two classes: one is chemical (the degradation of matrix components by matrix metalloproteinases and covalently crosslinking by transglutaminase) and the other physical (traction forces).…”

Section: Mechanism Of Cell Detachment From Temperature-responsive Surmentioning

confidence: 99%

Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering

2010

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Temperature-responsive intelligent surfaces, prepared by the modification of an interface mainly with poly(N-isopropylacrylamide) and its derivatives, have been investigated. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated attachment and detachment with cells. The advantage of this system is that cells cultured on such temperature-responsive surfaces can be recovered as single cells and/or confluent cell sheets, while keeping the deposited extracellular matrix intact, simply by lowering the temperature without conventional enzymatic treatment. Here, we focus and compare various methods of producing temperature-responsive surfaces for controlling cell attachment/detachment. Spontaneous cell attachment and detachment using several types of temperature-responsive surfaces are mentioned and various effects, such as film thickness and polymer conformation, are discussed. In addition, the development of the next generation of temperature-responsive surfaces using modifications of the polymer coating to allow for rapid cell recovery is summarized.

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Signal transduction and cytoskeletal reorganization are required for cell detachment from cell culture surfaces grafted with a temperature‐responsive polymer

Cited by 18 publications

References 0 publications

Reconstituted type V collagen fibrils as cementing materials in the formation of cell clumps in culture

Reconstituted type V collagen fibrils as cementing materials in the formation of cell clumps in culture

Preparation of thermoresponsive polymer brush surfaces and their interaction with cells

Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering

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