Signal transduction pathways involved in mechanotransduction in bone cells

Liedert, Astrid; Kaspar, Daniela; Blakytny, Robert; Claes, Lutz; Ignatius, Anita

doi:10.1016/j.bbrc.2006.07.214

Cited by 223 publications

(187 citation statements)

References 35 publications

Supporting

Mentioning

173

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have demonstrated that focal adhesion kinase, Ras, Raf, and MEK are upstream signaling molecules of ERK phosphorylation by fluid shear stress (55). However, it has been reported that upstream activation cascades of ERK are varied depending on both cell type and the type of mechanical stress (56 -59).…”

Section: Discussionmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

Kusuyama

Bandow

Shamoto

et al. 2014

Journal of Biological Chemistry

125

107

View full text Add to dashboard Cite

Background: Low intensity pulsed ultrasound (LIPUS) is a mechanical stimulus clinically used to promote bone fracture healing. Results: LIPUS suppresses adipogenesis and promotes osteogenesis of mesenchyme stem/progenitor cell lines by inhibiting PPAR␥2 through ROCK-Cot/Tpl2-MEK-ERK pathway. Conclusion: LIPUS influences multilineage differentiation of mesenchymal stem and progenitor cells. Significance: LIPUS may be a new clinical approach to chronic bone metabolic disorders, including osteoporosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

Kusuyama

Bandow

Shamoto

et al. 2014

Journal of Biological Chemistry

125

107

View full text Add to dashboard Cite

show abstract

“…21 In osteocytes the mechanically induced synthesis and release of PGE2, are transmitted via the cytoskeleton which is physically linked to ions channels, as well as protein kinase C (PKC) and phospholipase A2. PGE2 mediates the gap junction intercellular communication in response to mechanical strain by increasing the number of functional gap junctions and the amount of connexin43 protein (major component of gap-junction).…”

Section: The Effect Of Mechanical Strain On Soft (Cardiovascular) Andmentioning

confidence: 99%

“…Physiological levels of mechanical stress decrease RANKL expression and increase eNOS expression, which is mediated through ERK1/2. 21 Although the strain also activates c-jun kinase (JNK), JNK inhibitor does not prevent strain effect on either RANKL or eNOS. Reduced display of RANKL by cells present in bone downregulates the local osteoclastogenic potential.…”

Section: The Effect Of Mechanical Strain On Soft (Cardiovascular) Andmentioning

confidence: 99%

The effect of mechanical strain on soft (cardiovascular) and hard (bone) tissues

Boccafoschi

Mosca

Ramella

et al. 2013

Cell Adhesion & Migration

View full text Add to dashboard Cite

“…Recent studies have implicated focal adhesion kinase as a key mediator of mechanically induced bone formation in vivo 38,39 . With respect to signal transduction, several mechanotransduction pathways have been identified through in vitro studies and bone adaptation studies [40][41][42][43][44] . As with the study of mechanosensing mechanisms, the challenge remains to determine the role of these pathways during the various stages of fracture-healing.…”

Section: What Mechanotransduction Mechanisms Are Involved In Fracturementioning

confidence: 99%

Mechanotransduction and Fracture Repair

Morgan¹,

Gleason²,

Hayward³

et al. 2008

Journal of Bone and Joint Surgery

View full text Add to dashboard Cite

Fracture-healing is regulated in part by mechanical factors. Study of the processes by which the mechanical environment of a fracture modulates healing can yield new strategies for the treatment of bone injuries. This article focuses on several key unanswered questions in the study of mechanotransduction and fracture repair. These questions concern identifying the mechanical stimuli that promote bone-healing, defining the mechanisms that are involved in this process, and examining the potential for cross-talk between investigations of mechanotransduction in bone-healing and in healing of other mesenchymally derived tissues. Several approaches to obtain accurate estimates of the mechanical stimuli present within a fracture callus are proposed, and our current understanding of the mechanotransduction processes involved in bone-healing is reviewed. Further study of mechanotransduction mechanisms is needed in order to identify those that are most critical and active during the various phases of fracture repair. A better understanding of the effect of mechanical factors on bone-healing will also benefit the study of healing, regeneration, and engineering of other skeletal tissues. The Mechanical Environment of a Healing FractureFracture-healing is governed by genetic as well as epigenetic factors. The mechanical environment of a healing fracture is one such epigenetic factor that is known to have a profound influence on the rate and success of the repair process. Understanding the effect of the mechanical environment, and in particular the mechanisms by which mechanical cues modulate bone-healing, has applications ranging from clinical management of fractures to bone-tissue engineering and basic science investigations of cell fate.Multiple parameters contribute to the mechanical environment of a fracture callus. These include the stability of fixation, the geometry or type of fracture, and the type of loading. For example, highly stable fixation, such as that provided by a rigidly applied internal fixation plate and by an interfragmentary screw, results in primary cortical healing without the formation of a callus. Less stable external fixation results in a cartilaginous callus, the size of which depends heavily on the stiffness of the fixator frame 1-3 . The geometry or type of fracture affects how the external loads are transferred to the callus tissue. A simple example is the comparison of a transverse fracture line to an oblique fracture line. Even under the same axial compressive Corresponding author: Elise F. Morgan, PhD, Department of Aerospace and Mechanical Engineering, Boston University, 110 Cummington Street, Boston, MA 02215. E-mail address: efmorgan@bu.edu. Disclosure: In support of their research for or preparation of this work, one or more of the authors received, in any one year, outside funding or grants in excess of $10,000 from the National Institutes of Health (grant #AR053353) and the Whitaker Foundation (graduate fellowship) and of less than $10,000 from Boston University (undergraduate ...

show abstract

Signal transduction pathways involved in mechanotransduction in bone cells

Cited by 223 publications

References 35 publications

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

The effect of mechanical strain on soft (cardiovascular) and hard (bone) tissues

Mechanotransduction and Fracture Repair

Contact Info

Product

Resources

About