Signalement van: Spiessens – De namen van de waarheid / Jan van Elzen (1999)

Eenoo, Romain Van

doi:10.21825/wt.v59i3.12946

Cited by 2 publications

(2 citation statements)

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“…To demonstrate excellent biofouling properties of the coating, three sets of analytes with different molar masses were selected for the separations. Beta-blockers are used as drugs for hypertension treatment to lower blood pressure [35] and also as illegal doping to enhance performance [36] and as has been proved earlier, efficient separation of beta-blockers requires capillary coating [37]. For these reasons effective screening systems for beta-blockers are needed.…”

Section: Separation Of Beta-blockers Basic Proteins and Lipoproteinsmentioning

confidence: 99%

Stable neutral double hydrophilic block copolymer capillary coating for capillary electrophoretic separations

et al. 2014

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Quaternized diblock copolymer, poly(N-methyl-2-vinylpyridinium iodide-block-ethylene oxide), was successfully used as a neutral, dynamic coating to suppress the electroosmotic flow. The block copolymer consisted of two polymers that were linked covalently together. The cationic block (poly(N-methyl-2-vinylpyridinium iodide)) was bound efficiently to the negatively charged capillary wall via electrostatic interactions, and the hydrophilic block (ethylene oxide) stabilized the system and created a neutral capillary surface with ultralow electroosmotic flow (+2.0 ± 4.5 × 10(-10) m(2)/Vs). The main advantages of the coating were simple and fast preparation, easy regeneration and automation, and stable electroosmotic flow. To emphasize the potential of this type of coating its stability was measured at a wide pH range demonstrating a high stability in the pH range of 4.0-10.5 and lifetime up to 8 days. The successful studies carried out with beta-blockers, basic proteins, and lipoproteins proved the suitability of the coating for the separation of different sized analytes. Furthermore, the neutral coating developed is useful in a wide range of protein analysis and biological interaction studies under physiological condition.

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Section: Separation Of Beta-blockers Basic Proteins and Lipoproteinsmentioning

confidence: 99%

Stable neutral double hydrophilic block copolymer capillary coating for capillary electrophoretic separations

et al. 2014

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“…In our study, the two novel semi-permanent polyelectrolyte coatings (PECs) were tested with a range of aqueous buffers, the coating thickness was determined by quartz crystal microbalance (QCM), and the distribution constants of a set of alkyl benzoates were calculated. The usability of the coatings was evaluated as a fast screening platform for the separation of cationic ␤-blockers, which are frequently used as illegal doping agents by athletes [21,22]. In addition, the separation of the hydrolysis products of Fig.…”

Section: Introductionmentioning

confidence: 99%

Novel cationic polyelectrolyte coatings for capillary electrophoresis

et al. 2015

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The use of bare fused silica capillary in CE can sometimes be inconvenient due to undesirable effects including adsorption of sample or instability of the EOF. This can often be avoided by coating the inner surface of the capillary. In this work, we present and characterize two novel polyelectrolyte coatings (PECs) poly(2-(methacryloyloxy)ethyl trimethylammonium iodide) (PMOTAI) and poly(3-methyl-1-(4-vinylbenzyl)-imidazolium chloride) (PIL-1) for CE. The coated capillaries were studied using a series of aqueous buffers of varying pH, ionic strength, and composition. Our results show that the investigated polyelectrolytes are usable as semi-permanent (physically adsorbed) coatings with at least five runs stability before a short coating regeneration is necessary. Both PECs showed a considerably decreased stability at pH 11.0. The EOF was higher using Good's buffers than with sodium phosphate buffer at the same pH and ionic strength. The thickness of the PEC layers studied by quartz crystal microbalance was 0.83 and 0.52 nm for PMOTAI and PIL-1, respectively. The hydrophobicity of the PEC layers was determined by analysis of a homologous series of alkyl benzoates and expressed as the distribution constants. Our result demonstrates that both PECs had comparable hydrophobicity, which enabled separation of compounds with log Po/w > 2. The ability to separate cationic drugs was shown with β-blockers, compounds often misused in doping. Both coatings were also able to separate hydrolysis products of the ionic liquid 1,5-diazabicyclo[4.3.0]non-5-ene acetate at highly acidic conditions, where bare fused silica capillaries failed to accomplish the separation.

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Signalement van: Spiessens – De namen van de waarheid / Jan van Elzen (1999)

Cited by 2 publications

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Stable neutral double hydrophilic block copolymer capillary coating for capillary electrophoretic separations

Stable neutral double hydrophilic block copolymer capillary coating for capillary electrophoretic separations

Novel cationic polyelectrolyte coatings for capillary electrophoresis

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