Signaling of reactive oxygen species in PTTH-stimulated ecdysteroidogenesis in prothoracic glands of the silkworm, Bombyx mori

Hsieh, Yun-Chih; Lin, Pei‐Ling; Gu, Shi‐Hong

doi:10.1016/j.jinsphys.2014.02.004

Cited by 14 publications

(9 citation statements)

References 44 publications

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“…Our previous study demonstrated that mitochondrion-derived ROS signaling and Ca 2+ signaling are together involved in PTTH-stimulated ecdysteroidogenesis (Hsieh et al, 2013 ). We further found that ROS signaling lies upstream of ERK, 4E-BP, and AMPK phosphorylation (Hsieh et al, 2014 ). In the present study, we investigated the regulatory function of the redox state on JNK phosphorylation.…”

Section: Discussionmentioning

confidence: 57%

“…In our previous study, we found that PTTH-stimulated phosphorylation of ERK is dependent on the redox status (Hsieh et al, 2014 ). To determine whether the JNK signaling pathway is redox-sensitive, PGs were pretreated with NAC, a superoxide scavenger, to block PTTH-stimulated ROS production (Hsieh et al, 2013 ), and then were stimulated with the PTTH.…”

Section: Resultsmentioning

confidence: 88%

“…This network includes a rapid increase in Ca 2+ (Gu et al, 1998 ; Birkenbeil and Dedos, 2002 ; Fellner et al, 2005 ), cAMP generation (Smith et al, 1984 , 1985 ; Gu et al, 1996 ), and activation of protein kinase A (PKA), phospholipase C (PLC), protein kinase C (PKC), p70S6 kinase (S6K), ribosomal protein S6, and tyrosine kinase (Song and Gilbert, 1995 , 1997 ; Smith et al, 2003 ; Rybczynski and Gilbert, 2006 ; Lin and Gu, 2011 ). Our recent studies further indicated that reactive oxygen species (ROS) and phosphoinositide 3-kinase (PI3K)/adenosine 5′-monophosphate-activated protein kinase (AMPK)/target of rapamycin (TOR) signaling are involved in PTTH-stimulated ecdysteroidogenesis in Bombyx mori PGs (Gu et al, 2011 , 2012 , 2013 ; Hsieh et al, 2013 , 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

Hsieh

et al. 2018

Front. Physiol.

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In this study, phosphorylation of c-Jun N-terminal kinase (JNK) by the prothoracicotropic hormone (PTTH) was investigated in prothoracic glands (PGs) of the silkworm, Bombyx mori. Results showed that JNK phosphorylation was stimulated by the PTTH in time- and dose-dependent manners. In vitro activation of JNK phosphorylation in PGs by the PTTH was also confirmed in an in vivo experiment, in which a PTTH injection greatly increased JNK phosphorylation in PGs of day-6 last instar larvae. JNK phosphorylation caused by PTTH stimulation was greatly inhibited by U73122, a potent and specific inhibitor of phospholipase C (PLC) and an increase in JNK phosphorylation was also detected when PGs were treated with agents (either A23187 or thapsigargin) that directly elevated the intracellular Ca2+ concentration, thereby indicating involvement of PLC and Ca2+. Pretreatment with an inhibitor (U0126) of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) and an inhibitor (LY294002) of phosphoinositide 3-kinase (PI3K) failed to significantly inhibit PTTH-stimulated JNK phosphorylation, indicating that ERK and PI3K were not related to JNK. We further investigated the effect of modulation of the redox state on JNK phosphorylation. In the presence of either an antioxidant (N-acetylcysteine, NAC) or diphenylene iodonium (DPI), PTTH-stimulated JNK phosphorylation was blocked. The JNK kinase inhibitor, SP600125, markedly inhibited PTTH-stimulated JNK phosphorylation and ecdysteroid synthesis. The kinase assay of JNK in PGs confirmed its stimulation by PTTH and inhibition by SP600125. Moreover, PTTH treatment did not affect JNK or Jun mRNA expressions. Based on these findings, we concluded that PTTH stimulates JNK phosphorylation in Ca2+- and PLC-dependent manners and that the redox-regulated JNK signaling pathway is involved in PTTH-stimulated ecdysteroid synthesis in B. mori PGs.

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Section: Discussionmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

Hsieh

et al. 2018

Front. Physiol.

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“…The neuropeptide prothoracicotropic hormone (PTTH) increases from specific physiological stimuli to produce and release ecdysteroids from the prothoractic gland. ROS is then produced through the ecdysteroid signaling pathway, which is stimulated by secretion of PTTH 20 . Our results showed that the expression levels of all BmSODs decreased from the late-larval to the pupal development stages, which coincides with the increased concentration of ecdysteroids in the haemolymph 19 .…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of seven types of superoxide dismutases for their ability to respond to oxidative stress in Bombyx mori

Kobayashi

Nojima

Sakamoto

et al. 2019

Sci Rep

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Insects are well adapted to changing environmental conditions. They have unique systems for eliminating reactive oxygen species (ROS). Superoxide dismutase (SOD) is a key enzyme that plays a primary role in removing ROS. Bombyx mori is a lepidopteran insect, whose body size is larger than the model insect Drosophila melanogaster, which enabled us to more easily examine gene expression at the tissue level. We searched B. mori SOD (BmSOD) genes using genome database, and we analyzed their function under different type of oxidative stress. Consequently, we identified four new types of BmSODs in addition to the three types already known. Two of the seven types had a unique domain architecture that has not been discovered previously in the SOD family, and they were expressed in different tissues and developmental stages. Furthermore, these BmSODs responded differently to several kinds of stressors. Our results showed that the seven types of BmSODs are likely to play different roles in B. mori; therefore, B. mori could be used to distinguish the functions of each SOD for resistance to oxidative stress that changes with the environmental conditions.

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“…In M. sexta, p70S6 kinase (S6K) and ribosomal protein S6 are related to PTTH-stimulated ecdysteroidogenesis (Song and Gilbert, 1997). In addition, extracellular signal-regulated kinase (ERK) phosphorylation, phosphatidylinositol 3-kinase (PI3K)/adenosine 5 0 -monophosphate-acti vated protein kinase (AMPK)/target of rapamycin (TOR) signaling, and reactive oxygen species were found to be involved in PTTH's stimulation of ecdysteroidogenesis (Rybczynski et al, 2001;Lin and Gu, 2007;Gu et al, 2010Gu et al, , 2011Gu et al, , 2012Gu et al, , 2013Hsieh et al, 2013Hsieh et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of orphan nuclear receptor HR38 gene expression by PTTH in prothoracic glands of the silkworm, Bombyx mori

Hsieh

Lin

2016

Journal of Insect Physiology

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Signaling of reactive oxygen species in PTTH-stimulated ecdysteroidogenesis in prothoracic glands of the silkworm, Bombyx mori

Cited by 14 publications

References 44 publications

Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

Comparative analysis of seven types of superoxide dismutases for their ability to respond to oxidative stress in Bombyx mori

Stimulation of orphan nuclear receptor HR38 gene expression by PTTH in prothoracic glands of the silkworm, Bombyx mori

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