Signaling pathways of adipose stem cell-derived exosomes promoting muscle regeneration

Zhu, Aoxuan; Liu, Na; Shang, Yujia; Zhen, Yonghuan; An, Yang

doi:10.1097/cm9.0000000000002404

Cited by 8 publications

(4 citation statements)

References 112 publications

(233 reference statements)

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“…In addition, C-PC has the ability to maintain the differentiation potential of cells by activating the p38-mitogen-activated protein kinase (p38-MAPK) signaling pathway. C-PC could affect the maintenance and improvement of myogenic potential in muscle-derived cells through this signaling pathway. − Sericin is a protein produced by silkworms in silk production. Sericin peptides with a low molecular weight of less than 20 kDa are mainly used in cosmetics, and high molecular weight sericin peptides are used as medical biomaterials and composite polymers .…”

Section: Resultsmentioning

confidence: 99%

Cost-Effective Culture Medium for Cell-Based Future Foods

Park,

Lee,

Jung

et al. 2023

ACS Sustainable Chem. Eng.

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Cell-based protein sources are considered promising future food materials due to their nutritional potential and sustainability. However, the process of massive cell proliferation, which involves an expensive culture medium and is time-consuming, slows the development of cell-based future foods. Herein, we report on the development of cost-effective culture media prepared from egg whites, microalgae, and silk extracts. The developed culture media showed cell proliferation efficiency similar to that of the conventional medium group while maintaining the bovine myoblasts in an undifferentiated state. Furthermore, an improved degree of myogenic differentiation was observed in myoblasts proliferating using the developed culture medium. We selected the optimal cell culture medium for food production by deriving the cost-effectiveness of each culture medium for cell growth. This research provides fundamental insights into building sustainable food systems and cellular agriculture.

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Section: Resultsmentioning

confidence: 99%

Cost-Effective Culture Medium for Cell-Based Future Foods

Park,

Lee,

Jung

et al. 2023

ACS Sustainable Chem. Eng.

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“…They are primarily derived from adipocytes but can also be obtained through liposuction or excised fat 7 . Compared to other mesenchymal stem cells (MSCs), ADSCs have easy access, large quantities, and unique immune regulatory functions 8 . They promote skin regeneration by upregulating the secretion levels of epidermal and vascular endothelial growth factors and play roles in repairing damaged bone tissue and cartilage 9 .…”

Section: Introductionmentioning

confidence: 99%

“…7 Compared to other mesenchymal stem cells (MSCs), ADSCs have easy access, large quantities, and unique immune regulatory functions. 8 They promote skin regeneration by upregulating the secretion levels of epidermal and vascular endothelial growth factors and play roles in repairing damaged bone tissue and cartilage. 9 It has been reported recently that ADSCs facilitate tissue repair through their paracrine action, where exosomes are the major paracrine function products.…”

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confidence: 99%

Adipose stem cell‐derived exosomes promote wound healing by regulating the let‐7i‐5p/GAS7 axis

Liu,

Wang

2024

J of Cosmetic Dermatology

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BackgroundInjury to skin tissue is devastating for human health, making it imperative to devise strategies for hastening wound healing. Normal wound healing is a complex process comprising overlapping steps, including hemostasis, inflammatory response, proliferation, and matrix remodeling. This study investigated the effects of adipose stem cell‐derived exosomes (ADSC‐exos) on wound healing and the underlying mechanisms.MethodsIn vitro hydrogen peroxide (H2O2)‐treated human keratinocyte (HaCaT) cell lines and in vivo animal wound models were established for this purpose. The cell migration was assessed using transwell and wound healing assays, while exosome biomarker expressions were studied using western blot. Moreover, adipose stem cells were identified using flow cytometry, alizarin red S and oil red O staining, and transmission electron microscopy.ResultsResults indicated that H2O2 treatment inhibited the cell viability and migration of HaCaT cells while being promoted by ADSC‐exos. Mechanistic investigations revealed that microRNA‐let‐7i‐5p (let‐7i‐5p) in ADSC‐exos was carried into the HaCaT cells, inhibiting the expression of growth arrest‐specific‐7 (GAS7). Rescue experiments further verified these results, which indicated that GAS7 overexpression reversed the effect of let‐7i‐5p on the viability and migration of HaCaT cells, suggesting ADSC‐exos promoted wound healing via the let‐7i‐5p/GAS7 axis.ConclusionAdipose stem cell‐derived‐exos enhanced the viability and migration of HaCaT via carrying let‐7i‐5p and targeting GAS7, ultimately promoting wound healing in rats.

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“…To the Editor : Adipose-derived stem cells (ASCs) are promising multipotent stem cells for regeneration of skeletal muscle (SM) tissues. [ 1 ] However, the low myogenic differentiation ability limits the application. [ 2 ] Therefore, it is necessary to summarize the current differentiation strategy to improve the differentiation efficiency.…”

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confidence: 99%

Deriving skeletal muscle cells from adipose-derived stem cells: Current differentiation strategies

Liang,

Han,

et al. 2024

Chinese Medical Journal

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Signaling pathways of adipose stem cell-derived exosomes promoting muscle regeneration

Abstract: Are insulin sensitivity and b-cell function associated with adverse pregnancy outcomes among women with gestational diabetes?

Cited by 8 publications

References 112 publications

Cost-Effective Culture Medium for Cell-Based Future Foods

Cost-Effective Culture Medium for Cell-Based Future Foods

Adipose stem cell‐derived exosomes promote wound healing by regulating the let‐7i‐5p/GAS7 axis

Deriving skeletal muscle cells from adipose-derived stem cells: Current differentiation strategies

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