Signalling by the FGFR-like tyrosine kinase, Kringelchen, is essential for bud detachment in<i>Hydra vulgaris</i>

Sudhop, Stefanie; Coulier, François; Bieller, Annette; Vogt, Angelika; Hotz, Tobias; Hassel, Monika

doi:10.1242/dev.01267

Cited by 56 publications

(57 citation statements)

References 42 publications

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“…Though recent transcriptome-wide expression analysis has indicated that VEGF and FGF are predominantly expressed in ectodermal epithelial cells (Hemmrich et al, 2012), we consistently detect VEGF expression exclusively in the endodermal cells and FGF expression in both tissue layers. Previous reports showed the expression of FGFR (Kringelchen) during bud detachment (Sudhop et al, 2004). The localization of FGF, in the budding region of hydra thus suggests its possible role in interacting with FGFR.…”

Section: Homologues Of Vegf and Fgf In Hydra 903mentioning

confidence: 81%

“…Molecular studies have revealed the identification of a signal peptide-containing sequence corresponding to VEGF by cDNA screening in Hydra vulgaris (Böttger et al, 2006). Similarly, charac- Hydra, terization of FGFR (kringelchen) from hydra shows that it participates in bud detachment (Sudhop et al, 2004). These findings strongly suggest the possible presence of VEGF and FGF in hydra.…”

Section: Introductionmentioning

confidence: 97%

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Identification and characterization of VEGF and FGF from Hydra

Krishnapati¹,

Ghaskadbi²

2013

Int. J. Dev. Biol.

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Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) play important roles in the formation of the blood vascular system and in axon guidance, nervous system development and function. Here, we report isolation and characterization of VEGF and FGF homologues from Hydra vulgaris Ind-Pune, a Cnidarian which exhibits an organized nervous system and primitive epithelio-muscular cells. VEGF expression was prominent in the endoderm of the peduncle region and tentacles, as evident from in situ hybridization of whole polyps and its transverse sections. High levels of FGF were detected in the ectoderm of the budding region. The expression of VEGF in endodermal and FGF in interstitial cells was confirmed using sf-1 hydra, a temperature-sensitive mutant strain of Hydra magnipapillata. Tissue-specific expression of VEGF

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Section: Homologues Of Vegf and Fgf In Hydra 903mentioning

confidence: 81%

Section: Introductionmentioning

confidence: 97%

Identification and characterization of VEGF and FGF from Hydra

Krishnapati¹,

Ghaskadbi²

2013

Int. J. Dev. Biol.

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“…These studies identified the differentiation pathways of most cell types in homeostatic and regenerative conditions. In addition, the molecular work performed over the past 20 years has started to dissect the genetic pathways at work during budding, regeneration and reaggregation as the canonical Wnt pathway for maintaining and re-establishing apical organizer activity [33][34][35][36][37][38][39] , the non-canonical Wnt pathway for cellular evagination processes 38 , the MAPK-CREB pathway for triggering head regeneration 46 , the BMP/chordin pathway for axis patterning 47,48 , the FGF pathway for bud detachment 49 , the Notch pathway for differentiating some interstitial cell lineages 50 .…”

Section: Plasticity Of Regeneration In Hydramentioning

confidence: 99%

The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration

Galliot

Chera²

2010

Trends in Cell Biology

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The Hydra model system is well suited to decipher the mechanisms underlying adult regeneration, opening the possibility to characterize those that have been robust enough to be maintained across evolutionary time. The detailed analysis of the activation of the Wnt-βcatenin pathway in bisected Hydra actually shows that the route taken to regenerate a structure as complex as the head dramatically varies according to the amputation level. When decapitation induces a direct redevelopment thanks to Wnt3 signaling from the epithelial cells, head regeneration after midgastric section relies first on Wnt3 signaling from the interstitial cells that undergo apoptosis-induced compensatory proliferation and secondarily activate Wnt3 signaling in the epithelial cells. The relative distribution between stem cells and head progenitor cells is strikingly different in these two contexts indicating that the pre-amputation homeostatic conditions define and constrain the route that bridges wound healing to the re-development program of the missing structure.

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“…Two recent studies have examined the FGF signaling pathway in Cnidaria. Sudhop et al (2004) showed that an FGFR-like gene kringelchen is expressed during bud detachment during asexual reproduction of the hydrozoan Hydra vulgaris and a recent expressed sequence tag (EST) survey of the anthozoans Nematostella vectensis and Acropora millepora yielded several members of FGF signaling pathways . Although these findings suggests that the evolution of FGF signal transduction in the Metazoa predates the evolution of the Bilateria, their expression or role in development has not been characterized.…”

Section: Fgf Signaling In Non-bilateriansmentioning

confidence: 99%

“…With the identification of FGF signal transduction components in N. vectensis and other cnidarians (Sudhop et al 2004;Technau et al 2005), all eumetazoans surveyed possess FGF pathways. Thus, the FGF signal transduction system is an ancient one (Itoh and Ornitz 2004;Popovici et al 2005), predating the cnidarian/bilaterian divergence during the pre-Cambrian (∼600 MYA).…”

Section: Evolution Of the Fgf Familymentioning

confidence: 99%

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

2007

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The fibroblast growth factor (FGF) signal transduction pathway serves as one of the key regulators of early metazoan development, displaying conserved roles in the specification of endodermal, mesodermal, and neural fates during vertebrate development. FGF signals also regulate gastrulation, in part, by triggering epithelial to mesenchymal transitions in embryos of both vertebrates and invertebrates. Thus, FGF signals coordinate gastrulation movements across many different phyla. To help understand the breadth of FGF signaling deployment across the animal kingdom, we have examined the presence and expression of genes encoding FGF pathway components in the anthozoan cnidarian Nematostella vectensis. We isolated three FGF ligands (NvFGF8A, NvFGF8B, and NvFGF1A), two FGF receptors (NvFGFRa and NvFGFRb), and two orthologs of vertebrate FGF responsive genes, Sprouty (NvSprouty), an inhibitor of FGF signaling, and Churchill (NvChurchill), a Zn finger transcription factor. We found these FGF ligands, receptors, and response gene expressed asymmetrically along the oral/aboral axis during gastrulation and in a developing chemosensory structure of planula stages known as the apical tuft. These results suggest a conserved role for FGF signaling molecules in coordinating both gastrulation and neural induction that predates the Cambrian explosion and the origins of the Bilateria.

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Signalling by the FGFR-like tyrosine kinase, Kringelchen, is essential for bud detachment inHydra vulgaris

Cited by 56 publications

References 42 publications

Identification and characterization of VEGF and FGF from Hydra

Identification and characterization of VEGF and FGF from Hydra

The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

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