Significado prognóstico das micrometástases nos linfonodos do carcinoma colorretal: detecção imunoistoquímica com anticorpos anticitoqueratina AE1/AE3

Palma, Rogério Tadeu; Waisberg, Jaques; Simões, Angélica Braz; Bromberg, Sansom Henrique; Appolonio, Fernanda

doi:10.1590/s0100-69912002000300003

Cited by 1 publication

(2 citation statements)

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“…However, even with advances in diagnostic and therapeutic methods such as surgery, chemotherapy and radiotherapy, the five-year survival rate remains around 40% 10 . When treatment is performed in the early stages, this survival rate can reach 80%, whereas in cases of advanced disease, only 20% will still be alive after five years 17 .…”

Section: Discussionmentioning

confidence: 99%

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Association between human Papillomavirus and colorectal adenocarcinoma and its influence on tumor staging and degree of cell differentiation

Junior

Oliveira²,

Freire³

et al. 2014

ABCD, arq. bras. cir. dig.

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BackgroundColorectal cancer is one of the most common types of neoplasia among the worldwide adult population. Among neoplasms of the gastrointestinal tract, it is ranked second in relation to prevalence and mortality, but its etiology is only known in around 5% of the cases. It is believed that 15% of malignant diseases are related to viral oncogenesis.AimTo correlate the presence of HPV with the staging and degree of cell differentiation among patients with colorectal adenocarcinoma.MethodsA retrospective case-control study was conducted on 144 patients divided between a test group of 79 cases of colorectal cancer and a control group to analyze 144 patients aged 25 to 85 years (mean, 57.85 years; standard deviation, 15.27 years and median, 58 years). Eighty-six patients (59.7%) were male. For both groups, tissue samples from paraffin blocks were subjected to DNA extraction followed by the polymerase chain reaction using generic and specific primers for HPV 16 and 18. Dot blot hybridization was also performed with the aim of identifying HPV DNA.ResultsThe groups were shown to be homogenous regarding sex, age and site of HPV findings in the samples analyzed. Out of the 41 patients with HPV, 36 (45.6%) were in the cases and five (7.7%) were in the control group (p<0.001). All the HPV cases observed comprised HPV 16, and HPV 18 was not shown in any of the cases studied. There were no significant differences in comparisons of sex, age and site regarding the presence of HPV in either of the groups. It was not observe any significant difference in relation to staging or degree of cell differentiation among the patients with colorectal cancer.ConclusionHuman papillomavirus type 16 is present in individuals with colorectal carcinoma. However, its presence was unrelated to staging or degree of differentiation.

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Section: Discussionmentioning

confidence: 99%

“…Initially in this study, PCR was performed using generic primers for HPV (GP5+/GP6+) 17 , which were able to amplify 140 bp of the HPV L1 gene. After this procedure, the samples were subjected to Dot Blot hybridization in order to identify HPV types 6, 11, 16, 18, 31, 33, 34, 35, 39, 40, 42, 43, 44, 45, 51, 52, 54, 56 and 58.…”

Section: Discussionmentioning

confidence: 99%